草坪草病原菌的抗药性现状及研究进展

摘要/Abstract

摘要： 杀菌剂仍然是防治草坪病害、保证草坪健康、美观的最主要手段,但现代杀菌剂很容易出现抗药性、导致防治草坪病害失败。国外从20世纪60年代就已经开展了草坪病原菌抗药性的相关研究,而我国仍然处于空白状态。笔者从草坪草病原菌抗药性现状、传统抗药性研究方向、现代抗药性分子研究方向及抗药性治理这4个方面对当前草坪草病原菌抗药性研究进行综述。

Abstract: Applying fungicide is still a most important measure for managing turfgrass diseases and maintaining turfgrass health and beauty. However, the resistance development in pathogens had easily led to failures of modern fungicides for turfgrass diseases control. Research on fungicide resistance had been developed in the field of turfgrass abroad from 1960s,but none were found in China. This paper reviewed the research advances in the fungicide resistance of pathogenic fungi on turfgrass, including the status of fungicides resistance on turfgrass, the traditional research fields, the modern molecular research fields and the management strategies of fungicide resistance.

中图分类号:

S431.14

胡健,蒋勤军,韩烈保,王丽. 草坪草病原菌的抗药性现状及研究进展[J]. 草业学报, 2009, 18(2): 194-204.

HU-Jian, JIANG Qin-jun, HAN Lie-bao, WANG Li. Status and research advances on fungicide resistance in turfgrass pathogens[J]. Acta Prataculturae Sinica, 2009, 18(2): 194-204.

