4种燕麦对NaCl胁迫的生理响应及耐盐性评价

doi:10.11686/cyxb20150122

草业学报 ›› 2015, Vol. 24 ›› Issue (1): 183-189.DOI: 10.11686/cyxb20150122

4种燕麦对NaCl胁迫的生理响应及耐盐性评价

刘凤歧^{1, 2}, 刘杰淋², 朱瑞芬², 张悦¹, 郭勇³, 韩贵清², 唐凤兰^{2*, *}

1.哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨150025;
2.黑龙江省农业科学院草业研究所,黑龙江哈尔滨 150086;
3.佳木斯大学生命科学学院,黑龙江佳木斯 154007

收稿日期:2014-06-12 出版日期:2015-01-20 发布日期:2015-01-20
通讯作者: nkypzk@163.com
作者简介:刘凤歧(1982-),男,黑龙江克山人,助理研究员。E-mail:l_fq2003@163.com
基金资助:
黑龙江省自然科学基金项目(C201313)和哈尔滨市青年后备人才基金项目(2014RFQYJ059)资助

Physiological responses and tolerance of four oat varieties to salt stress

LIU Fengqi^{1, 2}, LIU Jielin², ZHU Ruifen², ZHANG Yue¹, GUO Yong³, HAN Guiqing², TANG Fenglan^{2, *}

1.College of Life Science and Technology, Harbin Normal University, Harbin 150025, China;
2.Institute of Pratacultural Science, Heilongjiang Academy of Agricultural Science, Harbin 150086, China;
3.College of Life Science, Jiamusi University, Jiamusi 154007, China

Received:2014-06-12 Online:2015-01-20 Published:2015-01-20

摘要/Abstract

摘要： 为研究4份燕麦坝莜一号、白燕2号、白燕6号和白燕7号的耐盐程度及其在盐胁迫处理下的生理反应规律,在水培条件下,分别用0,34.2,68.4,102.6,136.8,171.0 mmol/L浓度NaCl溶液模拟盐胁迫处理4种燕麦种子,观察记录各处理的盐害情况,分别测定了胁迫后的种子发芽势、发芽率、株高和根长,以及室温培养的燕麦幼苗叶片过氧化物酶(POD)、超氧化物歧化酶(SOD)和可溶性蛋白含量,分析了不同胁迫程度下各生理指标的变化情况,并且对4种燕麦的耐盐性进行了综合评价。结果表明,NaCl胁迫对4种燕麦种子的发芽势和发芽率均产生显著影响,对株高和根长产生了抑制作用,且均随盐浓度的增大呈逐渐降低的趋势;燕麦幼苗叶片POD活性、SOD活性、可溶性蛋白含量均在102.6 mmol/L 盐浓度下达到最大值,而且都随盐浓度的增大呈先升高后降低的趋势,表明燕麦具有较高的耐盐能力,在盐胁迫下可采取自我保护机制以适应盐胁迫,4种燕麦的耐盐阈值为102.6 mmol/L盐浓度。综合评价,白燕6号耐盐性较好,白燕2号具有高盐耐受性的潜力,这两个燕麦品种可以为耐盐基因挖掘及燕麦耐盐品种的选育提供材料。

Abstract: This paper reports on a study of the adaptability of oats (Avena sativa) to salt stress. In a hydroponic experiment, the physiological responses of four oat varieties (Bayou-1, Baiyan-2, Baiyan-6 and Baiyan-7) were investigated using six concentrations (0, 34.2, 68.4, 102.6, 136.8 and 171.0 mmol/L) of sodium chloride during seed germination and seedling stages. Seed germinating potential, germinating percentage, seedling height and root length were recorded. The activities of superoxide dismutase (SOD), peroxidase (POD) and the soluble protein content in the leaves of seedlings were also measured. Salt stress had a significant impact on germinating potential and percentage. It also suppressed seedling height and root length. All four parameters decreased with increases in Na⁺ concentration. The activities of SOD and POD and leaf soluble protein content all increased at lower levels of Na⁺ concentration and then declined, peaking at 102.6 mmol/L NaCl. This study shows that oats have self-protecting mechanisms that adapt to salinity stress. The threshold of salt tolerance was 102.6 mmol/L NaCl. Of the four varieties, Baiyan-6 showed the most tolerance, while Baiyan-2 also revealed good potential. Both varieties could be used as candidates for selecting and breeding better genotypes of salt tolerant oats.

刘凤歧, 刘杰淋, 朱瑞芬, 张悦, 郭勇, 韩贵清, 唐凤兰. 4种燕麦对NaCl胁迫的生理响应及耐盐性评价[J]. 草业学报, 2015, 24(1): 183-189.

