腐殖质在Comamonas koreensis CY01介导的2,4-二氯苯氧乙酸还原脱氯过程中的作用

草业学报 ›› 2011, Vol. 20 ›› Issue (1): 248-252.

腐殖质在Comamonas koreensis CY01介导的2,4-二氯苯氧乙酸还原脱氯过程中的作用

王弋博^1,2,武春媛²,周顺桂²

1.天水师范学院生命科学与化学学院,甘肃天水 741000;
2.广东省生态环境与土壤研究所, 广东广州 510650

出版日期:2011-02-22 发布日期:2011-02-22
作者简介:王弋博(1973-),女,甘肃天水人,教授,博士。
基金资助:
国家自然基金项目(31060065),甘肃省自然基金项目(3zs051-A25-026)和天水师范学院‘青蓝’人才工程基金资助。

Effect of humic substances on the reductive dechlorination of 2,4-dichlorophenoxyacetic
acid by Comamonas koreensis CY01

WANG Yi-bo^1,2, WU Chun-yuan², ZHOU Shun-gui²

1.School of Life Science and Chemistry, Tianshui Normal University, Tianshui 741001, China;

2.Guangdong Institute of Eco-environment and Soil Sciences, Guangzhou 510650, China

Online:2011-02-22 Published:2011-02-22

摘要/Abstract

摘要： 从广东四会原始森林土壤中分离到一株能高效还原Fe(III)和腐殖质(HS)的兼性厌氧菌,编号为CY01,该菌株被鉴定为Comamonas koreensis(韩国丛毛单胞菌)。以Comamonas koreensis CY01为研究对象,研究了厌氧条件下HS(腐殖质)的模式物AQDS对微生物介导的2,4-D (2,4-二氯苯氧乙酸)还原脱氯过程的影响。研究结果显示,1)CY01具有使2,4-D进行脱氯降解的能力, CY01引起的2,4-D的脱氯降解过程是伴随着电子供体葡萄糖的氧化同时进行的;2)反应体系中加入AQDS,会促进2,4-D的降解过程,AQDS在Fe(III)/HS还原菌和2,4-D之间充当电子穿梭体。综合以上实验结果表明, CY01菌株引起的2,4-D的还原脱氯过程的完成是随着电子供体的氧化产生电子,然后AQDS将产生的电子加速传递至2,4-D,使2,4-D发生还原脱氯降解。

Abstract: A facultative anaerobic bacterium which was a highly efficient humic substances and Fe (III)-reducing bacterium (designated CY01), was isolated from an ancient forest soil from Guangzhou, China and was identified as a strain of Comamonas koreensis. The role of the humic model compound, anthraquinone-2,6-disulfonate (AQDS), in the anaerobic reductive dechlorination of 2,4-dichlorophenoxyacetic acid (2,4-D) by C. koreensis CY01 was investigated. 1) Strain CY01 could couple glucose oxidation to 2,4-D reductive dechlorination; 2) Reductive dechlorination of 2,4-D by strain CY01 was greatly stimulated by the addition of AQDS which acts as an electron shuttle. 2,4-D reduction proceeded by oxidation of electron donors and transference of electrons to the acceptors through the redox mediator, AQDS.

中图分类号:

Q946

王弋博,武春媛,周顺桂. 腐殖质在Comamonas koreensis CY01介导的2,4-二氯苯氧乙酸还原脱氯过程中的作用[J]. 草业学报, 2011, 20(1): 248-252.

WANG Yi-bo, WU Chun-yuan, ZHOU Shun-gui. Effect of humic substances on the reductive dechlorination of 2,4-dichlorophenoxyacetic
acid by Comamonas koreensis CY01[J]. Acta Prataculturae Sinica, 2011, 20(1): 248-252.

