新型饲草玉淇淋58的性状、遗传及饲草潜力

doi:10.11686/cyxb20150419

草业学报 ›› 2015, Vol. 24 ›› Issue (4): 157-163.DOI: 10.11686/cyxb20150419

新型饲草玉淇淋58的性状、遗传及饲草潜力

李华雄^1,2,程明军¹,苏月贵^1,3,李志龙^1,4,蒋维明¹,张红芬⁵,郑名敏¹,
荣廷昭¹,周树峰¹,吴元奇¹,曹墨菊¹,唐祈林^1*

1.四川农业大学玉米研究所,四川温江611130;
2.内江市农业科学院,四川内江 641000;
3.广西农业科学院,广西南宁 530007;
4.宜宾市农业科学院,四川宜宾644000;
5.石林县长湖镇农业综合服务中心,云南昆明 652203

收稿日期:2014-03-17 修回日期:2014-05-15 出版日期:2015-04-20 发布日期:2015-04-20
通讯作者: 唐祈林,E-mail:tangqilin71@163.com
作者简介:李华雄(1980-),男,四川合江人,在读博士。E-mail:498513867@qq.com
基金资助:
973项目(2014CB13870),国家自然科学基金项目(31371640)和四川省饲草育种攻关项目资助。

Chromosomal composition and forage production potential of Yuqilin 58

LI Hua-Xiong^1,2, CHENG Ming-Jun¹, SU Yue-Gui^1,3, LI Zhi-Long^1,4, JIANG Wei-Ming¹, ZHANG Hong-Fen⁵, ZHENG Ming-Min¹, RONG Ting-Zhao¹, ZHOU Shu-Feng¹, WU Yuan-Qi¹, CAO Mo-Ju¹, TANG Qi-Lin^1*

1.Maize Research Institute, Sichuan Agriculture University, Wenjiang 611130, China;
2.Neijiang Academy of Agricultural Sciences, Neijiang 641000, China;
3.Guangxi Academy of Agricultural Sciences, Nanning 530007, China;
4.Yibin Academy of Agricultural Sciences, Yibin 644000, China;
5.The Comprehensive Agricultural Service Center of Changhu Town in Shilin County, Kunming 652203, China

Received:2014-03-17 Revised:2014-05-15 Online:2015-04-20 Published:2015-04-20

摘要/Abstract

摘要： 多色基因组原位杂交(multi-color genomic in situ hybridization, McGISH)鉴定结果表明, MTPP-58有58条染色体,其中11条染色体来自玉米,28条染色体来自四倍体多年生大刍草,17条染色体来自指状摩擦禾和2条玉米与四倍体多年生大刍草易位的染色体。2012-2013年对MTPP-58的饲草产量、品质、越冬率以及繁殖特性研究表明,第1年鲜、干草产量分别为93.845 t/hm²和22.083 t/hm²,第2年鲜、干草产量分别为82.851 t/hm²和14.394 t/hm²;粗蛋白(CP)为10.48%,干茎叶比为0.71,越冬率为100%。MTPP-58下部茎干扦插成活率为51.69%,分株成活率为95.50%。多种属聚合远缘杂交创制的MTPP-58聚合了玉米、大刍草、摩擦禾的优良特性,植株根系发达,生长繁茂,分蘖和抗寒性强,生产性能优,可无性繁殖,是一种多种属间杂交创制的新型多年生饲草。

Abstract: Multi-color genomic in situ hybridization (McGISH) was employed to identify the chromosomal composition of Yuqilin 58 (MTPP-58), derived from the tri-generic hybridization of Zea mays, Zea perennis and Tripsacum dactyloides. GISH (Genomic in situ hybridization) showed that MTPP-58 is an aneuploid with 58 chromosomes; 11, 28 and 17 chromosomes came from Z. mays, Z. perennis and T. dactyloides respectively. Translocation of chromosomes between Z. mays and Z. perennis accounted for the balance. Assessment of forage yield, forage quality, over wintering rate and reproductive characteristics showed that the fresh and dry hay yield of MTPP-58 were 93.845 t/ha and 22.083 t/ha respectively, in the first year, and 82.851 t/ha and 14.394 t/ha respectively, in the second year. The crude protein content of the forage was 10.48% with a leaf/stem ratio of 0.71. Winter survival rate was 100%. MTPP-58 displayed a number of desirable characteristics including good growth, a well-developed root system, good tillering ability and good reproductive potential suggesting it has good potential for ongoing development.

李华雄,程明军,苏月贵,李志龙,蒋维明,张红芬,郑名敏,
荣廷昭,周树峰,吴元奇,曹墨菊,唐祈林. 新型饲草玉淇淋58的性状、遗传及饲草潜力[J]. 草业学报, 2015, 24(4): 157-163.

LI Hua-Xiong, CHENG Ming-Jun, SU Yue-Gui, LI Zhi-Long, JIANG Wei-Ming, ZHANG Hong-Fen, ZHENG Ming-Min, RONG Ting-Zhao, ZHOU Shu-Feng, WU Yuan-Qi, CAO Mo-Ju, TANG Qi-Lin. Chromosomal composition and forage production potential of Yuqilin 58[J]. Acta Prataculturae Sinica, 2015, 24(4): 157-163.

