Advances in technologies to detect the seed vigor of grasses

doi:10.11686/cyxb2023043

Abstract

Abstract:

Grass seeds are the fundamental materials for creating artificial grassland， improving the ecological environment of degraded grassland， and ensuring the production potential of grassland for grazing animals. Seed vigor directly affects the production and development of the grassland industry. In China and abroad， seed vigor detection technology has always been an important focus of seed science and technology. However， few studies have focused on the application of seed vigor detection technologies to grass seeds. At present， seed vigor detection technology in grasses includes destructive and non-destructive detection. In this review， we describe recent advances in seed vigor detection in grasses. We introduce destructive detection technologies based on seed germination behavior and physiological and biochemical characteristics， and non-destructive detection technologies based on optical characteristics and appearance characteristics. Additionally， we discuss trends in the development and potential applications of non-destructive technologies to assess seed vigor in grasses. The results of such analyses can provide a reference for the accurate determination of grass seed vigor.

Key words: grass, seed vigor, destructive detection technology, optical characteristics, non-destructive detection technology

Hui-fang YAN, Yu-ting NIE, Li-li CONG, Zhao ZHANG, Kai-lun CUI, Yan-zhen LYU, Mao-feng CHAI. Advances in technologies to detect the seed vigor of grasses[J]. Acta Prataculturae Sinica, 2023, 32(11): 199-211.

