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    20 November 2023, Volume 32 Issue 11
    Coupling and coordination relationship between grassland ecosystem service value and ecological well-being of pastoral residents, and its spatial heterogeneity
    Hao CHEN, Fang GONG, Du-lan CHEN
    2023, 32(11):  1-16.  DOI: 10.11686/cyxb2023017
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    Based on the catastrophe theory, a depreciation rate model of ecosystem service value was constructed to estimate the net value of grassland ecosystem services. The ecological well-being index was used to evaluate the ecological well-being level of pastoral residents. A coupling coordination degree model was used to measure the coupling coordination degree between ecosystem service value and the ecological well-being of residents, and further analyses clarified the spatial differences. The research area consisted of 17 counties (cities) in Inner Mongolia where animal husbandry is the main activity. Statistical data from 2012 to 2020 were used for analyses. It was found that: 1) The net value of grassland ecosystem services in the study area increased by 22.9% during the 8 years from 2012 to 2020. The ecological environments of typical grassland, desert grassland, and meadow grassland in Inner Mongolia have been improved to varying degrees. 2) The ecological well-being of pastoral residents in the study area showed a pattern of initially decreasing and then increasing during the 8 years. Specifically, the ecological well-being of residents in the typical grassland area of Inner Mongolia showed a trend of initial decrease and then increase, while those of residents in the desert grassland area and meadow grassland area of Inner Mongolia showed a fluctuating upward trend. 3) The coupling coordination degree between the net value of grassland ecosystem services in the study area and the ecological well-being of residents in pastoral areas significantly increased during the 8-year period. The trend of high-quality coordinated development of typical grassland areas in Inner Mongolia has gradually increased; While trends of mild imbalance and good coordination were detected for the desert steppe. The coupling coordination relationship between the two counties (cities) in the meadow steppe area has effectively improved over time.

    Effect of short-term nutrient addition on aboveground biomass, plant diversity, and functional traits of swampy alpine meadow in Tibet
    Juan-juan ZHOU, Yun-fei LIU, Jing-long WANG, Wei WEI
    2023, 32(11):  17-29.  DOI: 10.11686/cyxb2023048
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    Nitrogen (N) and phosphorus (P) are major nutrients required for plant growth and development. The addition of nutrients plays a key role in the improvement and rejuvenation of degraded grassland. In this study, we investigated the effect of short-term nutrient addition on the aboveground biomass, plant diversity, and functional traits of swampy Kobresia littledalei alpine meadows, either under long-term enclosure or mowed conditions. The study site was located in Jiaduo village of Damxung County, and the study began in April 2019. We also aimed to clarify the contributions of plant diversity and community-level functional traits to changes in aboveground biomass. The experiment consisted of short-term nutrient addition with a control (no nutrient addition) and four treatments: low-level N, high-level N, low-level N+P, and high-level N+P. Fertilizer was added in mid-May and early July in 2019 and 2020. It was found that the aboveground hay yield was increased by at least 39.3% and 98.9% by the addition of N and N+P, respectively. The aboveground biomass of Kobresia royleanaBlysmus sinocompressus, grass groups, and forb groups significantly increased after adding N+P, significantly more so than after adding N separately (P<0.05). The importance value of K. littledalei decreased, while the importance values of K. royleanaPoa sp., Potentilla anserine, and Primula tibetica increased. The addition of N+P significantly increased the species richness, Shannon-Wiener index, and Simpson’s index. Species richness and the Shannon-Wiener index showed highly significant positive correlations with aboveground biomass (P<0.01). Nutrient addition increased the plant height and N content at the community level (P<0.05), N+P addition significantly increased the leaf P content at the community level, increased leaf mass per unit area, and decreased leaf dry matter content. Plant height, leaf P content, and leaf N content were positively correlated with the aboveground biomass at the community level (P<0.01). The results of least squares structural equation and variance decomposition analyses showed that N addition had a significant positive effect on community-level weighted traits and aboveground biomass (P<0.05), while P addition had significant positive effects on community-level weighted traits, aboveground biomass, and plant diversity (P<0.05). The addition of N+P jointly determined the increase in aboveground biomass. The total contribution of plant functional traits and plant diversity to explanation of variation in aboveground biomass at the community level was 81.3%. The contribution of plant functional traits to explanation of variation in aboveground biomass at the community level was 78.5%, and that of plant diversity was 45.5%. In conclusion, plant functional traits and plant diversity jointly determined the response of aboveground biomass to N and P addition at the community level in swampy alpine meadow. The addition of N+P fertilizer had positive effects on the yield and biodiversity of alpine meadow grassland.