参考文献

Gresset S, Westermeier P, Rademacher S, et al. Stable carbon isotope discrimination is under genetic control in the C₄ species maize with several genomic regions influencing trait expression. Plant Physiology, 2014, 164(1): 131-143.
Tang Q L, Rong T Z, Song Y C, et al. Introgression of perennial teosinte genome into maize and identification of genomic in situ hybridization and microsatellite markers. Crop Science, 2005, 45(2): 717-721.
Tang Q L, Li W C, Song Y C, et al. The production and multi-color genomic in situ hybridization identification of maize-Z. perennis substituted material. Acta Genetica Sinica, 2004, 31(4): 340-344.
Ren Y, Chen R Q, Tang Q L, et al. Growth dynamics and optimum harvest period of a novel forage maize. Acta Agronmica Sinica, 2007, 33(8): 1360-1365.
Tang Q L, Wang P, Lu Y L, et al. Genetic relationship analysis of Zea species using RAPD markers. Acta Prataculturae Sinica, 2009, 18(4): 154-160.
Li D Y, Guo L Q. Research and utilization of wild relatives of maize. Journal of Maize Sciences, 2001, 9(2): 11-13.
Blakey C A, Costich D, Sokolov V, et al. Tripsacum genetics: from observations along a river to molecular genomics. Maydica, 2007, 52(1): 81-99.
Singh H, Bhardwaj B L, Gupta B K. Inheritance of forage quality and other agronomic traits in maize, teosinte and their hybrids. Crop Improvement, 2004, 31(1): 25-30.
Feng Y C, Tang Q L, Rong T Z. Comparisons on forage yield and feeding value between Zea mexicana, Yucao2 and Chuandan14-Zea mexicana Hybrid. Journal of Maize Sciences, 2011, 19(3): 68-72.
Shavrukov Y N, Sokolov V, Langridge P, et al. Interspecific hybrid, Zea mays L.×Tripsacum dactyloides L., a new fodder crop with large silage biomass production under abiotic stresses: Proceedings of the 13th Australian Agronomy Conference. Perth: Australian Centre for Plant Functional Genomics, 2006.
Kindiger B, Sokolov V, Khatypova I V. Evaluation of apomictic reproduction in a set of 39 chromosome maize-Tripsacum backcross hybrids. Crop Science, 1996, 36(5): 1108-1113.
Su Y G. Molecular Cytogenetic Study of the Tri-species Hybrid Involving Maize, Tripsacum dactyloides L. and Zea perennis. Yaan: Sichuan Agricultrual University, 2009.
Zhang L Y. Feed Analysis and Feed Quality Detection Technology. Beijing: China Agricultural University Press, 2002: 45-80.
Yang X Y, Cai Y, Fu J, et al. Karyotypes of maize and its relatives-teosinte. Scientia Agricultura Sinica, 2011, 44(7): 1307-1314.
Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 1980, 8(19): 4321-4326.
Tang Q L, Rong T Z. Study on the relationships of maize Inbred 48-2, wild maize and its hybrid (48-2×wild maize) by isoperoxidase electrophoretogram. Journal of Sichuan Agricultural University, 2003,21(1): 10-17.
Zhang H Q, Zhou Y H, Zheng Y L, et al. Morphology and cytology of intergeneric hybrids between Hystrix duthiei ssp. longearistata and Psathyrostachys huashanica (Poaceae: Triticeae). Acta Phytotaxonomica Sinica, 2002, 40(5): 421-427.
Xue D D, Guo H L, Zheng Y Q, et al. Hybrid identification of progenies of Zoysia crosses by SRAP marker. Acta Prataculturae Sinica, 2009, 18(1): 72-79.
Deng Y, Jiang J, Chen S, et al. Combination of multiple resistance traits from wild relative species in Chrysanthemum via trigeneric hybridization. PLoS One, 2012, 7(8): 1-15.
Yu Z, Xie R, Yu X X, et al. SSR analysis of sorghum-sudangrass new strains with low hydrocyanic acid content. Acta Prataculturae Sinica, 2014, 23(1): 223-228.