Figures/Tables 2

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检测依据 Detection basis	检测技术 Detection technology	优点 Advantage	缺点 Disadvantage
基于种子发芽行为的草种子活力有损检测技术Destructive detection technology of seed vigor in grass based on seed germination behavior	人工加速老化法Artificial accelerated aging method	短期、快速、可靠Short-term， fast and reliable	种间差异大，反映耐贮藏性；不可逆损伤Large difference between species， reflecting seed storage tolerance， irreversible destruction
	低温萌发法Low-temperature germination method	操作简单，较全面反映种子活力综合表现Simple operation and reflecting the comprehensive performance of seed vigor	样本消耗，不可逆损伤Sample consumption and irreversible destruction
	胚根突出法Radicle protrusion method	操作简单、重复性好、节约时间Simple operation， good repeatability and time saving	样本消耗，不可逆损伤Sample consumption and irreversible destruction
基于生理生化特性的草种子活力有损检测技术Destructive detection technology of seed vigor in grass based on physiological and biochemical characteristics	TTC染色法TTC staining method	操作简单、快速省时、结果准确性高Simple operation， fast speed and time saving， and high accuracy of results	不可逆损伤，结果受人员操作、取样标准、样品处理方式影响较大Irreversible destruction. Detection results are greatly affected by personnel operation， sampling standard and sample treatment method
	呼吸强度测定法Respiratory intensity measurement	灵敏性高High sensitivity	专一性太强Too much specificity
	ATP含量测定法ATP content assay	灵敏性高High sensitivity	专一性太强Too much specificity
基于光学特性的草种子活力无损检测技术Non-destructive detection technology of seed vigor in grass based on optical characteristics	近红外光谱检测技术Near-infrared spectroscopy detection technology	无损、成本低、无污染、速度快、耗时短、检测方便、重复性好Non-destruction， low cost， non-pollution， fast speed， short-time consumption， convenient detection and good repeatability	检测结果受水分、温度、样本差异等因素干扰Detection results are affected by water， temperature， sample difference and other factors
	多光谱成像技术Multispectral imaging technology	无损、分辨率高、效率高、污染少、耗时短、可重复性好Non-destruction， high resolution， high efficiency， less pollution， short-time consumption and good repeatability	需选取特征波长以去除图像噪声Characteristic wavelength should be selected to remove image noise
	X光光谱检测技术X-ray spectrum detection technology	无损、效率高、准确率高Non-destruction， high efficiency and high accuracy	检测设备成本高，X光具辐射性，推广使用困难High cost of detection equipment and radioactive X-ray make it difficult to popularize and use
	光声光谱检测技术Photoacoustic spectroscopy detection technology	灵敏度高、精确度高High sensitivity and high accuracy	应用较少Less application
	色选技术Color sorting technology	无损、无污染、简洁、快速Non-destruction， non-pollution， simpleness and fast speed	应用较少Less application
	激光散斑技术Laser speckle techniques	无损、无污染Non-destruction and non-pollution	需克服样本差异性Sample differences are needed to be overcome
	叶绿素荧光检测技术Chlorophyll fluorescence sorting method	无损、无污染、效率高 Non-destruction， non-pollution and high efficiency	样本叶绿素过高/过低导致检测结果欠佳Too high/too low chlorophyll in samples result in poor detection results
基于生理生化特性的草种子活力无损检测技术Non-destructive detection technology of seed vigor in grass based on physiological and biochemical characteristics	电导率法Conductivity method	简单、快速Simpleness and fast speed	复现性差，检测结果受环境因素（种子预处理、水质等）影响较大Poor reproducibility. Detection results are greatly affected by environmental factors （seed pretreatment， water quality， etc.）
基于生理生化特性的草种子活力无损检测技术Non-destructive detection technology of seed vigor in grass based on physiological and biochemical characteristics	电子鼻检测技术Electronic nose detection technology	无损、效率高、快速 Non-destruction， high efficiency and fast speed	检测设备成本高High cost of detection equipment
	H₂O₂流速检测技术Detection technology of H₂O₂ flow rate	无损Non-destruction	检测结果受试剂用量、种子实验区域、检测技术影响Detection results are affected by reagent dosage， seed experiment area and detection technology
	可调谐半导体激光吸收光谱技术Tunable diode laser absorption spectroscopy	无损、灵敏度和分辨率高、实时性强，检测极限高、成本相对低Non-destruction， high sensitivity and resolution， high real time， high detection limit and relatively low cost	尚处于实验室阶段，与农业实际检测存在差距It is still in the laboratory stage， and there is a gap with the actual agricultural detection
基于外观特征的草种子活力无损检测技术Non-destructive detection technology of seed vigor in grass based on appearance characteristics	种子物理性状测定法Determination of seed physical properties	无损、直观Non-destruction and perceptual intuition	不确定性，受种子批收获条件、贮藏条件影响The uncertainty is affected by harvest conditions and storage conditions of seed batch
	机器视觉检测技术Machine vision inspection technology	无损、快速Non-destruction and fast speed	对种子检测环境要求高，需不断试验以确定拍摄区域High requirements for seed detection environment. Continuous experiments are needed to determine the shooting area