    Effects of precipitation on the stability of aboveground biomass in desert steppe
    Xu-dong WU, Qi JIANG, Zhan-jun WANG, Bo JI, Xiao-bin REN
    2023, 32(11):  30-39.  DOI: 10.11686/cyxb2022135
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    Based on the field precipitation control experiment from 2019 to 2021, this research studied the effects of different precipitation levels; Namely, simulated increased (+50%) precipitation, natural precipitation (CK) and precipitation reduction (-50%) on the stability of above-ground biomass in desert grasslands. This field study was conducted at Dashuikeng Grassland Research Station, Yanchi County (106°58' E, 37°24' N, average elevation, 1560 m), Ningxia Hui Autonomous Region, China. It was found that: 1) Agropyron mongolicum determined its dominant position in the community through its adaptability to different rainfall regimes. Lespedeza davurica had the best drought resistance performance. Extreme drought led to a significant decline in the dominance status of Artemisia scoparia and Stipa breviflora, which are perennial species. Poaceous plants were more sensitive to rainfall. 2) Precipitation enhancement significantly improved the stability of the aboveground biomass of communities, perennial legumes, some annual plants or perennial weeds, and L. davurica and A. scopariaP<0.001). Extreme drought treatment significantly reduced the stability of the aboveground biomass of perennial grasses, A. mongolicum and S. brevifloraP<0.001), but a compensation effect among different response functional groups maintained community stability. 3) The response mechanism of compensation between species to maintain the stability of the above-ground biomass determined the response of the desert steppe to extreme drought. By reducing the temporal variability of above-ground biomass under drought conditions, these responses, including compensation among functional groups, promote and maintain the stability of the desert grassland ecosystem.

    Factors affecting the soil erosion and scouring resistance of bank hedgerows in purple soil sloping cropland
    Hong LI, Yun CHEN, Xiao-hong LIU, You-bin LIU, Yi-zhi DU
    2023, 32(11):  40-52.  DOI: 10.11686/cyxb2023006
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    The purpose of this study was to investigate the differences in soil resistance to erosion and scouring for two different bank hedgerow species in purple soil sloping cropland. A systematic study was conducted to investigate the resistance of soil to erosion and scouring for two hedgerow species (Dolichos lablab and Medicago sativa), and to elucidate the coupling relationship between soil erosion and scouring, root parameters and soil physicochemical properties. Unplanted hedge ridges were used as an experiment control (CK). It was found that: 1) The root length, root surface area and soil organic matter content of D. lablab were significantly higher than those of M. sativa, and the soil moisture content, total porosity and non-capillary porosity were significantly higher than those of M. sativa and CK (P<0.05). 2) The mean weight diameter and geometrical mean diameter of water stability aggregate and agglomeration degree of microaggregates ranked D. lablab>M. sativa. Conversely, the percentage of aggregate disruption and dispersive coefficient of microaggregates was greater for M. sativa than for D. lablab. 3) The scouring-resistance index and the change in the scouring-resistance index were greater in D. lablab than in M. sativa. Logarithmic functions could be used to describe the relationships between the scouring-resistance index and time. Quadratic functions could be used to describe the relationships between the change in the scouring-resistance index with time. 4) The results of a redundancy analysis showed that root branches, root tips, soil organic matter and non-capillary porosity had the greatest effect on soil erosion-resistance and scouring resistance. The results of a stepwise regression showed that the percentage of aggregate disruption of water-stable aggregates >0.5 mm was the most influential factor in soil scouring-resistance. In conclusion, soil erosion-resistance and scouring-resistance of D. lablab hedgerows were better than those of M. sativa. Our results provide technical information for the effective establishment of bank hedgerows in purple soil sloping cropland.

    Geometric distribution, formation, and topological structure of soil aggregates after introduction of Caragana korshinskii on the desert steppe
    Zhi-hao ZHU, Chen MENG, Xing WANG, Nai-ping SONG, Li WANG, Miao-miao XU, Ling-tong DU
    2023, 32(11):  53-64.  DOI: 10.11686/cyxb2023011
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    The aims of this work were to understand the distribution, formation, and topological structure of soil aggregates after the artificial establishment of Caragana korshinskii shrubland, and to understand changes in soil ecology and function after the introduction and growth of C. korshinskii. Ultimately, the goal is to guide the formulation of strategies to manage C. korshinskii shrubland and soil resources. C. korshinskii shrublands of five different ages (0, 9, 14, 24, and 35 years old) in the desert steppe area of Yanchi County, Ningxia, were selected as the study sites. The geometric distribution and topology of soil aggregates in C. korshinskii shrubland of different ages were studied by CT scanning and image analyses. With increasing time after the introduction of C. korshinskii, the number, length, volume, surface area, and average equivalent diameter of soil aggregates showed increasing trends, while the average sphericity of aggregates decreased. The number of soil aggregates increased with greater soil depth, but the average equivalent diameter and average sphericity of aggregates decreased with greater soil depth. The introduction and continuous restoration of C. korshinskii were conducive to the formation of surface soil aggregates (especially large aggregates), but led to a decrease in the average sphericity of aggregates. The morphology and stability of aggregates formed in the soil after the introduction of C. korshinskii shrubland differed from those of grassland soil aggregates. Further studies should focus on which morphological characteristics are related to aggregate stability, as this is of great significance for understanding the formation of soil aggregates and their ecological functions.

