多粘类芽孢杆菌β-葡萄糖苷酶 bglA、 bglB和 bgl基因在大肠杆菌中的表达
王艳, 马亚茹, 万学瑞, 王川*, 吴润*, 刘桂林, 刘原子, 吴自祥

Expression of β-glucosidase genes bglA, bglB, and bgl from Bacillus polymyxa in Escherichia coli
WANG Yan, MA Ya-Ru, WAN Xue-Rui, WANG Chuan*, WU Run*, LIU Gui-Lin, LIU Yuan-Zi, WU Zi-Xiang
刚果红染色水解圈 1:EA上清EA supernatant;2:EB上清EB supernatant;3:EA和EB(1∶1)混合上清EA and EB 1∶1 supernatant;4:共表达上清EAB supernatant;5:EA纯化后蛋白EA protein after purification;6:EB纯化后蛋白EB protein after purification;7:EA和EB(1∶1)混合表达纯化后蛋白EA and EB 1∶1 protein after purification;8:EA破碎后EA after breaking;9:EB破碎后EB after breaking;10:EA和EB(1∶1)混合表达破碎后EA and EB 1∶1 after breaking;11:共表达破碎后EAB after breaking;12:共表达纯化后蛋白EAB protein after purification;13:BglA和BglB(1∶1)BglA and BglB 1∶1;14:多粘类芽孢杆菌 B. polymyxa ;15:BglA和BglB(2∶1)BglA and BglB 2∶1;16:BglA和BglB(1∶2)BglA and BglB 1∶2.