参考文献

[1］ Detweiler A R, Vargas J M, Brendt W L. Resistance of Colletotrichum graminicola to benomyl[J］. Proceedings of the 6th International Turfgrass Research Conference,1989,6:359-362.
[2］ Warren C G, Sanders P, Cole H.Sclerotinia homoeocarpa tolerance to benzimidazole configuration fungicides[J］. Phytopathology,1974,64:1139-1142.
[3］ Sanders P L. Failure of metalaxyl to control Pythium blight on turfgrass in Pennsylvania[J］. Plant Disease,1984,68:776-777.
[4］夏汉平.美国草坪业的发展历史、现状及思考[J］.草业科学,2002,19:55-61.
[5］ Couch H B. Diseases of Turfgrasses (3rd edition)[M］. Malabar, Florida: Krieger Publishing Company, 1995.
[6］ Couch H B. Common names of plant diseases. Turfgrass (several cultivated spp.)[J］.Plant Disease, 1985, 69:672-675.
[7］ Jo Y K, Niver A L, Rimelspach J W, et al. Fungicide sensitivity of Sclerotinia homoeocarpa from golf courses in Ohio[J］. Plant Disease, 2006, 90:807-813.
[8］ Vargas J M, Jr. Management of Turfgrass Diseases[M］. Boca Raton, Florida: Lewis Publishers, 1994.
[9］ Cole H, Taylor B, Duich J. Evidence of differing tolerances to fungicides among isolates of Sclerotinia homoeocarpa[J］. Phytopathology,1968,58:683-686.
[10］ Burpee L L. Control of dollar spot of creeping bentgrass caused by an isolate of Sclerotinia homoeocarpa resistant to benzim-idazole and demethylation-inhibitor fungicides[J］.Plant Disease, 1997, 81:1259-1263.
[11］ Golembiewski R C, Vargas J M Jr, Jones A L, et al. Detection of demethylation inhibitor (DMI) resistance in Sclerotinia homoeocarpa populations[J］. Plant Disease, 1995,79:491-493.
[12］ Doney J C, Vincelli P. Cross resistance in Sclerotinia homoeocarpa to DMI fungicides[J］. Phytopathology, 1993, 83:1338.
[13］ Vargas J M, Golembiewski R C, Detweiler A R. Dollar spot resistance to DMI fungicides[J］. Golf Course Management, 1992, 60(3):50-54.
[14］ Hsiang T,Yang L, Barton W. Relative virulence of isolates of Sclerotinia homoeocarpa with varying sensitivity to propiconazole[J］. European Journal of Plant Pathology,1998,104:163-169
[15］张海珊,檀根甲,陈莉. 2种麦冬炭疽病菌生物学特性的研究[J］.草业学报,2008,17(4):118-123.
[16］刘贵华.草坪常见病虫害及防治[J］.西南园艺, 2002, 3:32-33.
[17］ Avila-Adame C, Olaya G, Koller W. Characterization of Colletotrichum graminicola isolates resistant to strobilurin-related QoI fungicides[J］. Plant Disease, 2003, 87:1426-1432.
[18］ Crouch J, Glasheen B M, Clarke B B, et al. Biology, phylogenetic relationships, and fungicide resistance in Colletotrichum graminicola isolates from turfgrass in North America[J］. Phytopathology(Abstract), 2003, 93(Supplement): 18.
[19］ Burpee L L, Stephens S L, Martinez-Espinoza A D. Control of anthracnose caused by a benzimidazole- and QoI-resistant isolate of Colletotrichum graminicola[J］. Fungicide and Nematicide Tests, 2004, 59:16.
[20］ Wong F P. Baseline sensitivity distribution of Colletotrichum graminicola (turfgrass anthracnose) to thiophanate-methyl and detection of resistance in California populations[J］. Phytopathology, 2003, 93(Supplement): 91.
[21］ Wong F P, Midland S L. Sensitivity distributions of california populations of Colletotrichum cereale to the DMI fungicides propiconazole, myclobutanil, tebuconazole, and triadimefon[J］. Plant Disease,2007,91:1536-1546.
[22］ Wong F P, Wilcox W F. Occurrence and distribution of QoI-resistant isolates of Colletotrichum cereale from annual bluegrass in California[J］. Plant Disease, 2007,91:1547-1555.
[23］ Landschool P J, Hoyland B F. Gray leaf spot of perennial ryegrass turf in Pennsylvania[J］. Plant Disease,1992,76:1280-1282.
[24］石仁才,商鸿生,张敬泽. 高尔夫果岭上草坪灰斑病病原菌的分离鉴定[J］. 草业学报,2008,17(1):52-57.
[25］薛福祥. 应引起高度重视的草坪草病害——灰斑病[J］.草原与草坪, 2003,1:11-14.
[26］ Vincelli P. Gray leaf spot, an emerging disease of perennial ryegrass[J］. Turfgrass Trends, 1999, 7: 1-8.
[27］ Vincelli P. Fungicidal control of gray leaf spot[J］. Golf Course Management, 1968, 6: 68-74.
[28］ Vincelli P, Dixon E. Resistance to QoI (strobilurin-like) fungicides in isolates of Pyricularia grisea from perennial ryegrass[J］. Plant Disease, 2002,86:235-240.
[29］ Vincelli P, Dixon E. Resistance to QoI (=strobilurin) fungicides in Pyricularia grisea from perennial ryegrass[J］. Phytopathology, 2001, 91(Supplement): 92.
