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草业学报 ›› 2015, Vol. 24 ›› Issue (8): 150-158.DOI: 10.11686/cyxb2014321

• 论文 • 上一篇    下一篇

二斑叶螨抗甲氰菊酯种群解毒酶基因表达分析

杨顺义1**, *, 岳秀利1**, *, 王进军2, 刘长仲1, 沈慧敏1*, *, 沈一凡1, 郭金梅1   

  1. 1.甘肃农业大学草业学院,草业生态系统省部共建教育重点实验室,中-美草地畜牧业可持续发展中心,甘肃 兰州 730070;
    2.西南大学植物保护学院,昆虫学及害虫控制工程重点实验室,重庆400716
  • 出版日期:2015-08-20 发布日期:2015-08-20
  • 通讯作者: E-mail: ndshm@gsau.edu.cn
  • 基金资助:
    公益性行业(农业)科研专项 (201103020)和国家自然科学基金项目(31260442)资助; 作者简介:杨顺义(1972-),男,甘肃礼县人,副教授,硕士; E-mail:yangshy@gsau; edu; cn; 岳秀利(1988-),男,山东滕州人,硕士; E-mail:yuexiuli.01@163; com

Gene expression of a detoxification enzyme in Tetranychus urticae resistant to fenpropathrin

YANG Shun-Yi1, **, YUE Xiu-Li1, **, WANG Jin-Jun2, LIU Chang-Zhong1, SHEN Hui-Min1, *, SHEN Yi-Fan1, GUO Jin-Mei1   

  1. 1.College of Prataculture, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem Education Ministry, The Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou 730070, China;
    2.College of Plant Protection, Southwest University, China Key Laboratory of Entomology and Pest Control Engineering, Chongqing 400716, China
  • Online:2015-08-20 Published:2015-08-20

摘要: 基因mRNA水平相对表达量的显著变化是二斑叶螨对拟除虫菊酯类药剂产生抗性的重要机制。为了揭示二斑叶螨对甲氰菊酯抗性产生的解毒酶分子机理,本研究采用实时荧光定量PCR(quantitative real time PCR, qRT-PCR)方法分析二斑叶螨实验室敏感(SS)和田间种群(LZ-R、GN-R、WW-R、TS-R和LX-R)主要解毒酶谷胱甘肽转移酶(glutathione s-transferases, GSTs)、细胞色素P450单加氧酶(cytocheome P450 monooxygenases, P450s) 及羧酸酯酶(carboxyl/cholinesterases, CCEs)基因mRNA水平相对表达量的差异。结果表明,二斑叶螨不同种群不同解毒酶基因的相对表达量不同。WW-R和TS-R种群中TuGSTd05以及LX-R种群中TuGSTd01和 TuGSTd06基因表达量均显著上调,为SS种群的1.42~2.34倍,而GN-R种群中TuGSTd04,LZ-R种群中TuGSTd05 和GSTd09表达量显著下调,为SS种群的0.41~0.70倍;P450s基因CYP406A1 和CYP4CL1表达量在LZ-R、GN-R以及WW-R种群中均显著上调,分别为SS种群的1.80~4.88倍,此外,CYP387A1在LZ-R种群中显著上调2.19倍,而在LX-R种群中显著下调0.42倍;CCEs基因TuCCE-35表达量在WW-R和TS-R种群中显著上调,分别为SS种群的2.82和3.09倍,而TuCCE-36基因在所有种群中的表达量均不显著。二斑叶螨不同种群中解毒酶基因GSTs、P450s 和CCEs的显著上调或下调可能与甲氰菊酯的抗性形成有关。

Abstract: The variation of mRNA gene expression is an important pyrethroid insecticide resistance mechanism in Tetranychus urticae. To identify the molecular mechanism for producing detoxification enzymes in T. urticaein response to fenpropathrin, the mRNA expression levels of glutathione s-transferases (GSTs), cytochrome (P450s) and carboxyl/cholinesterases (CCEs) genes in laboratory fenpropathrin susceptible (SS), fenpropathrin resistant (Sp-R) and field strains (LZ-R, GN-R, WW-R, TS-R and LX-R) of T. urticae were measured using quantitative real time PCR (qRT-PCR). The results showed that relative expression levels of different detoxification enzyme genes varied with different strains of T. urticae. Compared with the SS strain, the expression levels of TuGSTd05 in the WW-R and TS-R strains, TuGSTd01 and TuGSTd06 in the LX-R strain were significantly up-regulated,1.42-2.34 times greater than the SS strain, while TuGSTd04 in the GN-Rstrain, TuGSTd05 and TuGSTd09 in the LX-R strain were significantly down-regulated, 0.41-0.70 that of the SS strain. The expression levels of P450s genes CYP406A1 and CYP4CL1 in the LZ-R, GN-R and WW-R strains were significantly up-regulated, 1.80-4.88 times that of the SS strain. The expression level of P450s genes CYP387A1 in the LZ-R strain was also significantly higher (2.19 times) than the SS strain whereas that in the LX-R strain was significantly lower (0.42 times) than the SS strain. Similarly, the expression levels of CCEs gene TuCCE-35 in the WW-R and TS-R strains was significantly higher than the SS strain. However, the gene expression levels of TuCCE-36 did not change in all strains. The significant up-regulation or down-regulation of detoxification enzyme genes (GSTs, p450s and CCEs) among different strains of T. urticae may be associated with the formation of resistance to fenpropathrin.