添加绿汁发酵液、乳酸菌制剂和葡萄糖对象草青贮发酵品质的影响

doi:10.11686/cyxb20130314

草业学报 ›› 2013, Vol. 22 ›› Issue (3): 108-.DOI: 10.11686/cyxb20130314

添加绿汁发酵液、乳酸菌制剂和葡萄糖对象草青贮发酵品质的影响

荣辉^1,2,余成群³,陈杰¹,下条雅敬⁴,邵涛^1*

1.南京农业大学动物科学技术学院饲草调制加工与贮藏研究所,江苏南京 210095;
2.贵州省草业研究所,贵州贵阳550006;
3.中国科学院
地理科学与资源研究所,北京100101;
4.九州大学生物资源与环境学部动物饲料生产与利用研究室,日本福冈 812-8581

出版日期:2013-06-20 发布日期:2013-06-20
通讯作者: E-mail:taoshaolan@yahoo.com.cn
作者简介:huir1891@yahoo.com.cn
基金资助:
国家自然科学基金面上项目(30771530)和江苏省普通高校研究生科研创新计划项目资助。

Effects of FJLB, LAB and glucose addition on fermentation quality
of napier grass (Pennisetum purpureum) silage

Effects of FJLB, LAB and glucose addition on fermentation quality
of napier grass (Pennisetum purpureum) silage
RONG Hui^1,2, YU Cheng-qun³, CHEN Jie¹, SHIMOJO Masataka⁴, SHAO Tao¹
(1.Institute of Ensiling and Processing of Grass, College of Animal Sciences and Technology, Nanjing
Agricultural University, Nanjing 210095, China; 2.Guizhou Institute of Pratacuture, Guiyang
550006, China; 3.Institute of Geographic Sciences and Natural Resources Research, Chinese
Academy of Sciences, Beijing 100101, China; 4.Laboratory of Animal Feed Science,
Division of Animal Science, Department of Animal and Marine Bioresource
Sciences, Faculty of Agriculture, Kyushu University,
Fukuoka 812-8581, Japan)

Online:2013-06-20 Published:2013-06-20

摘要/Abstract

摘要： 为评价添加绿汁发酵液、乳酸菌制剂和葡萄糖对象草青贮发酵品质的影响,试验设6个处理组:即对照组(无添加剂),绿汁发酵液组(FJLB),乳酸菌制剂组(LAB),葡萄糖组(G),绿汁发酵液＋葡萄糖组(FJLB+G),乳酸菌制剂＋葡萄糖组(LAB+G);在青贮第3,7,14,30天开窖,取样分析发酵品质。结果表明,与对照组相比,LAB处理对发酵品质影响不大,FJLB处理显著(P<0.05)降低了乳酸和水溶性碳水化合物含量,显著(P<0.05)提高了pH值、乙酸、丁酸和氨态氮含量,使发酵品质变差,而G、LAB+G和FJLB+G处理均显著(P<0.05)提高了青贮早期的乳酸含量,在整个青贮过程中保持较高的乳酸/乙酸值,并显著(P<0.05)降低了最终青贮饲料的pH和氨态氮含量,但与G处理相比,LAB+G和FJLB+G处理没有显示出更优的效果。综上所述,添加葡萄糖可促进同型乳酸发酵,对象草青贮发酵品质的改善效果优于接种乳酸菌。

Abstract: The effects of adding pre-fermented juice of epiphytic lactic acid bacteria (FJLB), lactic acid bacteria (LAB) and glucose (G) on fermentation quality of napier grass (Pennisetum purpureum) were evaluated. The treatments were control (without additives), FJLB, LAB, G, FJLB+G, and LAB+G. The silos were opened on 3, 7, 14, and 30 days after ensiling and the fermentation quality was analysed. Compared with the control, LAB addition had no significant effect on fermentation quality, while FJLB addition reduced fermentation quality In the FJLB addition treatment, lactic acid and water soluble carbohydrate decreased significantly (P<0.05) while pH, ammonia nitrogen, acetic acid and butyric acid increased significantly (P<0.05). In the G, LAB+G, and FJLB+G addition treatments, lactic acid content increased significantly (P<0.05) at an early stage of ensiling, maintaining a high lactic acid/acetic acid value throughout the fermentation process. pH and ammonia nitrogen content of the final silages decreased significantly (P<0.05), but with no significant difference between the three treatments. In conclusion, glucose addition was more important and efficient in stimulating homofermenter activity and in improving the fermentation quality of napier grass silage than inoculation with lactic acid bacteria (LAB or FJLB).

中图分类号:

S816.6 文献标识码:A 文章编号:1004-5759(2013)03-0108-08
〖

荣辉,余成群,陈杰,下条雅敬,邵涛. 添加绿汁发酵液、乳酸菌制剂和葡萄糖对象草青贮发酵品质的影响[J]. 草业学报, 2013, 22(3): 108-.