    Effect of the metabolites in rhizosphere soil on microbial communities of crop intercropping system
    Dai-xiang XU, Jian-feng YANG, Hang SU, Jian-rong ZHAI, Cai QI, Long-gang ZHAO, Yan-jun GUO
    2023, 32(11):  65-80.  DOI: 10.11686/cyxb2023005
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    In order to explore the reasons why interspecific interactions affects crop yield, we comprehensively analyzed the crop yield, the soil physicochemical properties, the rhizosphere soil microbial communities and the root exudates in maize (Zea mays)-soybean (Glycine max) and sorghum (Sorghum bicolor)-soybean intercropping systems in different years, combining the Eco-Biolog microplate method and liquid chromatography-mass spectrometry (LC-MS). Crop yield was significantly increased in the intercropping systems, and the increase of yield in 2020 was more significant than it in 2019. Compared with monoculture, intercropping increased the accumulation and absorption of available nutrients. The contents of available nutrients in the crop rhizosphere soil of the intercropping system were significantly increased. Compared with monoculture, microbial biomass carbon and nitrogen contents in maize, sorghum and soybean in intercropping systems were increased significantly; microorganism activities were also higher, and microbial community composition was more diverse in the rhizosphere soil of intercropped crops. Based on the metabolomic analysis, five key differential metabolites that might cause changes in microbial communities in maize rhizosphere soil were tentatively identified. Four of these appear to enrich the microorganism populations in maize rhizosphere soil, and one appears to be inhibitory. Three metabolites in sorghum rhizosphere soil were identified, two of which appear to enrich presence of microorganisms in sorghum rhizosphere soil, while the third appears to be inhibitory. Two bioactive metabolites in soybean rhizosphere soil were identified, one apparently faciliatory and one inhibitory towards microorganisms. Multivariate analysis showed that maize-soybean and sorghum-soybean intercropping systems changed the soil micro-environment and reshaped the soil microbial community structure through interspecific root interactions in rhizosphere soil, which accelerated the deposition of available nutrients, promoted the absorption of crop nutrients, and ultimately increased crop yield.

    Responses of soil bacterial and fungal communities to precipitation in the desert steppe ecosystem of Ningxia
    Yang MI, Rong GUO, Yuan WANG, Zhan-jun WANG, Qi JIANG, Hong-qian YU, Kun MA
    2023, 32(11):  81-92.  DOI: 10.11686/cyxb2023001
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    Bacteria and fungi are the most important soil microbial groups, but their response mechanism to precipitation change in desert grassland ecosystems remains unclear. We investigated the response mechanism of soil bacterial and fungal communities to long-term precipitation differences in the desert grassland of Ningxia. Four national monitoring sites with different precipitation characteristics in the desert grassland of Ningxia were selected for study. Precipitation treatments were: 231 (T0), 154 (T1), 137 (T2) and 114 mm (T3) annually. Using the IonS5TMXL high-throughput sequencing method, the differences in bacterial and fungal populations in soil under the long-term precipitation regime changes in desert grassland were investigated. It was found that bacterial and fungal operational taxonomic units (OTUs) generally showed an initial increase and then a decreasing trend with progressive decrease in precipitation along the treatment gradient. Proteobacteria, Actinobacteria and Acidobacteria were the predominant bacteria soil communities at the phylum level, and Ascomycota and Basidiomycota were the predominant fungal communities in this desert grassland. Differences in precipitation significantly affected the relative abundance of dominant bacteria (Proteobacteria, Actinobacteria and Acidobacteria) and non-dominant bacteria (Verrucomicrobia) at the phylum level, and also the relative abundance of Glomeromycota (P<0.05), but did not significantly affect the relative abundance of the predominant fungal communities (Ascomycota, Basidiomycota). Compared with treatment T0, the richness indexes (Chao1 index and ACE index) of bacteria and fungi were significantly higher in treatments T1, T2 and T3, and the Shannon-Wiener diversity index of bacteria was also significantly higher. However, the Shannon-Wiener diversity index of fungi was not significantly affected. The total nitrogen and available phosphorus were the most important environmental factors driving bacterial community change, and total phosphorus and soil organic matter (OM) were the driving environmental factors for fungal community change. Plant factors (plant biomass) and soil factors (soil nutrients) were considered together and their role in the regulation processes of bacterial and fungal communities in soil under the influence of precipitation was examined. The soil factors played an important role in controlling the different responses of the soil bacterial and fungal communities. So, we concluded that the soil bacterial and fungal communities have different response mechanisms to changes in biotic and abiotic factors caused by precipitation differences. These different response mechanisms underpin the different adaptation strategies of bacterial and fungal communities in desert grassland.