LIU Fengqi, LIU Jielin, ZHU Ruifen, ZHANG Yue, GUO Yong, HAN Guiqing, TANG Fenglan. Physiological responses and tolerance of four oat varieties to salt stress[J]. Acta Prataculturae Sinica, 2015, 24(1): 183-189.

参考文献

[1] Xiao Q, Zheng H L, Chen Y, et al . Effects of salinity on the growth and proline, soluble sugar and protein contents of Spartina alterniflora . Chinese Journal of Ecology, 2005, 24(4): 373-376.
[2] Guo H J, Hu T, Fu J M. Effects of saline sodic stress on growth and physiological responses of Lolium perenne . Acta Prataculturae Sinica, 2012, 21(1): 118-125.
[3] Fan R P, Zhou Q, Zhou B, et al . Effects of salinization stress on growth and the antioxidant system of tall fescue. Acta Prataculturae Sinica, 2012, 21(1): 112-117.
[4] Zhu J K. Plant salt tolerance. Trends Plants Science, 2001, 6(2): 66-71.
[5] Mi H L, Xu X, Ma Y Q, et al . Study on salt-tolerance of wheat varieties. Agricultural Research in the Arid Areas, 2003, 21(1): 134-138.
[6] Levitt J. Response of Plants to Environmental Stress[M]. New York: Academic Press, 1980: 300-359.
[7] Wang B, Song F B. Physiological responses and adaptive capacity of oats to saline-alkali stress. Ecology and Environment, 2006, 15(3): 625-629.
[8] Zhao X, Wang L Q, Zhou C J, et al . Effects of salt stress on germination and emergence of different winter wheat genotypes. Agricultural Research in the Arid Areas, 2005, 23(4): 108-112.
[9] Yao Z P, Meng J, Li G. Salinity tolerance identification and screening of maize inbreeds in seedling emergence stage. Acta Agriculturae Boreali-Sinica, 2007, 22(5): 27-30.
[10] Ye W W, Pang N C, Wang J J, et al . Characteristics of absorbing, accumulating and distribution of Na + under the salinity stress on cotton. Cotton Science, 2006, 18(5): 279-283.
[11] Feng Y Q, Cao Z Z, Jia Y Q, et al . Study on salt tolerance of wild black medic germplasm. Ratacultural Science, 2007, 24(5): 27-33.
[12] Zhang G, Gao H W, Wang Z, et al . Studies on screening identification indexes of salt tolerance and comprehensive evaluation at seedling stage of Elytrigia . Acta Prataculturae Sinica, 2007, 16(4): 55-61.
[13] Wu F P, Han Q F, Jia Z K. Preliminary study on the salt tolerance of four alfalfa lines in seed germination period. Pratacultural Science, 2008, 25(8): 57-62.
[14] Wang B, Song F B. The effects of saline-alkali stress on water potential, percentage of dry matter and selective absorption to K + and Na + in oats. Systemsciences and Comprehensive Studies Inagriculture, 2006, 22(2): 105-108.
[15] Wang B, Zhang J C, Song F B, et al . Physiological responses to saline-alkali in oats. Journal of Soil and Water Conservation, 2007, 21(3): 86-89.
[16] Colowick S P, Kaplan N O. Methods in Enzymology[M]. New York: Academic Press, 1955: 764-775.
[17] Giannopolitis C N, Ries S K. Superoxide dismutase: I.Occurrence in higher plant. Plant Physiology, 1977, 59(2): 309-314.
[18] Cang J, Yu L F, Wang Y Y, et al . Agronomic and biochemical characters of chlorophyll deficient mutant Hs 821 of soybean. Journal of Nuclear Agricultural Sciences, 2007, 21(1): 9-12.
[19] Zhu X Y, Wang S M, Yan S G, et al . The research progresses pant resistance salt and its mechanism of resistance genus Puccinellia . Acta Prataculturae Sinica, 1994, 3(3): 9-15.