检测依据 Detection basis	检测技术 Detection technology	优点 Advantage	缺点 Disadvantage
基于种子发芽行为的草种子活力有损检测技术Destructive detection technology of seed vigor in grass based on seed germination behavior	人工加速老化法Artificial accelerated aging method	短期、快速、可靠Short-term， fast and reliable	种间差异大，反映耐贮藏性；不可逆损伤Large difference between species， reflecting seed storage tolerance， irreversible destruction
	低温萌发法Low-temperature germination method	操作简单，较全面反映种子活力综合表现Simple operation and reflecting the comprehensive performance of seed vigor	样本消耗，不可逆损伤Sample consumption and irreversible destruction
	胚根突出法Radicle protrusion method	操作简单、重复性好、节约时间Simple operation， good repeatability and time saving	样本消耗，不可逆损伤Sample consumption and irreversible destruction
基于生理生化特性的草种子活力有损检测技术Destructive detection technology of seed vigor in grass based on physiological and biochemical characteristics	TTC染色法TTC staining method	操作简单、快速省时、结果准确性高Simple operation， fast speed and time saving， and high accuracy of results	不可逆损伤，结果受人员操作、取样标准、样品处理方式影响较大Irreversible destruction. Detection results are greatly affected by personnel operation， sampling standard and sample treatment method
	呼吸强度测定法Respiratory intensity measurement	灵敏性高High sensitivity	专一性太强Too much specificity
	ATP含量测定法ATP content assay	灵敏性高High sensitivity	专一性太强Too much specificity
基于光学特性的草种子活力无损检测技术Non-destructive detection technology of seed vigor in grass based on optical characteristics	近红外光谱检测技术Near-infrared spectroscopy detection technology	无损、成本低、无污染、速度快、耗时短、检测方便、重复性好Non-destruction， low cost， non-pollution， fast speed， short-time consumption， convenient detection and good repeatability	检测结果受水分、温度、样本差异等因素干扰Detection results are affected by water， temperature， sample difference and other factors
	多光谱成像技术Multispectral imaging technology	无损、分辨率高、效率高、污染少、耗时短、可重复性好Non-destruction， high resolution， high efficiency， less pollution， short-time consumption and good repeatability	需选取特征波长以去除图像噪声Characteristic wavelength should be selected to remove image noise
	X光光谱检测技术X-ray spectrum detection technology	无损、效率高、准确率高Non-destruction， high efficiency and high accuracy	检测设备成本高，X光具辐射性，推广使用困难High cost of detection equipment and radioactive X-ray make it difficult to popularize and use
	光声光谱检测技术Photoacoustic spectroscopy detection technology	灵敏度高、精确度高High sensitivity and high accuracy	应用较少Less application
	色选技术Color sorting technology	无损、无污染、简洁、快速Non-destruction， non-pollution， simpleness and fast speed	应用较少Less application
	激光散斑技术Laser speckle techniques	无损、无污染Non-destruction and non-pollution	需克服样本差异性Sample differences are needed to be overcome
	叶绿素荧光检测技术Chlorophyll fluorescence sorting method	无损、无污染、效率高 Non-destruction， non-pollution and high efficiency	样本叶绿素过高/过低导致检测结果欠佳Too high/too low chlorophyll in samples result in poor detection results
基于生理生化特性的草种子活力无损检测技术Non-destructive detection technology of seed vigor in grass based on physiological and biochemical characteristics	电导率法Conductivity method	简单、快速Simpleness and fast speed	复现性差，检测结果受环境因素（种子预处理、水质等）影响较大Poor reproducibility. Detection results are greatly affected by environmental factors （seed pretreatment， water quality， etc.）
基于生理生化特性的草种子活力无损检测技术Non-destructive detection technology of seed vigor in grass based on physiological and biochemical characteristics	电子鼻检测技术Electronic nose detection technology	无损、效率高、快速 Non-destruction， high efficiency and fast speed	检测设备成本高High cost of detection equipment
	H₂O₂流速检测技术Detection technology of H₂O₂ flow rate	无损Non-destruction	检测结果受试剂用量、种子实验区域、检测技术影响Detection results are affected by reagent dosage， seed experiment area and detection technology
	可调谐半导体激光吸收光谱技术Tunable diode laser absorption spectroscopy	无损、灵敏度和分辨率高、实时性强，检测极限高、成本相对低Non-destruction， high sensitivity and resolution， high real time， high detection limit and relatively low cost	尚处于实验室阶段，与农业实际检测存在差距It is still in the laboratory stage， and there is a gap with the actual agricultural detection
基于外观特征的草种子活力无损检测技术Non-destructive detection technology of seed vigor in grass based on appearance characteristics	种子物理性状测定法Determination of seed physical properties	无损、直观Non-destruction and perceptual intuition	不确定性，受种子批收获条件、贮藏条件影响The uncertainty is affected by harvest conditions and storage conditions of seed batch
	机器视觉检测技术Machine vision inspection technology	无损、快速Non-destruction and fast speed	对种子检测环境要求高，需不断试验以确定拍摄区域High requirements for seed detection environment. Continuous experiments are needed to determine the shooting area

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