不同早熟禾品种对干旱胁迫的生理响应及抗旱性评价

doi:10.11686/cyxb20140427

摘要/Abstract

摘要： 为鉴定评价早熟禾幼苗抗旱性,将14个早熟禾品种的种子经消毒后播种在塑料花盆中,出苗后间苗,2~3个真叶定株,每盆留生长一致、分布均匀的幼苗15株,定期浇水,以保证幼苗的正常生长。待生长到3~4个真叶时进行干旱处理,依据Michel公式计算不同水势所需的PEG-6000的量,然后加入PEG-6000溶液进行干旱处理,处理的水势梯度为0 MPa(即为对照),-0.3,-0.6,-0.9和-1.2 MPa。处理后每2 d进行称重补水以维持溶液浓度。设干旱处理与对照(正常浇水)两组,对14个早熟禾品种幼苗进行模拟干旱胁迫,4次重复。测定叶绿素(CHL)含量、丙二醛(MDA)含量、脯氨酸(Pro)含量、过氧化物酶(POD)以及超氧化物歧化酶(SOD)活性等生理生化指标。结果表明,随着干旱胁迫的加剧,早熟禾幼苗叶片CHL含量呈下降趋势;Pro含量、MDA含量、SOD活性总体呈上升趋势;POD活性总体呈先降再升后降的趋势;利用隶属函数分析得出14个早熟禾品种的抗旱性强弱顺序依次为:3号恩托佩草地早熟禾>10号细叶早熟禾>1号优异草地早熟禾>2号斗士草地早熟禾=9号蓝钻草地早熟禾>7号草地早熟禾>11号草地早熟禾>4号草地早熟禾>5号瓦巴斯草地早熟禾>8号早熟禾=13号肯塔基草地早熟禾>14号草地早熟禾>6号莫诺波利草地早熟禾=12号菲尔津草地早熟禾。

Abstract: To compare the drought resistance of bluegrass seedlings,the 14 varieties of Kentucky bluegrass seed were sowing after disinfection in the plastic pots,then thinned after emergence and fixed strains at 2 to 3 true leaf,left 15 uniform growth and distribution plants in every pot and regularly watering to ensure the normal growth of the seedlings. Drought stress trails were identified at 2 to 3 true leaf period by adding the PEG-6000 according to the Michel formula which are: 0 MPa (control),-0.3,-0.6,-0.9,-1.2 MPa in the study. Then supplement water every two days to maintain the concentration. Two groups of drought treatment and control (normal watering) were set to simulate drought stress of 14 bluegrass cultivars and four replications were set by treatment. The physiological and biochemical index,such as chlorophyll (CHL),malondialdehyde (MDA) content,proline (Pro) content,peroxidase (POD) and superoxide dismutase (SOD) activity were measured and analyzed. The content of CHL was decreased; and the content of the Pro,MDA and SOD showed upward trend; the POD activity increased at first and then decreased and finally increase again in the bluegrass seedlings; Drought resistance of 14 bluegrass varieties were obtained by subordinate function the order is: The No.3 Storpy>No.10 Narrow leaf bluegrass>No.1 Meri>No.2 Geronimoi=No.9 Blue apphire>No.7 Kentucky bluegrass>No.11 Kentucky bluegrass>No.4 Kentucky bluegrass>No.5 Wabash>No.8 Bluegrass=No.13 Kentucky>No.14 Kentucky bluegrass>No.6 Monopoly=No.12 Fylking.

中图分类号:

S543⁺.903.4

郭郁频，米福贵，闫利军，任永霞，吕世杰，伏兵哲. 不同早熟禾品种对干旱胁迫的生理响应及抗旱性评价[J]. 草业学报, 2014, 23(4): 220-228.

GUO Yu-pin,MI Fu-gui,YAN Li-jun,REN Yong-xia,LV Shi-jie,FU Bing-zhe. Physiological response to drought stresses and drought resistances evaluation of different Kentucky bluegrass varieties[J]. Acta Prataculturae Sinica, 2014, 23(4): 220-228.

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