参考文献

Busa J S, Hammond L E. Regulatory progress, toxicology, and public concerns with 2, 4-D: Where do we stand after two decades. Crop Protection, 2007, 26: 266-269.
李小坤, 鲁剑巍, 陈防. 牧草施肥研究进展. 草业学报, 2008, 17(2): 136-142.
Neilson J W, Josephson K L, Pillai S D, et al. Polymerase chain reaction and gene probe detection of the 2, 4-dichlorophenoxyacetic acid degradation plasmid, pJP4. Applied & Environmental Microbiology, 1992, 58: 1271-1275.
周萍, 刘国彬, 薛萐. 草地生态系统土壤呼吸及其影响因素研究进展. 草业学报, 2009, 18(2): 184-193.
Curtis G P, Reinhard M. Reductive dehalogenation of hexachloroethane, carbon tetrachloride, and bromoform by anthrahydroquinone disulfate and humic acid. Environmental Science & Technology, 1994, 28: 2393-2401.
Lovley D R, Coates J D, Blunt-Harris E L, et al. Humic substances as electron acceptors for microbial respiration. Nature, 1996, 382: 445-448.
Cervantes F J, Lettinga L, Vu-Thi-Thu G, et al. Quinone-respiratio improves dechlorination of carbon tetrachloride by anaerobic sludge. Applied Microbiology Biotechnology, 2004, 64: 702-711.
Hong Y G, Guo J, Xu Z C, et al. Humic substances act as electron acceptor znd redox mediator for microbial dissimilatory azoreduction by Shewanella decolorationis S12. Journal of Microbiology and Biotechnology, 2007, 17: 428-437.
Straub K L, Schink B. Evaluation of electron-shuttling compounds in microbial ferric iron reduction. FEMS Microbiology Letters, 2003, 220: 229-233.
Apper L, Mcknight D, Robinfulton J, et al. Fulvic acid oxidation state detectionusing fluorescence spectroscopy. Environmental Science & Technology, 2002, 36: 3170-3175.
Miller G L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 1959, 31: 426-428.
王弋博, 武春媛, 周顺桂. 韩国丛毛单胞菌CY01的铁还原和腐殖质还原. 兰州大学学报(自然科学版), 2010, 46(2): 35-39.
Leahy J G, Colwell R R. Microbial degradation of hydrocarbons in the environment. Microbiology Molecular Biology Reviews, 1990, 54: 305-315.
Coates J D, Ellis D J, Blunt-Harris E L, et al. Recovery of humic-reducing bacteria from a diversity of environments. Applied & Environmental Microbiology, 1998, 64: 1504-1509.
Kristina L S, Andreas K, Bernhard S. Enrichment and isolation of ferric-iron- and humic-acid-reducing bacteria. Methods in Enzymology, 2005, 397: 58-70.
Lovley D R, Frage J L, Blunt-Harris E L, et al. Humic substances as a mediator for microbially catalyzed metal reduction. Acta Hydrochimicaet Hydrobiologica, 1998, 26: 152-157.
Loffler F E. Enrichment, cultivation, and detection of reductively dechlorinating bacteria. Methods in Enzymology, 2005, 397: 77-111.
Coates J D. Humic substances as a mediator for microbially catalyzed metal reduction. Acta Hydrochimicaet Hydrobiologica, 1998, 26: 152-157.
Fultz M L, Durst R A. Mediator compounds for the electrochemical study of biological redox systems. Analytica Chimica Acta, 1982, 14: 1-18.
Lovley D R. Bioremediation of organic and metal contaminants with dissimilatory metal reduction. Journal of Industrial MicrobioIogy &Biotechnology, 1995, 14: 85-93.
Lovley D R. Microbial reduction of iron manganese and other metals. Advances in Agronomy, 1995, 54: 175-231.
Lovley D R, Coates I D, Saffarini D A, et al. Dissimilatory iron reduction. In: Winkelman, Carrano C. Iron and Related Transition Metals in Microbial Metabolism. Switzerland: Harwood Academic Publishers, 1997: 187-215.

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