干旱胁迫下结缕草叶片抗坏血酸与相关生理指标变化的品种差异研究

doi:10.11686/cyxb20130413

摘要/Abstract

摘要： 通过盆栽试验,以日本结缕草与细叶结缕草为材料,研究了干旱胁迫下结缕草叶片AsA含量与相关生理指标的品种差异。结果表明,干旱胁迫下日本结缕草和细叶结缕草叶片中AsA含量与相关生理指标的动态变化规律基本相似,但品种间差异显著。干旱胁迫下2个品种的AsA含量和AsA/DHA均呈现先上升后下降的变化趋势,但日本结缕草下降的时间更早。干旱胁迫下叶片相对含水量、POD活性在2个品种中均呈下降趋势,但日本结缕草的下降趋势更明显,尤其在干旱胁迫后期。胁迫后日本结缕草叶绿素a与叶绿素b含量下降明显,而细叶结缕草变化不大。胁迫后两者的相对电导率和MDA含量均呈上升趋势,但日本结缕草增加的幅度更为显著。干旱胁迫后日本结缕草和细叶结缕草叶片Rubisco的含量均在第3天开始下降,胁迫后期日本结缕草Rubisco含量恢复到对照水平,而细叶结缕草Rubisco含量在胁迫后期显著高于对照。本研究结果表明,干旱胁迫下结缕草可以通过增加和维持叶片中AsA含量与POD活性来减缓Rubisco及叶绿素的降解,降低干旱胁迫对植株造成的氧化胁迫伤害程度,进而改善其抗旱能力。本研究结果进一步证实结缕草抗旱能力有品种差异,其中日本结缕草抗旱能力弱于细叶结缕草。

Abstract: The changes of foliar ascorbic acid and related physiological indexes in Zoysia japonica and Z. tenuifolia under drought stress were studied in a pot trial. The results showed that the dynamic change rules of foliar ascorbic acid and related physiological indexes were similar in Z. japonica and Z. tenuifolia under drought stress, but significant differences were still observed in these two cultivars. For example, the level and redox status of ascorbic acid in these two cultivars were increased at the early drought stress stages, and then decreased at the later drought stress stages, but the decreased time was earlier in Z. japonica compared with Z. tenuifolia. Downtrend of leaf relative water content and peroxidase (POD) activity were found under drought stress in these two cultivars, but the downtrend in Z. japonica was more significant, especially at the later drought stress stages. In addition, the decreases of chlorophyll a and b were significant in Z. japonica but not in Z. tenuifolia under drought stress. Moreover, downtrend of the leaf relative electric conductivity and MDA content were also found in these two cultivars under drought stress, but the downtrend in Z. japonica was more significant. On the other hand, the Rubisco contents decreased on the 3rd day under drought stress in these two cultivars, and no significant difference in the level of Rubisco was observed in Z. japonica relative to the control at the later stages of drought stress, while the difference was significant in Z. tenuifolia. All these results showed that the increase of foliar ascorbic acid and POD activity in Zoysia under drought stress may delay the degradation of Rubisco and chlorophyll content and relieve the injury caused by oxidative stress and improve the drought-resistance. These results also confirmed that there are some drought-resistant difference between different Zoysia cultivars and the drought-resistant of Z. japonica is lower than Z. tenuifolia.

中图分类号:

俞乐,刘拥海,周丽萍,梁国球. 干旱胁迫下结缕草叶片抗坏血酸与相关生理指标变化的品种差异研究[J]. 草业学报, 2013, 22(4): 106-115.

YU Le, LIU Yong-hai, ZHOU Li-ping, LIANG Guo-qiu. A study on the changes of ascorbic acid and related physiological indexes
in different cultivars of Zoysia under drought stress[J]. Acta Prataculturae Sinica, 2013, 22(4): 106-115.