    Changes in soil bacterial carbon source utilization and soil extracellular enzyme activity after artificial planting of Caragana korshinskii at different densities on the desert steppe
    Rong-xia SU, Yan-ping MA, Hong-mei WANG, Ya-nan ZHAO, Zhi-li LI
    2023, 32(11):  93-105.  DOI: 10.11686/cyxb2023020
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    The aim of this study was to explore the effects of shrub introduction on the functional diversity of soil bacterial communities and soil extracellular enzyme activity on the desert steppe. Field studies were conducted in areas where Caragana korshinskii has been introduced with different planting intervals into grassland on the desert steppe of eastern Ningxia. We investigated the characteristics of soil bacterial carbon utilization in fenced grassland and shrublands planted with C. korshinskii at a range of intervals (40, 6 and 2 m) using the Biolog-ECO method. We found that the soil organic carbon and microbial biomass carbon contents were not significantly higher in shrublands than in enclosed desert grassland, while soil total carbon content was significantly higher (by 26.65%-43.47%) in shrublands than in enclosed grassland. The activities of all measured soil extracellular enzymes, except β-1,4-N-acetylamino glucosidase, were lower in shrublands than in enclosed grassland, but there was no significant difference in their activities among the different planting intervals. Further analyses including a principal component analysis revealed significant differences in the metabolic functions of soil bacterial communities among sites. The relative utilization of carbon sources decreased by 49.2% with the increase of shrub introduction intervals in the last 30 years. The utilization of carbon sources by soil bacteria and the functional diversity of soil bacterial communities were significantly higher in shrublands than in enclosed grassland. The Shannon-Wiener richness index, Shannon’s evenness index, and Simpson’s dominance index of soil bacterial communities increased with increasing planting intervals of C. korshinskii. Soil bacteria in grassland mainly utilized amino acids, polymers, and other types of compounds as carbon sources, but those in shrublands stably utilized polymers and amino acids as carbon sources.

    Differences in traits related to lodging resistance among oat genotypes
    Ming NAN, Xing-rong WANG, Jing LI, Yan-ming LIU, Cheng-jun ZHANG, Ji-kuan CHAI, Gui-qin ZHAO
    2023, 32(11):  106-118.  DOI: 10.11686/cyxb2023010
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    Lodging is one of the main factors affecting oat yield and quality. Screening to identify the morphological traits related to the lodging resistance of oats and the construction of an evaluation system are important steps towards identifying and utilizing superior oat germplasm. In this study, we evaluated and compared 18 morphological characters of the root, stem, and ear of 20 oat genotypes. The morphological indexes closely related to lodging resistance were screened by correlation analysis and principal component analysis (PCA). An evaluation system for lodging resistance based on morphological indexes of oat was constructed by a step linear regression method. This system provides the basis for breeding lodging-resistant oat varieties. Some traits varied widely among the different oat genotypes. Morphological traits showed extremely significant differences among genotypes (P<0.01). The root, stem, and ear morphological traits of the same oat genotypes differed significantly between the two test sites (P<0.05) and there was a significant interaction effect between the genotype of each morphological character and the test environment (P<0.05). The average coefficient of variation of the actual lodging rate was 81.17%, and there was a large amount of genetic variation. Among all of the morphological traits, barycenter height had the smallest coefficient of variation (7.91%). Stem strength, center of gravity height, basal internode stem length, and root volume were closely related to lodging resistance, and were the key indexes affecting oat lodging resistance. The PCA identified three key factors representing 85.082% of the information provided by 11 morphological traits. Systematic clustering was carried out based on 11 traits including root volume, stem base length (1st and 2nd internode lengths), stem strength, and ear morphology. Three morphological trait cluster groups, seven cluster subgroups, and three genotype cluster groups were obtained. The scores from the cluster heat map, two-dimensional PCA map, and function model were basically consistent with the degree of lodging of different genotypes and the actual lodging rate, and these relationships were mutually verified. A linear regression model based on the relationship between morphological indexes and the lodging resistance of various cultivars was developed to predict the lodging resistance of oat varieties. A small root system, high center of gravity, long internodes at the base, and a slim stem were identified as factors related to lodging. A large root volume, short and thick base segments, thick stalk walls, and high stalk strength were identified as factors related to lodging resistance. Our results show that the actual lodging rate, root volume, center of gravity height, stem base length (1st and 2nd internode lengths), stem diameter, and stem strength are useful characters for evaluating the lodging resistance of oats. The lodging resistance evaluation system is relatively reliable and can be used to evaluate the lodging resistance of oat varieties and lines in the field.

