多粘类芽孢杆菌β-葡萄糖苷酶 bglA、 bglB和 bgl基因在大肠杆菌中的表达 |
王艳 , 马亚茹, 万学瑞, 王川, 吴润, 刘桂林, 刘原子, 吴自祥 |
Expression of β-glucosidase genes bglA, bglB, and bgl from Bacillus polymyxa in Escherichia coli |
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WANG Yan
, MA Ya-Ru, WAN Xue-Rui, WANG Chuan, WU Run, LIU Gui-Lin, LIU Yuan-Zi, WU Zi-Xiang |
| SDS-PAGE电泳 M:蛋白Marker Protein marker;1:EA诱导前EA before induction;2:EB诱导前EB before induction;3:EA和EB混合表达诱导前EA and EB cultivation by mixed proportion 1∶1 before induction;4:EAB诱导前EAB before induction; 5:EA诱导后EA after induction; 6:EB诱导后EB after induction; 7:EA和EB混合表达诱导后EA and EB cultivation by mixed proportion 1∶1 after induction; 8:EAB诱导后EAB after induction; 9:EA纯化后 EA after purification;10:EB纯化后 EB after purification;11:EA和EB混合表达纯化后EA and EB cultivation by mixed proportion 1∶1 after purification;12:EAB纯化后EAB after purification.EA: bglA 基因连接pET-28a后转入C41感受态细胞的重组菌株Recombination strains of bglA was linked with pET-28a and expressed in Escherichia coli C41;EB: bglB 基因连接pET-28a后转入C41感受态细胞的重组菌株Recombination strains of bglB was linked with pET-28a and expressed in Escherichia coli C41;EAB: bglA , bglB 基因连接pET-28a后转入C41感受态细胞的重组菌株Recombination strains of bglA , bglB was linked with pET-28a and expressed in Escherichia coli C41. 下同 The same below. |
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