参考文献

李亚, 刘建秀, 向其伯. 结缕草属种质资源研究进展. 草业学报, 2002, 11(2): 7-14.
郭海林, 陈宣, 薛丹丹, 等. 结缕草属植物部分外部性状的遗传分析. 草业学报, 2012, 21(5): 134-143.
胡化广, 刘建秀, 周志芳, 等. 结缕草属植物抗旱生理机理. 草地学报, 2008, 16(2): 141-144.
宋家壮, 李萍萍, 付为国. 水分胁迫及复水对虉草生理生化特性的影响. 草业学报, 2012, 21(2): 62-69.
李松茂, 李森, 李育慧. 中国近50年旱灾灾情分析. 中国农业气象, 2003, 24(1): 7-10.
Asada K. Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiology, 2006, 141: 2391-2396.
Kerchev P I, Pellny T K, Vivancos P D, et al. The transcription factor ABI4 is required for the ascorbic acid-dependent regulation of growth and regulation of jasmonate-dependent defense signaling pathways in Arabidopsis. Plant Cell, 2011, 23: 3319-3334.
White R H, Engelke M C, Morton S J. Irrigation water requirement of zoysiagrass. International Turfgrass Society Research Journal, 1993, 7: 587-593.
李锦树, 王洪春, 王文英, 等. 干旱对玉米叶片细胞透性及膜脂的影响. 植物生理学报, 1983, 9(3): 223-229.
赵世杰, 许长城, 邹琦, 等. 植物组织中丙二醛测定方法的改进. 植物生理学通讯, 1991, 30(3): 207-210.
Kampfenkel K, Motagu M V, Inzè D. Extraction and determination of ascorbate and dehydroascorbate from plant tissue. Analytical Biochemistry, 1995, 225: 165-167.
Amako K, Chen G X, Asada K. Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants. Plant and Cell Physiology, 1994, 35(3): 497-504.
舒展, 张晓素, 陈娟, 等. 叶绿素含量测定的简化. 植物生理学通讯, 2010,46(4): 399-402.
刘拥海, 彭新湘, 李明启. 水稻叶片中过氧化氢与核酮糖-1,5-二磷酸羧化酶/加氧酶降解的关系. 植物生理学报, 2000, 26(6): 481-486.
华珞, 郑海. 草坪栽培养护研究及其问题分析. 中国农业科学, 2004, 37(3): 422-430.
Shao H B, Chen X Y, Chu L Y. Investigation on the relationship of proline with wheat anti-drought under soil water deficits. Biointerfaces, 2006, 53(2): 113-119.
单长卷, 韩蕊莲, 梁宗锁. 黄土高原冰草叶片抗坏血酸和谷胱甘肽合成及循环代谢对干旱胁迫的生理响应. 植物生态学报, 2011, 35: 653-662.
杜润峰, 郝文芳, 王龙飞. 达乌里胡枝子抗氧化保护系统及膜脂过氧化对干旱胁迫及复水的动态响应. 草业学报, 2012, 21(2): 51-61.
周兴元, 曹福亮, 陈国华. 两种暖季型草坪禾草对土壤持续干旱胁迫的生理反应. 草业学报, 2004,13(1): 84-88.
卢少云, 陈斯平, 陈斯曼, 等. 三种暖季型草坪草在干旱条件下脯氨酸含量和抗氧化酶活性的变化. 园艺学报, 2003, 30(3): 303-306.
张文婷, 刘富强, 王华田, 等. 城市绿地植物萱草和结缕草的抗旱性研究. 中国农学通报, 2008, 24(8): 327-331.
Fu J, Huang B. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grass to localized drought stress. Environmental and Experimental Botany, 2001, 45(2): 105-114.
Sharma P, Dubey R S. Drought induced oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regulation, 2005, 46: 209-221.
Selote D S, Khanna-Chopra R. Drought acclimation confers oxidative stress tolerance by inducing co-ordinated antioxidant defense at cellular and subcellular level in leaves of wheat seedlings. Physiologia Plantarum, 2006, 127: 494-506.
王齐, 孙吉雄, 安渊. 水分胁迫对结缕草种群特征和生理特性的影响. 草业学报, 2009, 18(2): 33-38.
曹慧, 周磊. 水分胁迫下非气孔因素对葡萄叶片光合作用的影响. 潍坊学院学报, 2007, 7(2): 56-61.
孔照胜, 武云帅, 岳爱琴, 等. 不同大豆品种抗旱性生理指标综合分析. 华北农学报, 2001, 16(3): 40-45.
陈由强, 朱锦懋, 叶冰莹. 水分胁迫对芒果(Mangifera indica L.) 幼苗细胞活性氧伤害的影响. 生命科学研究, 2000, 4(1): 60-64.
Jeon M W, Ali M B, Hahn E J, et al. Photosynthetic pigments, morphology and leaf gas exchange during ex vitro acclimatization of micropoagated CAM Doritaenopsis plantlets under relative humidity and air temperature. Environmental and Experimental Botany, 2006, 55: 183-194.
曹昀, 王国祥. 土壤水分含量对菖蒲(Acorus calamus)萌发及幼苗生长发育的影响. 生态学报, 2007, 27(5): 1749-1755.
鲍思伟, 谈锋, 廖志华. 土壤干旱对蚕豆叶片渗透调节能力的影响.西南师范大学学报(自然科学版), 2001, 26(4): 448-451.
程智慧, 孟焕文, Rolfe S A, 等. 水分胁迫对番茄叶片气孔传导及光合色素的影响.西北农林科技大学学报(自然科学版), 2002, 30(6): 93-99.
梅杨, 李海蓝, 谢晋, 等. 核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco).植物生理学通讯, 2007, 43(2): 363-368.
Carmo-Silva A E, Keys A J, Andralojc P J, et al. Rubisco activities: properties and regulation in three different C₄ grasses under drought. Journal of Experimental Botany, 2010, 61: 2355-2366.