    Effect of feed level of oat hay on average daily gain, blood physiological and biochemical indexes, and rumen fermentation parameters in yaks
    Dao zhi cai rang WU, Cheng-fang PEI, Zhi-yuan MA, Hong-shan LIU, Xu-liang CAO, Hu LIU, Jian-wei ZHOU
    2023, 32(11):  119-129.  DOI: 10.11686/cyxb2022494
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    This study was conducted to investigate the response of average daily gain, blood physiological, biochemical indexes and rumen fermentation parameters to different feeding levels of pelleted oat hay in yaks, in order to provide technical data for scientifically informed yak farming on the Qinghai-Tibetan Plateau. Six healthy castrated yaks [(203.0±6.0) kg], aged 3.5 years old, were selected for the experiment which used a 4×4 Latin square design. The two additional animals were considered as biological replicates for one of the Latin square sequences. Voluntary intake of each animal was determined in a 14-day pretrial test, then oat hay feeding levels were set at 0.45, 0.60, 0.75 and 0.90 VI (voluntary intake). It was found that: 1) The average daily gain (ADG) of the yaks increased linearly (P<0.001) with increase in oat hay feeding level. A regression equation ADG (g·day-1)=973 X-513 (R2=0.727) was determined where X denotes metabolic energy allowance [MJ·kg-1·BW-0.75·d-1] established based on the ADG (Y) and metabolic energy (ME, X) intakes in yaks, and the estimated body maintenance ME requirement was 0.527 MJ·kg-1·BW-0.75·d-1. 2) As the feed level of oat hay increased, the population of red blood cells was decreased linearly (P<0.05), while the population of platelets was increased linearly (P=0.05), and the other blood physiological indexes were not affected by feeding level treatments (P≥0.10). 3) With increase in the feed level of oat hay, serum concentrations of urea nitrogen and growth hormone increased linearly (P<0.05) while glucose concentration decreased linearly (P<0.05). Meanwhile, serum insulin concentration tended to increase (P=0.091) with feed intakes whereas β-hydroxybutyric acid level tended to decrease (P=0.063). 4) The concentrations of ruminal total volatile fatty acid, acetate and butyrate increased linearly (P<0.05) with increased oat hay feeding level in yaks, and propionate concentration tended to increase (P=0.093), accompanied with a decrease of ruminal pH (P<0.05). The above results showed that both blood physiological and biochemical indexes and rumen fermentation parameters in yaks were somewhat affected by the feeding level of forages, which in turn ultimately determined the growth performance. According to the maintenance ME requirement of yaks estimated in present study, the dry matter intake of oat hay should be greater than 50.7 g·kg-1·BW-0.75·d-1 in order for the animals to maintain their body weight or gain weight.

    Distribution of sodium, potassium, and magnesium in 70-100 kg yak calves and the growth requirements for these nutrients
    Jia-yu DUAN, Bo ZHANG, Jun CAO, Shu-jie LIU, Zhan-hong CUI
    2023, 32(11):  130-139.  DOI: 10.11686/cyxb2023030
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    The aim of this work was to explore the distribution of sodium, potassium, and magnesium in 70-100 kg yak calves, and to determine the growth requirements for these minerals in these calves. A 75-day experiment consisting of a 15-day pre-feeding period and a 60-day positive-feeding period was conducted from November 2020 to January 2021. Twenty-one yak calves (male) with good body condition and similar body weight [average, (60.44±4.59) kg] were selected and randomly divided into three groups; The initial slaughter group (BL group), the middle slaughter group (M group), and the final slaughter group (F group). The three groups of calves were fed the same kind of feed, ad libitum, during the pre-feeding period of 15 days and the 60-day positive feeding period. The calves in the BL, M, and F groups were slaughtered at the end of the pre-feeding period, mid-way through the positive-feeding period, and at the end of the positive-feeding period, respectively. At slaughter, the average weight of calves in the BL, M, and F groups was 73.61, 81.96, and 96.53 kg, respectively. After slaughtering the calves, tissue samples were collected and weighed. The contents of sodium, potassium, and magnesium in the tissues were determined, and the distribution patterns of these minerals in the calf body were analyzed. A mathematical model was established to predict the growth requirements for these minerals in yak calves. It was found that: 1) The meat weight, viscera (including blood) weight, tare weight, and gross weight all increased significantly (P<0.05) with increasing body weight of yak calves. The bone weight and fat weight were significantly higher in the F group than in the other two groups (P<0.05). During the experiment, the bone growth rate decreased, while the muscle growth rate showed an increasing trend. 2) More potassium was distributed in muscle than in fat; And more sodium and magnesium were distributed in bones than in other tissues. A small amount of magnesium was distributed in fat and hair. 3) The sodium and potassium contents were higher in the F group than in the other two groups. On an empty (fasting) body weight (EBW) basis, the formulae approximating the nutrient requirements for gain (NRG) for sodium, potassium and magnesium were, respectively NRG=1.0125×EBW-0.0807NRG=0.8810×EBW0.2650, and NRG=0.04567×EBW0.5209. These results show that, for 70-100 kg yak calves, the NRGs for sodium, potassium, and magnesium are 0.73-0.74 g·kg-1 EBW, 2.49-2.65 g·kg-1 EBW, and 0.35-0.40 g·kg-1 EBW, respectively.

