PEG介导的柳枝稷叶肉细胞原生质体瞬时表达体系的建立
祁泽文1, 孙鑫博2, 樊波3, 张雪1, 袁建波1, 韩烈保1,*

Establishment of a gene transient expression system mediated by polyethylene glycol in switchgrass ( Panicum virgatum) mesophyll protoplasts
QI Ze-Wen1, SUN Xin-Bo2, FAN Bo3, ZHANG Xue1, YUAN Jian-Bo1, HAN Lie-Bao1,*
载体PCR扩增 MYB指的是含有MYB结构域的转录因子;LN指的是双元表达载体; marker为DL2000的marker;a:从植物cDNA中扩增得到的 OsMYB 103基因, 大小为1 kb左右;b:载体LN的双酶切胶图;c:LN-103(LN- OsMYB 103)的双酶切图, 载体大小完全正确。MYB refers to the transcription factor; LN refers to the binary expression pEZR; marker:DL2000 marker; a:The OsMYB 103 gene, which is about 1 kb in size, was amplified from cDNA;b: The double enzyme map of pEZR-LN;c:The double enzyme map of LN-103 and the pEZR size is correct.