[1]	郭郁频，米福贵，闫利军，任永霞，吕世杰，伏兵哲. 不同早熟禾品种对干旱胁迫的生理响应及抗旱性评价[J]. 草业学报, 2014, 23(4): 220-228.
[2]	杨海霞，刘润进，郭绍霞. AM真菌摩西球囊霉对盐胁迫条件下高羊茅生长特性的影响[J]. 草业学报, 2014, 23(4): 195-203.
[3]	韩朝,刘洋,董慧,常智慧. 污泥对高羊茅抗旱性的影响研究[J]. 草业学报, 2014, 23(3): 127-135.
[4]	张怀山,赵桂琴,栗孟飞,夏曾润,王春梅. 中型狼尾草幼苗对PEG、低温和盐胁迫的生理应答[J]. 草业学报, 2014, 23(2): 180-188.
[5]	彭燕,李州. 干旱预处理对抗旱性不同的2个草地早熟禾品种耐热性能的影响[J]. 草业学报, 2013, 22(5): 229-238.
[6]	陈敏,侯新村,范希峰,武菊英,潘远智. 细叶芒苗期耐旱性分析[J]. 草业学报, 2013, 22(3): 184-.
[7]	蒋乔峰,陈静波,宗俊勤,李珊,褚晓晴,郭海林,刘建秀. 盐胁迫下磷素对沟叶结缕草生长及Na⁺和K⁺含量的影响[J]. 草业学报, 2013, 22(3): 162-.
[8]	李晓宇,蔺吉祥,李秀军,穆春生. 羊草苗期对盐碱胁迫的生长适应及Na⁺、K⁺代谢响应[J]. 草业学报, 2013, 22(1): 201-209.
[9]	陈静波,刘建秀. 狗牙根抗盐性评价及抗盐机理研究进展[J]. 草业学报, 2012, 21(5): 302-310.
[10]	李珊,陈静波,郭海林,宗俊勤,张芳,禇晓晴,蒋乔峰,丁万文,刘建秀. 结缕草属草坪草种质资源的耐盐性评价[J]. 草业学报, 2012, 21(4): 43-51.
[11]	袁学军,王志勇,郑轶琦,刘建秀,佘建明. 假俭草体细胞抗寒突变体的获得及其SRAP分子鉴定[J]. 草业学报, 2011, 20(6): 237-244.
[12]	王丹,宣继萍,郭海林,刘建秀. 结缕草的抗寒性与体内碳水化合物、脯氨酸、可溶性蛋白季节动态变化的关系[J]. 草业学报, 2011, 20(4): 98-107.
[13]	董秋丽,夏方山,董宽虎. NaCl胁迫对芨芨草苗期脯氨酸代谢的影响[J]. 草业学报, 2010, 19(5): 71-76.
[14]	王艳,李建龙,姜涛,邓蕾. SA、H₂O₂和6-BA预处理对沟叶结缕草耐寒性的影响[J]. 草业学报, 2010, 19(2): 76-81.
[15]	王艳,李建龙^*,余醉,薛峰. 信号分子H₂O₂调节抗氧化系统提高高羊茅耐热性研究[J]. 草业学报, 2010, 19(1): 89-94.