    Meat quality and nutritional indexes of three muscles of the F1 generation of crossbred sheep (Suffolk×river valley-type Tibetan) raised under grazing conditions
    Meng-jun LIU, Yue REN
    2023, 32(11):  140-154.  DOI: 10.11686/cyxb2023008
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    The aim of this study was to investigate the quality and nutritional characteristics of meat from the F1 generation of crossbred Suffolk×river valley-type Tibetan sheep. The meat color, cooked meat rate, pH, conventional nutritional indexes, and contents of minerals, cholesterol, vitamin A, vitamin E, vitamin B1, vitamin B2, amino acids, and fatty acids in three muscles (longissimus dorsi, brachial triceps, and biceps femoris) were determined. It was found that there were some differences in meat quality among three different muscles of the crossbred F1 generation, mainly in meat color and the contents of crude protein, zinc, amino acids, and fatty acids. In CIE-Lab color analyses, the meat color L* (lightness) value was significantly higher for the longissimus dorsi and triceps brachii than for the biceps femoris (P<0.05), the meat color a* value was significantly higher for the longissimus dorsi and biceps femoris than for the triceps brachii (P<0.05), and the meat color b* value was significantly higher for the longissimus dorsi than for the triceps brachii and biceps femoris (P<0.05). The crude protein content was significantly higher in the longissimus dorsi than in the triceps brachii (P<0.05), and the zinc content was significantly higher in the triceps brachii than in the longissimus dorsi and biceps femoris. The glutamate content was significantly higher in the triceps brachii than in the longissimus dorsi (P<0.05), and the histidine content was significantly higher in the longissimus dorsi than in the biceps femoris and triceps brachii (P<0.05). The palmitic acid content was significantly higher in the longissimus dorsi and biceps femoris than in the triceps brachii (P<0.05). The arachidonic acid content was significantly higher in the brachialis triceps than in the longissimus dorsi (P<0.05). In conclusion, the fresh taste amino acid content was slightly higher in the brachial triceps than in the longissimus dorsi, and the quality of the longissimus dorsi was better than that of the brachial triceps and biceps femoris. The nutritional values of the three muscles were similar, and all of them met the human healthy diet standards.

    Effects of different additives on the fermentation quality of bamboo shoot shells and on rumen microorganisms in Hu Sheep
    Juan-qing QIN, Hao-qian DANG, Hua-yun JIN, Yu-kang GUO, Fu ZHANG, Qing-hua LIU
    2023, 32(11):  155-167.  DOI: 10.11686/cyxb2023016
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    The aim of this experiment was to investigate the effects of different additives on the fermentation quality of bamboo shoot shells, and to investigate the effects of the various silage products on rumen microorganisms of Hu Sheep. Formic acid, cellulase, and effective microorganisms were added alone or in combination and their effects on the fermentation of bamboo shoot shells in a small-tank silage system were determined. The experiment consisted of a control (CK, no additives) and seven treatment groups: The FA group (formic acid), CE group (cellulase), EM group (effective microorganisms), FC group (formic acid+cellulase), CM group (cellulase+effective microorganisms), FM group (formic acid+effective microorganisms), and FCM group (formic acid+cellulase+effective microorganisms). The nutrient composition and fermentation quality of the silage in each treatment and in CK were measured after 80 days of fermentation. On the basis of these analyses, the optimal groups, FM and FCM, were selected for a feeding experiment with Hu Sheep. In the feeding experiment, 30 healthy, disease-free male Hu Sheep with similar body weight [(16.68±1.05) kg] were selected and randomly divided into three groups, with 10 sheep per group. It was found that: 1) The sensory quality of silage was good in the FM and FCM groups, poor in CK and the CM group, and average in all the other groups. 2) The dry matter (DM) contents were 32.14%, 32.37%, 32.30%, 33.96%, and 34.15% in the FA, EM, FC, FM, and FCM groups, respectively, while the crude protein (CP) contents were 9.31%, 9.64%, 9.80%, 10.82%, and 11.18%, respectively. The DM and CP contents were significantly higher in all treatment groups than in the control (DM, 29.34%; CP, 8.57%) (P<0.05). The neutral detergent fiber content was significantly lower (P<0.05) in FCM silage (35.06%) than in FM silage (37.48%); While acid detergent fiber contents were significantly lower (P<0.05) in FM silage (27.45%) and FCM silage (26.61%) than in CK silage (34.39%). 3) The pH was significantly lower (P<0.05) in FA and FCM silages (3.75 and 3.74, respectively) than in FM, FC, and CK silages (3.94, 3.86, and 4.15, respectively). The lactate contents in FA, EM, FC, FM, and FCM silages were 8.44%, 8.33%, 9.25%, 10.83%, and 11.23%, respectively, significantly higher than in CK silage (7.90%) (P<0.05), and significantly higher in FM and FCM silages than in FA, EM, and FC silages (P<0.05). The ammonia nitrogen/total nitrogen (AN/TN) levels in the FA, FC, FM, and FCM silages were 5.51, 6.05, 5.97, and 5.45, respectively, significantly lower than in CK silage (7.24) (P<0.05). 4) The average length of rumen papilla in Hu sheep fed with FCM and FM silages was 1.79 and 1.72 mm, respectively, significantly longer than that in Hu sheep fed with CK silage (1.28 mm) (P<0.05). 5) The Chao1 index value of microbial communities in the rumen of sheep fed with FCM and FM silages was 3007.31 and 2897.70, respectively, significantly higher than that of the microbial community in the rumen of sheep fed with CK silage (2456.21) (P<0.05). 6) The relative abundance of Ruminococcaceae in the rumen was lower in sheep fed with CK and FCM silages (17.12% and 19.12%, respectively) than in those fed with FM silage (20.98%), but the difference was not significant (P>0.05). The relative abundance of BS11 in the rumen was higher in the sheep fed with FM silage (6.44%) and FCM silage (6.12%) than in those fed with CK silage (1.84%), but the difference was not significant (P>0.05). In conclusion, silages made from bamboo shoot shells differ in their sensory quality, fermentation characteristics, and nutritional quality and can promote the rumen morphological development of Hu sheep, but their effects on the composition and structure of rumen microbial communities are not obvious.

    Salt tolerance identification and evaluation of a population of wild soybean SP1 mutants at the seedling stage
    Zong-chang XU, Xue-li LU, Yun-chong WEI, Chen MENG, Meng-chao ZHANG, Yuan-yang ZHANG, Meng WANG, Ju-ying WANG, Cheng-sheng ZHANG, Yi-qiang LI
    2023, 32(11):  168-178.  DOI: 10.11686/cyxb2023012
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    Soil salinity is one of the major issues affecting agricultural production in many countries, including China. To identify and obtain highly salt-tolerant wild soybean (Glycine soja) genotypes for cultivation in saline-alkali regions, the salt tolerance of spaceflight-mutated wild soybean materials was evaluated. In total, 129 SP1 samples of wild soybean that had undergone spaceflight mutagenesis were treated with 150 mmol·L-1 NaCl and then 11 indexes were measured. The indexes included plant height, hypocotyl length, hypocotyl diameter (stem diameter), chlorophyll content, single leaf dry and fresh weight, and leaf area. The salt tolerance of wild soybean mutant materials was assessed at the seedling stage using multivariate statistical analyses including principal component analysis and cluster analysis. There were significant variations in agronomic traits among the wild soybean mutant materials. The coefficient of variation of the various traits ranged from 6.45% to 51.78%. The most significant correlations among the 11 traits were found between plant height at 7 days and 21 days and leaf area. Based on the first five principal component scores, the D value representing a comprehensive evaluation index was determined. Ten highly salt-tolerant mutant materials, 53 salt-tolerant materials, 63 intermediate salt-tolerant materials, and three salt-sensitive materials were screened based on the D value. Peroxidase activity was identified as a crucial factor in the salt tolerance of wild soybean mutant materials. These salt-resistant materials are promising foundational resources for breeding new, salt-tolerant soybean varieties.

    Response of germination and seedling survival of two shrubs to simulated dry-wet treatments
    Wen-jie QU, Wen-zhi ZHAO, Lei WANG, Jian-jun QU, Xin-guo YANG
    2023, 32(11):  179-187.  DOI: 10.11686/cyxb2022493
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    Caragana korshinskii and Corethrodendron scoparium are two typical xeriscape shrubs widely used in ecological construction in arid and semi-arid areas. Differences in precipitation intensity and interval profoundly affect germination and seedling survival, and the two processes are key factors limiting vegetation natural regeneration. In this study, the effects of dry-wet treatments on C. korshinskii and C. scoparium germination and seedling survival were examined using standard procedures. It was found that, compared to CK, only C. korshinskii germination was significantly affected by dry-wet treatments. The best treatments increased germination of C. korshinskii at day 1 by 27.99% (P<0.05), and of C. scoparium at day 2 by 46.88% (P<0.05). Compared to C. korshinskii, the revival rate of young C. scoparium seedlings after rehydration was higher. Yong seedlings of C. korshinskii and C. scoparium with root length of 0-0.2 cm desiccated up to 45 days revived after rehydration. The points of no return of young C. korshinskii and C. scoparium seedlings of 0.5-1.0 cm root length were 21 and 45 days, respectively. At a root length of 1.0-1.5 cm, the points of no return were 7 and 14 days, respectively. In summary, the hydration/dehydration treatments were beneficial to germination, but the tolerance to desiccation decreased with the growth of seedlings. In general, the adaptation of C. scoparium to desiccation was stronger than that of C. korshinskii. We suggest that more use of shrubs such as C. scoparium can be made to fix sand as part of response to climate change in the study area, so as to improve the relative stability of artificial vegetation.