[30］ Abad Z G, Shew H D, Lucas L T.Characterization and pathogenicity of Pythium species isolated from turfgrass with symptoms of root and crown rot in North Carolina[J］. Phytopathology, 1994, 84: 913-921.
[31］ Sanders P L, Coffey M D, Greer G D, et al. Laboratory induced resistance to fosetyl aluminium in a metalaxyl resistant field isolate of Pythium apahanidermaTum[J］. Plant Disease, 1990, 74: 690-692.
[32］ Gisi U, Sierotzki H, Cook A, et al. Mechanisms influencing the evolution of resistance to Qo inhibitor fungicides[J］.Pest Management Science, 2002, 58: 859-867.
[33］ Moorman G W, Arora N, Kim S H. Propamocarb resistance in species of Pythium[J］. Phytopathology, 2002, 92(Supplement): 58.
[34］ Brent K J, Hollomon D W. Fungicide Resistance in Crop Pathogens: How Can It be Managed? Second Revised Edition[C］. Fungicide Resistance Action Committee, 2007.
[35］刘英华,王开运,姜兴印,等. 植物病原菌抗性生物学及生化机制研究进展[J］.世界农药,2004,26(3):16-18.
[36］许学明,王开运,段海明,等.烟草黑胫病菌抗甲霜灵突变体的诱导及其适合度研究[J］.农药学学报,2008,10(1):35-40.
[37］ Russell P E. Sensitivity Baselines in Fungicide Resistance Research and Management[C］. Fungicide Resistance Action Committee Monograph No.3, 2007.
[38］祁之秋,王建新,陈长军,等.现代杀菌剂抗性研究进展[J］.农药,2006,45(10):655-659.
[39］ Kim Y S, Dixon E W, Vincelli P, et al. Field resistance to strobilurin (QoI) fungicides in Pyricularia grisea caused by mutations in the mitochondrial cytochrome b gene[J］. Phytopathology,2003,93:891-900.
[40］ Gisi H, Pavic L, Stanger C, et al. Dynamics of Mycosphaerella graminicola populations in response to selection by different fungic-ides[A］. Modern Fungicides and Anti-fungal Compounds IV[C］. 14th International Reinhardsbrunn Symposium, AgroConcept,2005.
[41］ Leroux P, Fritz R, Debieu Albertini C, et al. Mechanism of resistance to fungicides in field strains of Botrytis cinerea[J］. Pest Managemnet Science,2002,58:876-888.
[42］ Wong F P, de la Cerda K A, Hernandez-Martinez R, et al. Detection and characterization of benzimidazole resistance in California populations of Colletotrichum cereale[J］. Plant Disease,2008,92:239-246.
[43］ Bartlett D W, Clough J M, Godwin J R, et al. The strobilurin fungicides[J］. Pest Management Science, 2002, 58: 649-662.
[44］ Fungicide Resistance Action Committee. FRAC list of plant pathogenic organisms resistant to disease control agents[EB/OL］.http://www.frac.info/frac/publication/anhang/List_of_resistant_plant_pathogens_Dec%202006_web.pdf,2006.
[45］ Ma Z, Michailides T J. Advances in understanding molecular mechanisms of fungicide resistance and molecular detection of resistant genotypes in phytopathogenic fungi[J］. Crop Protection,2005,24:853-863.
[46］ Koller W, Wilcox W F. Evidence for the predisposition of fungicide-resistant isolates of Venturia inaequalis to a preferential selection for resistance to other fungicides[J］. Phytopathology,2001,91:776-781.
[47］ Wong F P, Wilcox W F. Comparative physical modes of action of azoxystrobin, mancozeb, and metalaxyl against Plasmopara viticola[J］. Plant Disease, 2001, 85: 649-656.
[48］ Wong F P, Wilcox W F. Sensitivity to axoxystrobin among isolates of Uncinula necator: Baseline distribution and relationship to myclobutanil sensitivity[J］. Plant Disease,2002,86:394-404.
[49］ Windass J D, Heaney S P, Renwick A, et al. Methods for detecting low frequencies of mutations in mitochondrially encoded genes[P］. International Patent: WO 00/66773, 2000.
[50］ Brent K J, Hollomon D W. Fungicide Resistance: The Assessment of Risk. Second revised edition[C］. Fungicide Resistance Action Committee, 2007.27.
[51］ Sierotzki H, Parisi S, Steinfeld U, et al. Mode of resistance to respiration inhibitors at the cytochrome bc1 enzyme complex of Mycosphaerella fijiensis field isolates[J］. Pest Management Science, 2000,56: 833-841.
[52］ Sierotzki H, Wullschleger J, Gisi U. Point mutation in cytochrome b gene conferring resistance to strobilurin fungicides in Erysiphe graminis f. sp. tritici field isolates[J］. Pesticide Biochemistry and Physiology, 2000,68: 107-112.
[53］ Steinfeld U, Sierotzki H, Parisi S, et al. Sensitivity of mitochondrial respiration to different inhibitors in Venturia inaequalis[J］. Pest Management Science, 2001,57: 787-796.
[54］ Zheng D, Kller W. Characterization of the mitochondrial cytochrome b gene from Venturia inaequalis[J］. Current Genetics, 1997,32: 361-366.
