新麦草愈伤组织多倍体诱导与倍性鉴定

草业学报 ›› 2010, Vol. 19 ›› Issue (6): 126-131.

新麦草愈伤组织多倍体诱导与倍性鉴定

云岚,云锦凤,李俊琴,郑丽娜,赵韦,齐丽娜

内蒙古农业大学生态环境学院,内蒙古呼和浩特 010018

收稿日期:2010-04-07 出版日期:2010-06-25 发布日期:2010-12-20
作者简介:云岚(1970-),女,蒙古族,内蒙古呼和浩特人,副教授,博士。 E-mail: yunlan@imau.edu.cn
基金资助:
国家科技支撑计划项目(2008BADB3B03)和内蒙古科技厅重大项目(20071923)资助。

Callus polyploidy induction and identification of Russian wild ryegrass

YUN Lan, YUN Jin-feng, LI Jun-qin, ZHENG Li-na, ZHAO Wei, QI Li-na

College of Ecological and Environmental Science, Inner Mongolia
Agricultural University, Hohhot 010018, China

Received:2010-04-07 Online:2010-06-25 Published:2010-12-20

摘要/Abstract

摘要： 以新麦草幼胚形成的胚性愈伤组织为材料,在悬浮培养基中添加不同浓度秋水仙素和1.5%DMSO诱导愈伤组织染色体加倍,并对处理后的愈伤组织再生幼苗进行根尖染色体倍性鉴定。在25℃下用100mg/L秋水仙素和1.5%DMSO处理72h的愈伤组织再生植株,平均加倍细胞比例最大,为53.58%。比较分蘖期加倍幼苗与二倍体幼苗形态差异,结果除分蘖数无显著差异外,四倍体叶片长、宽均显著高于二倍体,与混倍体叶长差异不显著。四倍体新麦草叶片气孔保卫细胞长度比二倍体增加13.52%,差异显著。同时四倍体叶表皮气孔之间距离也显著大于二倍体,混倍体以上气孔特征介于四倍体与二倍体之间。

Abstract: Chromosome doubling of Russian wild rye was induced with colchicine and dimethyl sulfoxide (DMSO) in suspension culture media, and embryogenic calli were induced from immature embryos. Chromosome ploidy of root tip cells was identified after induction. Treatment with 100 mg/L colchicine and 1.5% DMSO for 72 h on callus was the best for polyploidy induction. The percentage of tetraploid cells was 53.58%. Morphological comparisons showed that the length and width of leaves from tetraploid plant was increased. The length of stomatal guard cells of the tetraploids increased by 13.52% more than the diploids and the distance between stomata increased significantly. The stomatal characteristics of mixedploid plants were intermediate between the two.

中图分类号:

云岚,云锦凤,李俊琴,郑丽娜,赵韦,齐丽娜. 新麦草愈伤组织多倍体诱导与倍性鉴定[J]. 草业学报, 2010, 19(6): 126-131.

YUN Lan, YUN Jin-feng, LI Jun-qin, ZHENG Li-na, ZHAO Wei, QI Li-na. Callus polyploidy induction and identification of Russian wild ryegrass[J]. Acta Prataculturae Sinica, 2010, 19(6): 126-131.

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