大豆 GmCYS20基因在百脉根共生结瘤过程中的功能研究
柯丹霞1,2,*, 彭昆鹏1, 张孟珂1, 贾妍1

Function of the soybean GmCYS20 gene in symbiotic nodulation of Lotus japonicus
KE Dan-xia1,2,*, PENG Kun-peng1, ZHANG Meng-ke1, JIA Yan1
植物表达载体的构建及阳性毛根的鉴定 A: p1302G-GmCYS20的酶切鉴定 Identification of p1302G-GmCYS20 by enzyme digestion; M: DL2000 DNA marker; 1: p1302G-GmCYS20的EcoR I 和 Sma I 双酶切图p1302G-GmCYS20 digested with EcoR I and Sma I; B:毛根根尖的GUS染色图,箭头所示为不显蓝色的阴性毛根 GUS staining of the hairy root apex. The arrowhead is shown as a negative hairy root apex that does not show blue; C:阳性毛根接种根瘤菌4周后的GUS染色图 GUS staining of the positive hairy roots after inoculation with rhizobium for 4 weeks.