Mukai Y, Nakahara Y, Yamamoto M. Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome, 1993, 36(3): 489-494.
Kruppa K, Sepsi A, Szakacs E, et al. Characterization of a 5HS-7DS.7DL wheat-barley translocation line and physical mapping of the 7D chromosome using SSR markers. Journal of Applied Genetics, 2013, 54(3): 251-258.
Sanchez-Moran E, Benavente E, Orellana J. Simultaneous identification of A, B, D and R genomes by genomic in situ hybridization in wheat-rye derivatives. Heredity, 1999, 83(3): 249-252.
Li C, Zhang D, Ge S, et al. Differentiation and inter-genomic relationships among C, E and D genomes in the Oryza officinalis complex (Poaceae) as revealed by multicolor genomic in situ hybridization. Theoretical and Applied Genetics, 2001, 103(2-3): 197-203.
Maluszynska J, Hasterok R. Identification of individual chromosomes and parental genomes in Brassica juncea using GISH and FISH. Cytogenetic and Genome Research, 2005, 109(1-3): 310-314.
Zhang H J, Wang H G, Liu S B, et al. Advances of wheat polygeneric hybrids. Journal of Shangdong Agricultural University, 2000, 31(3): 337-344.
Li Y P, Jiang B, Li Y X, et al. Molecular cytogenetic detection of offspring in wheat trigeneric hybridization. Molecular Plant Breeding, 2013, 11(2): 163-167.
Zhao J X, Fu J, Wu J, et al. Studies on cytogenetics and morphology of the progenies of trigeneric hybridization involving Triticum, Leymus and Thinopyrum. Acta Agriculturae Boreali-Occidentalis Sinica, 2001, 10(4): 20-24.
Zhu W Y, Liu X C, Fang W M, et al. Genetic presentation of BC1 between ‘Zhongshanjingui’ and ‘Zhongshanjingui’ ×Ajania przewalskii. Scientia Agricultura Sinica, 2012, 45(18): 3812-3819.
Li X L, Chen L. Breeding for triploids of Salvia miltiorrhiza and its sustainable utilization. Chinese Traditional and Herbal Drugs, 2012, 43(2): 375-379.
Liu Q C. Genetics. Beijing: Science Press, 2007.
Gan L, Yu X X, Yu Z, et al. A study on main agronomic trasits of yield and quality of clone lines of Solanum truberosum hybrids F₁. Acta Prataculturae Sinica, 2013, 22(4): 312-318.
唐祈林, 李晚忱, 宋运淳, 等. 玉米与四倍体多年生玉米代换种质的选育及其基因组原位杂交鉴定. 遗传学报, 2004, 31(4): 340-344.
任勇, 陈柔屹, 唐祈林, 等. 新型饲草玉米生长动态及收割期的研究. 作物学报, 2007, 33(8): 1360-1365.
唐祈林, 王培, 卢艳丽, 等. 玉蜀黍属物种间遗传关系的RAPD分析. 草业学报, 2009, 18(4): 154-160.
李冬郁, 郭乐群. 玉米野生近缘种类玉米的研究和利用. 玉米科学, 2001, 9(2): 11-13.
冯云超, 唐祈林, 荣廷昭. 玉草2号、川单14×墨西哥大刍草和墨西哥大刍草产量和饲用价值比较. 玉米科学, 2011, 19(3): 68-72.
苏月贵. 玉米、摩擦禾、四倍体多年生类玉米三元杂种的分子细胞遗传学研究. 雅安: 四川农业大学, 2009.
张丽英. 饲料分析及饲料质量检测技术. 北京: 中国农业大学出版社, 2002: 45-80.
杨秀燕, 蔡毅, 傅杰, 等. 玉米及其近缘种大刍草的核型研究. 中国农业科学, 2011, 44(7): 1307-1314.
唐祈林, 荣廷昭. 玉米近缘野生材料和自交系48-2及其远缘杂种F₁过氧化物酶同工酶分析. 四川农业大学学报, 2003,21(1): 10-17.
张海琴, 周永红, 郑有良, 等. 长芒猬草与华山新麦草属间杂种的形态学和细胞学研究. 植物分类学报, 2002, 40(5): 421-427.
薛丹丹, 郭海林, 郑轶琦, 等. 结缕草属植物杂交后代杂种真实性鉴定——SRAP分子标记. 草业学报, 2009, 18(1): 72-79.
于卓, 谢锐, 于肖夏, 等. 低氢氰酸含量高丹草新品系及其亲本的SSR分析. 草业学报, 2014, 23(1): 223-228.
张红军, 王洪刚, 刘树兵, 等. 小麦族多属杂种的研究进展. 山东农业大学学报: 自然科学版, 2000, 31(3): 337-344.
厉永鹏, 姜博, 李宇新, 等. 小麦三属杂交后代的分子细胞遗传学检测. 分子植物育种, 2013, 11(2): 163-167.
赵继新, 傅杰, 武军, 等. 小麦-滨麦-偃麦草三属杂交后代的细胞遗传学和形态学研究. 西北农业学报, 2001, 10(4): 20-24.
朱文莹, 刘新春, 房伟民, 等. 小菊品种‘钟山金桂’与亚菊属细裂亚菊F₁回交后代的性状遗传表现. 中国农业科学, 2012, 45(18): 3812-3819.
李秀兰, 陈力. 三倍体丹参的培育及其可持续利用研究. 中草药, 2012, 43(2): 375-379.
刘庆昌. 遗传学. 北京: 科学出版社, 2007.
甘霖, 于肖夏, 于卓, 等. 马铃薯杂种F₁无性株系产量品质等主要农艺性状研究. 草业学报, 2013, 22(4): 312-318.

新型饲草玉淇淋58的性状、遗传及饲草潜力

Chromosomal composition and forage production potential of Yuqilin 58

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