蒺藜苜蓿全基因组中U-box基因家族的筛选与特征分析

doi:10.11686/cyxb2015102

摘要/Abstract

摘要： 植物基因组中广泛存在U-box基因,其编码蛋白大部分作为泛素系统中决定底物特异性识别的E₃泛素连接酶,广泛地调控植物生长生殖发育以及响应逆境胁迫等过程。本文利用蒺藜苜蓿基因组数据库,通过生物信息学手段,鉴定蒺藜苜蓿U-box家族基因;采用MEGA6软件进行系统进化树分析;通过GSDS在线工具和Mapinspect软件进行基因结构及染色体定位分析;利用已有的蒺藜苜蓿芯片数据进行组织表达和胁迫响应表达分析。结果表明,蒺藜苜蓿基因组中含有41个U-box基因,不均匀地分布于蒺藜苜蓿的8条染色体上。根据结构域组成和系统进化分析将这些U-box蛋白分成6类。基因表达模式分析发现,该家族成员的表达具有一定的组织特异性,并能响应盐、干旱和氮素胁迫。这些研究结果为蒺藜苜蓿U-box基因家族的功能分析奠定了基础。

Abstract: The U-box domain genes typically exist in multiple variants within plant genomes, and most of them belong to the ubiquitin/proteasome system as E₃ ubiquitin ligase. They play key roles in the regulation of growth, in development, in reproduction as well as in response to environmental stress. In this study, bioinformatics methods were applied to identify U-box family genes of M. truncatula through whole genome screening. A phylogenetic tree was created using the MEGA6 program. Gene structure and chromosome location were defined by GSDS and Mapinspect. A total of 41 U-box genes distributed unevenly on 8 chromosomes were identified from M. truncatula. The U-box proteins were classified into six major classes based on their motif compositions and phylogeny relationships. The expression profiles of M. truncatula U-box genes showed characteristics of tissue specificity and response to salt, drought and nitrogen stress. These results will be useful for future functional analyses of the U-box family genes in M. truncatula.

郑兴卫, 邵麟惠, 李聪. 蒺藜苜蓿全基因组中U-box基因家族的筛选与特征分析[J]. 草业学报, 2015, 24(8): 130-141.

ZHENG Xing-Wei, SHAO Lin-Hui, LI Cong. Genome-wide screening and characterization of the U-box gene family in Medicago truncatula[J]. Acta Prataculturae Sinica, 2015, 24(8): 130-141.

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