[20] Hao Z G, Hu Z Z, Zhu X Y. The studies on salt tolerance in Puccinellia spp. Acta Prataculturae Sinica, 1994, 3(3): 27-36.
[21] Macke A J, Ungar I A. The effects of salinity on germination and early growth of Puccinellia nuttalliana . Canadian Journal of Botany, 1971, 49: 515-520.
[22] Lu Y, Lei J Q, Zeng F J, et al . Effects of salt treatments on the growth and ecophysiological characteristics of Haloxylon ammodendron . Acta Prataculturae Sinica, 2014, 23(3): 152-159.
[23] Unga I A. Halophyte seed germination. Botany Review, 1982, 44: 233-264.
[24] Salman G, Ajmal K M. Seed germination of a Halophy-tiegrass Aeluropus lagopoides . Annul of Botany, 2001, 87: 319-324.
[25] Waisel Y. Biology of Halophytes[M]. New York: Aeademie Press, 1972.
[26] McCord J M, Fridovich I.The utility of superoxide dismutase in studying free radical reactions. I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen.Journal of Biological Chemistry, 1969, 244(22): 6056-6063
[27] Fridovich I. Superoxide dismutase. Annual Review of Biochemistry, 1975, 44: 147-159.
[28] Asada K, Kanematsu S, Okada S, et al . Phylogenetic Distribution of Three Types of Superoxide Dismutase in Organisms and in Cell Organelles[M]. Amsterdam: Elsevier, 1980: 136-153.
[29] Zeng Q P, Guo Y. The stress response and system induced resistance of plants. Chemistry of Life, 1997, 17(3): 31-33.
[30] Zhang H S, Zhao G Q, Li M F, et al . Physiological responses of Pennisetum longissimum var. intermedium seedlings to PEG, low temperature and salt stress treatments. Acta Prataculturae Sinica, 2014, 23(2): 180-188.
[31] Meng Y X, Wang S H, Wang J C, et al . Influences of CoCl 2 on the growth and seedling physiological indexes of Hordeum vulgare under NaCl stress. Acta Prataculturae Sinica, 2014, 23(3): 160-166.
[1] 肖强, 郑海雷, 陈瑶, 等. 盐度对互花米草生长及脯氨酸、可溶性糖和蛋白质含量的影响. 生态学杂志, 2005, 24(4): 373-376.
[2] 郭慧娟, 胡涛, 傅金民. 苏打碱胁迫对多年生黑麦草的生理影响. 草业学报, 2012, 21(1): 118-125.
[3] 樊瑞苹, 周琴, 周波, 等. 盐胁迫对高羊茅生长及抗氧化系统的影响. 草业学报, 2012, 21(1): 112-117.
[5] 米海莉, 许兴, 马雅琴, 等. 小麦品种耐盐性的研究. 干旱地区农业研究, 2003, 21(1): 134-138.
[7] 王波, 宋凤斌. 燕麦对盐碱胁迫的反应和适应性. 生态环境, 2006, 15(3): 625-629.
[8] 赵旭, 王林权, 周春菊, 等. 盐胁迫对不同基因型冬小麦发芽和出苗的影响. 干旱地区农业研究, 2005, (4): 108-112.
[9] 姚正培, 孟君, 李冠. 玉米自交系芽苗期耐盐性的鉴定与筛选. 华北农学报, 2007, (5): 27-30.
[10] 叶武威, 庞念厂, 王俊娟, 等. 盐胁迫下棉花体内Na + 的积累、分配及耐盐机制研究. 棉花学报, 2006, (5): 279-283.
[11] 冯毓琴, 曹致中, 贾蕴琪, 等. 天蓝苜蓿野生种质的耐盐性研究. 草业科学, 2007, (5): 27-33.
[12] 张耿, 高洪文, 王赞, 等. 偃麦草属植物苗期耐盐性指标筛选及综合评价. 草业学报, 2007, 16(4): 55-61.
[13] 吴凤萍, 韩清芳, 贾志宽. 4个白花苜蓿品系种子萌发期耐盐性研究. 草业科学, 2008, (8): 57-62.
[14] 王波, 宋凤斌. 盐碱胁迫对燕麦水势、干物质积累率以及K + 、Na + 选择性吸收的影响. 农业系统科学与综合研究, 2006, 22(2): 105-108.
[15] 王波, 张金才, 宋凤斌, 等. 燕麦对盐碱胁迫的生理响应. 水土保持学报, 2007, 21(3): 86-89.
[18] 苍晶, 于龙凤, 王豫颖, 等. 大豆叶绿素缺失突变体HS 821的农艺性状和生化特性. 核农学报, 2007, 21(1): 9-12.
[19] 朱兴运, 王锁民, 阎顺国. 碱茅属植物抗盐性与抗盐机制的研究进展. 草业学报, 1994, 3(3): 9-15.
[20] 郝志刚, 胡自治, 朱兴运. 碱茅耐盐碱性的研究. 草业学报, 1994, 3(3): 27-36.
[22] 鲁艳, 雷加强, 曾凡江, 等. NaCl处理对梭梭生长及生理生态特征的影响. 草业学报, 2014, 23(3): 152-159.
[29] 曾庆平, 郭勇. 植物的逆境应答与系统抗性诱导.生命的化学, 1997, 17(3): 31-33.
[30] 张怀山, 赵桂琴, 栗孟飞, 等. 中型狼尾草幼苗对PEG、低温和盐胁迫的生理应答. 草业学报, 2014, 23(2): 180-188.
[31] 孟亚雄, 王世红, 汪军成, 等. CoCl 2 对NaCl胁迫下大麦生长及幼苗生理指标的影响. 草业学报, 2014, 23(3): 160-166.

4种燕麦对NaCl胁迫的生理响应及耐盐性评价

Physiological responses and tolerance of four oat varieties to salt stress

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics