多粘类芽孢杆菌β-葡萄糖苷酶 bglA、 bglB和 bgl基因在大肠杆菌中的表达 |
王艳 , 马亚茹, 万学瑞, 王川, 吴润, 刘桂林, 刘原子, 吴自祥 |
Expression of β-glucosidase genes bglA, bglB, and bgl from Bacillus polymyxa in Escherichia coli |
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WANG Yan
, MA Ya-Ru, WAN Xue-Rui, WANG Chuan, WU Run, LIU Gui-Lin, LIU Yuan-Zi, WU Zi-Xiang |
| 重组质粒图谱及β-葡萄糖苷酶基因的双酶切验证 A:pET-28a:: bglA 重组质粒图谱Plasmid map of pET-28a:: bglA ;B:pET-28a:: bglB 重组质粒图谱Plasmid map of pET-28a:: bglB ;C:pET-28a:: bgl 重组质粒图谱Plasmid map of pET-28a:: bgl ;D:β-葡萄糖苷酶基因的双酶切验证β-glucosidase gene recombinant plasmid digested;M:DNA marker;1:pET-28a:: bglA 重组质粒双酶切( BamH Ⅰ和 Xho Ⅰ)pET-28a:: bglA digested by BamH Ⅰand Xho Ⅰ;2:pET-28a:: bglB 重组质粒双酶切( BamH Ⅰ和 Xho Ⅰ) pET-28a:: bglB digested by BamH Ⅰand Xho Ⅰ;3:pET-28a;4:pBluescriptⅡKS(+);5:pBluescriptⅡKS(+):: bgl 重组质粒双酶切( Sma Ⅰ和 BamH Ⅰ)pBluescriptⅡKS(+):: bgl digested by Sma Ⅰand BamH Ⅰ;6:pET-28a:: bgl 重组质粒双酶切( BamH Ⅰ和 Xho Ⅰ)pET-28a:: bgl digested by BamH Ⅰand Xho Ⅰ. |
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