[55］ Wille P, Sierotzki H, Stanger C, et al. Qualitative and quantitative identification of SNPs in plant pathogens[A］. Modern Fungicides and Antifungal Compounds III[C］. Alton UK: British Crop Production Council, 2002.
[56］ Sierotzki H, Gisi U. Molecular diagnostics for fungicide resistance in plant pathogens[A］. Chemistry of Crop Protection[M］. Weinheim: Wiley-VCH, 2003. 71-88.
[57］ Fraaije B A, Butters J A, Coelho J M, et al. Following the dynamics of strobilurin resistance in Blumeria graminis f.sp. tritici using quantitative allele-specific real-time PCR measurements with the fluorescent dye SYBR green[J］. Plant Pathology,2002,51:45-54.
[58］ Olaya G, Cleere S, Stanger C, et al. A novel potential target site QoI fungicide resistance mechanism in Pythium aphanidermatum[J］. Phytopathology(Abstract), 2003,93(Supplement):67.
[59］ Leadbeater A,Sierotzki H,Varney PA, et al. List of pathogens with field resistance towards QoI fungicides. Fungicide resistance action committee QoI working group[EB/OL］. http://www.frac.info/frac/meeting/2007/Pathogens with field resistance towards QoI fungicides.pdf, 2007-11-28.
[60］ Maymon M, Zveibil A, Pivonia S, et al. Identification and characterization of benomyl-resistant and -sensitive populations of Colletotrichum gloeosporioides from statice[J］. Phytopathology,2006,96:542-548.
[61］ Panaccione D G, Hanau R M. Characterization of two divergent beta-tubulin genes from Colletotrichum graminicola[J］. Gene,1990,86:163-170.
[62］陆悦健,周明国,叶钟音,等.抗苯并咪唑的小麦赤霉病菌β-tubulin基因序列分析与特性研究[J］. 植物病理学报, 2000,30(1):30-34.
[63］ Kawchuk L M, Hutchison L J, Verhaeghe C A,et al. Isolation of the β-tubulin gene and characterization of thiabendazole resistance in Gibberella pulicaris[J］. Canadian Journal of Plant Pathology, 2002,24:233-238.
[64］ Miller G L, Stevenson K L, Burpee L L. Sensitivity of Sclerotinia homoeocarpa isolates to propiconazole and impact on control of dollar spot[J］. Plant Disease,2002,86:1240-1246.
[65］ De Waard M A, Andrade A C, Hayashi K, et al. Impact of fungal drug transporters on fungicide sensitivity, multidrug resistance and virulence[J］. Pest Management Science,2006,62:195-207.
[66］ Schnabel G, Jones A L. The 14 а-demethylase (CYP51A1) gene is over expressed in Venturia inaequalis strains resistant to myclobutanil[J］. Phytopathology,2000,91:102-110.
[67］ Guan J, Van Leemputl, Posthumus M, et al. Metabolism of imazalil by wild-type and DMI resistant isolates of Penicillium italicum[J］. Netherland Journal of Plant Pathology, 1992,98:161-168.
[68］ Ian B D, Khor H Y, Hutton D G. Dicarboximide resistance in field isolates of Alternaria alternatais mediated by a mutation in a two-component histidine kinase gene[J］. Fungal Genetic and Biology, 2004,41:102-108.
[69］石志琦, 周明国, 叶钟音. 核盘菌对菌核净的抗药性机制初探[J］. 农药学学报, 2000, 2: 47-51.
[70］祝明亮,罗义勇,李梅云,等. 植物病原真菌对内吸性杀菌剂的抗药性研究进展[J］. 烟草农业科学,2006,2(4):324-329.
[71］刘洪斌, 顾宝根, 刘西莉, 等. 马铃薯晚疫病菌对甲霜灵抗性机制的初步研究[J］. 植物病理学报, 2003, 33(2): 178-182.
[72］ Felsenstein F G. Sensitivity of Erysiphe graminis f. sp. Tritici to demethylation inhibiting fungicides in Europe[R］. British Crop Protection Council, Farnham. Surrey, 1994.35-42.
[73］ Devries R E, Trigiano R N, Windham M T, et al. Genetic analysis of fungicide-resistant Sclerotinia homoeocarpa isolates from Tennessee and northern Mississippi[J］. Plant Disease, 2008,92:83-90.
[74］吴小芹.林木病原真菌的群体分化研究[J］.林业科学研究,2000,13(4):423-430.
[75］ Harman G E, Nelson E B, Ondik K L. Diversity and biology of the dollar spot organism Sclerotinia homoeocarpa and its implications[J］. The US Golf Association Turfgrass and Environment Research, 2005, 4:1-9.
[76］ Tredway L P. Fungicides for turfgrass disease control[EB/OL］. http://www.frac.info,2007.
[77］杨亚君,陶晡,李川,等. 腐霉枯萎病菌毒素在坪草抗病性筛选中的应用研究[J］.草业学报,2008,17(3);93-98.
[78］ Genet J L, Jaworska G, Deparis F. Effect of dose rate and mixtures of fungicides on selection for QoI resistance in populations of Plasmopara viticola[J］. Pest Management Science,2006,62:188-194.
[79］ Lo C T,Nelson E B,Harman G E.Improved biocontrol efficacy of Trichoderma harzianum 1295-22 for foliar phases of turf diseases by use of spray applications[J］.Plant Disease,1997,81:1132-1138.

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