参考文献

Shao T, Ohba N, Shimojo M, et al. Effect of adding glucose, sorbic acid and pre-fermented juice on the fermentation quality of guineagrass (Panicum maximum Jacq.) silage. Asian-Australasian Journal of Animal Sciences, 2004, 17(6): 808-813.
Shao T, Wang T, Shimojo M, et al. Effect of ensiling density on fermentation quality of guineagrass (Panicum maximum Jacq.) silage during the early stage of ensiling. Asian-Australasian Journal of Animal Sciences, 2005, 18(9): 1273-1278.
Ohyama Y, Morichi T, Masaki S. The effect of inoculation with lactobacillus plantarum and the addition of glucose at ensiling on the quality of aerated silages. Journal of the Science of Food and Agriculture, 1975, 26(7): 1001-1008.
Shao T, Zhang L, Shimojo M, et al. Fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) silages treated with encapsulated-glucose, glucose, sorbic acid and pre-fermented juices. Asian-Australasian Journal of Animal Sciences, 2007, 20(11): 1699-1704.
Cai Y, Ohmono S, Kumai S. Distribution of lactate fermentation characteristics of lactic acid bacteria on forage crops and grasses. Grassland Science, 1994, 39(4): 420-428.
McDonald P, Hunderon A R, Heron S J E. The Biochemistry of Silage (2th edition). UK: Chalcombe Publications, Marlow, 1991: 81-151.
Hassanat F, Mustafa A F, Seguin P. Effects of inoculation on ensiling characteristics, chemical composition and aerobic stability of regular and brown midrib millet silages. Animal Feed Science and Technology, 2007, 139(1-2): 125-140.
玉柱, 魏馨, 于艳冬, 等. 添加剂对尖叶胡枝子青贮发酵品质及体外消化率的影响. 草业学报, 2009, 18(5): 73-79.
王昆昆, 玉柱, 邵涛, 等. 乳酸菌制剂对不同比例苜蓿和披碱草混贮发酵品质的影响. 草业学报, 2010, 19(4): 94-100.
Ohshima M, Cao L, Kimura E, et al. Influence of addition of fermented green juice to alfalfa ensiled at different moisture contents. Grassland Science, 1997, 43(1): 56-68.
荣辉, 徐安凯, 下条雅敬, 等. 初次刈割象草青贮发酵品质动态. 草地学报, 2009, 17(4): 537-539.
Wang J, Wang J Q, Zhou H, et al. Effects of addition of previously fermented juice prepared from alfalfa on fermentation quality and protein degradation of alfalfa silage. Animal Feed Science and Technology, 2009, 151(3-4): 280-290.
Manyawu G J, Sibanda S, Mutisi C, et al. The effect of pre-wilting and incorporation of maize meal on the fermentation of bana grass silage. Asian-Australasian Journal of Animal Sciences, 2003, 16(6): 843-851.
Shao T, Ohba N, Shimojo M, et al. Dynamics of early fermentation of Italian ryegrass (Lolium multiflorum Lam.) silage. Asian-Australasian Journal of Animal Sciences, 2002, 15(11): 1606-1610.
张刚. 乳酸细菌-基础、技术和应用. 北京: 化学工业出版社, 2006: 76-101.
Yahaya M S, Kimura A, Harai J, et al. Evaluation of structural carbohydrates losses and digestibility in alfalfa and orchardgrass during ensiling. Asian-Australasian Journal of Animal Sciences, 2001, 14(12): 1701-1704.
Uchida S, Kitamura Y. Silage making from tropical pasture grown in south western islands of Japan: I. Effects of various treatments at the ensiling time on quality of Rhodes grass and Napier grass silages. Grassland Science, 1987, 32(4): 369-374.
刘秦华, 张建国, 卢小良. 乳酸菌添加剂对王草青贮发酵品质及有氧稳定性的影响. 草业学报, 2009, 18(4): 131-137.
Cai Y. Development of lactic acid bacteria inoculant for whole crop rice silage in Japan. In: International Symposium on Production and Utilization of Whole Crop Rice for Feed. Busan, Korea, 2006: 85-89.
Bureenok S, Namihira T, Tamaki M, et al. Fermentative quality of guineagrass silage by using fermented juice of the epiphytic lactic acid bacteria (FJLB) as a silage additive. Asian-Australasian Journal of Animal Sciences, 2005, 18(6): 807-811.
Zhang J G, Kawamoto H, Cai Y. Relationships between the addition rates of cellulose or glucose and silage fermentation at different temperatures. Animal Science Journal, 2010, 81(3): 325-330.

添加绿汁发酵液、乳酸菌制剂和葡萄糖对象草青贮发酵品质的影响

Effects of FJLB, LAB and glucose addition on fermentation quality
of napier grass (Pennisetum purpureum) silage

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