    The Leymus chinensis industry in China needs to be urgently revitalized
    Ya TAO, Li-jun XU, Feng LI, Wen-long LI, Qi-zhong SUN, Chang XU, Ke-jian LIN
    2023, 32(11):  188-198.  DOI: 10.11686/cyxb2023003
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    Leymus chinensis is a famous Poaceous forage in China, with a history of breeding and improvement programs spanning more than 70 years. It plays an important role in ecological construction and animal husbandry production. As at the beginning of the 21st century, L. chinensis had played an important role in the development of the modern dairy industry. However, as China enters the current new development period, with the alfalfa and forage oats industries booming, the L. chinensis industry is in a depressed state. However, China’s alfalfa and forage oats industries are facing many difficult challenges (such as land and water shortages); Hence, it is particularly important and urgent to examine the new advantages and new meanings of revitalizing the L. chinensis industry with new ideas, new perspectives and new technologies. Compared with forage oats, L. chinensis has obvious advantages in both stress resistance eco-biology and cultivation requirements. However, due to the limited policy support for the L. chinensis industry, its development lags behind that of the alfalfa and forage oat industries. In the future, policy support for the L. chinensis industry and scientific and technological innovation should be strengthened. Major breakthroughs should be pursued in the “three-low” problems (low heading rate, low seed setting rate and low germination rate) and seed yield improvement. Greater progress should be an imperative in the key core technologies of the whole L. chinensis industrial chain, such as large-scale planting, intelligent management, water-saving irrigation, mechanized operation, standardized processing, specialized production and competitive cost of the forage to users. Significant effort should be expended to expand the planting area, improve the quality and increase the yield of L. chinensis, as well as to realize the win-win situation for both ecology and economy. Full play should be given to the ecological and biological advantages and cultural biological characteristics of L. chinensis, by developing its use on marginal land, so as to break resource constraints, cultivate leading enterprises, establish a digital trading platform, open up the sales channels of products, and create well-known L. chinensis products and brands.

    Advances in technologies to detect the seed vigor of grasses
    Hui-fang YAN, Yu-ting NIE, Li-li CONG, Zhao ZHANG, Kai-lun CUI, Yan-zhen LYU, Mao-feng CHAI
    2023, 32(11):  199-211.  DOI: 10.11686/cyxb2023043
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    Grass seeds are the fundamental materials for creating artificial grassland, improving the ecological environment of degraded grassland, and ensuring the production potential of grassland for grazing animals. Seed vigor directly affects the production and development of the grassland industry. In China and abroad, seed vigor detection technology has always been an important focus of seed science and technology. However, few studies have focused on the application of seed vigor detection technologies to grass seeds. At present, seed vigor detection technology in grasses includes destructive and non-destructive detection. In this review, we describe recent advances in seed vigor detection in grasses. We introduce destructive detection technologies based on seed germination behavior and physiological and biochemical characteristics, and non-destructive detection technologies based on optical characteristics and appearance characteristics. Additionally, we discuss trends in the development and potential applications of non-destructive technologies to assess seed vigor in grasses. The results of such analyses can provide a reference for the accurate determination of grass seed vigor.

    Effect of abnormal precipitation on the diversity of plant functional groups on the desert steppe under different stocking rates
    Jiang-wen LI, Jing-hong PEI, Guo-dong HAN, Bang-yin HE, Cai LI
    2023, 32(11):  212-222.  DOI: 10.11686/cyxb2023033
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    Plant functional groups play an important role in grassland ecosystem functions. There are various classification standards for functional groups, but the use of functional groups as a plant classification method still lacks theoretical support. In the present study, the Stipa breviflora desert steppe in Inner Mongolia was studied to detect differences in community-weighted mean traits, species richness, and functional group diversity under different stocking rates, with an overall aim to explain the dynamic changes in plant functional groups. It was found that increased precipitation significantly increased the values of some community-weighted mean traits (plant height, width of the clump crown, density, individual biomass, leaf area, and specific leaf area) and significantly decreased the values of others (leaf biomass and individual leaf mass) (P<0.05). The values of the community-weighted mean traits of plant height, width of clump crown, and stem biomass decreased significantly with increasing stocking rate, and heavy grazing resulted in a significant reduction in the community-weighted mean trait of density (P<0.05). Based on plant functional traits, 57 plants were divided into four plant functional groups. The diversity and species richness of plant functional groups were significantly higher in wet years than in dry years. The diversity and species richness of plant functional groups decreased significantly with increasing stocking rates (P<0.05). Overall, our results show that plants can be assigned to different functional groups according to their functional traits, and that increasing precipitation and decreasing the stocking rate can significantly increase the diversity and species richness of community functional groups. The responses of community-level plant functional traits on the desert steppe to increased precipitation and decreased stocking rate were consistent. However, the responses of individual plants with particular functional traits to different precipitation and stocking rates were not consistent. The results of this study provide a theoretical basis for further research on biodiversity conservation and ecosystem services on the desert steppe.