旗草内生真菌与旗草抗病性研究

摘要/Abstract

摘要： 旗草内生真菌交织顶孢霉的7个不同分离物对旗草主要病源真菌德氏霉和立枯丝核菌在PDA培养基上的体外对峙试验表明,大多数内生真菌分离物对这2个病原菌都有不同程度的抑制作用,不同分离物对同一病原菌的抑制作用不同,同一分离物对不同病原菌的抑制作用也不同,EB6780.501和EH32a对德氏霉和立枯丝核菌的抑制作用最强。旗草植株抗病试验表明,内生真菌感染的植株对叶枯病具有明显的抗性,但这种抗性随着病原菌立枯丝核菌入侵时间的延长而减弱。在体外抑菌试验中,对病原菌生长抑制能力强的内生真菌分离物,在旗草体内也表现出较强的抗病性,说明抗病内生真菌的体外抑菌筛选是有效的。

Abstract: Plate confrontation test of 7 isolates of fungal endophytes on Brachiaria spp. against pathogenic fungi Drechslera sp. and Rhizoctonia solani on PDA showed that most of the isolates had inhibition effect to the both pathogenic fungi. Different isolates had different antagonistic effect to the same pathogenic fungi, and the same isolate also had different antagonistic effect to different pathogenic fungi. Among of endophyte isolates, EB6780.501 and EH32a showed the best inhibition effect to both D. sp. and R. solani. In vivo test showed endophyte infected plants clearly resistance to foliar blight caused by R. solani. But the effectiveness became less as time going on after inoculation of R. solani. There was correlation between in vitro and in vivo test, the isolates with strong in vitro inhibitory effects also showed strong plant protection. Since in vitro inhibition test is much easier and simpler to do than in vivo test, it is better to make an inhibition in vitro test before in vivo in the selection of endophyte strains with disease-resistance.

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黄东益,黄小龙,SEGENET Kelemu. 旗草内生真菌与旗草抗病性研究[J]. 草业学报, 2009, 18(2): 39-45.

HUANG dong-yi, HUANG Xiao-long, SEGENET kelemu. Research on fungal endophytes on Brachiaria spp. and their resistance to pathologenic fungi[J]. Acta Prataculturae Sinica, 2009, 18(2): 39-45.

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旗草内生真菌与旗草抗病性研究

Research on fungal endophytes on Brachiaria spp. and their resistance to pathologenic fungi

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[1]	郭玉霞,南志标,王成章,李春杰,沈禹颖,严学兵. 苜蓿根部入侵真菌研究进展[J]. 草业学报, 2009, 18(5): 243-249.
[2]	山宝琴,贺学礼,段小圆. 毛乌素沙地密集型克隆植物根围AM真菌多样性及空间分布[J]. 草业学报, 2009, 18(2): 146-154.