转玉米ZmABI3-L基因增加拟南芥的抗旱和耐盐性

doi:10.11686/cyxb2015330

草业学报 ›› 2016, Vol. 25 ›› Issue (2): 124-131.DOI: 10.11686/cyxb2015330

转玉米ZmABI3-L基因增加拟南芥的抗旱和耐盐性

袁进成^**, 宋晋辉^**, 马海莲, 瓮巧云, 王凌云, 赵艳, 刘颖慧^*

河北北方学院农林科技学院,河北张家口 075000

收稿日期:2015-06-30 出版日期:2016-02-20 发布日期:2016-02-20
通讯作者: E-mail: leely519@126.com
作者简介:袁进成(1970-),男,河北张家口人,副教授。E-mail: nkxyjc@163.com。宋晋辉(1978-),女,河北安国人,硕士。E-mail: nkxsjh@163.com。**共同第一作者These authors contributed equally to this work.
基金资助:
国家自然科学基金 (31101155), 河北北方学院重大项目(ZD201305),河北省科技厅项目 (13226326)和河北省高等学科拔尖人才选拔与培养计划(BR2-234)资助

A maize abscisic acid insensitive 3 gene confers drought and salt stress tolerance in Arabidopsis

YUAN Jin-Cheng^**, SONG Jin-Hui^**, MA Hai-Lian, WENG Qiao-Yun, WANG Ling-Yun, ZHAO Yan, LIU Ying-Hui^*

College of Agriculture and Forestry, Hebei North University, Zhangjiakou 075000, China

Received:2015-06-30 Online:2016-02-20 Published:2016-02-20

摘要/Abstract

摘要： ABI3 (abscisic acid insensitive 3) 是编码 ABA 信号转导途径中的重要调控因子,广泛地存在于玉米、小麦、水稻等谷类作物中。本研究从玉米中获得一个新的 ABI3-like 基因,命名为ZmABI3-L,该基因全长1735 bp,开放阅读框1212 bp,编码蛋白含404个氨基酸。同源比对表明ZmABI3-L和谷子、高粱的同源蛋白相似性高,分别为64%和58%。基因的表达分析表明该基因是组成型表达,在幼胚、穗子和花丝中表达量较高,同时基因的转录水平可以为盐、ABA、干旱和冷所诱导。将ZmABI3-L基因转化到拟南芥中,对T₃代转 ZmABI3-L基因拟南芥进行抗逆性分析,结果显示ZmABI3-L基因可以增强拟南芥的耐盐和抗旱能力。在150 mmol/L高盐培养基中转基因拟南芥的根和茎长度分别为对照的 8.6 和 1.4 倍,在50 mmol/L甘露醇的渗透培养基中转基因植株的发芽率是74.5%,而对照仅为 33.6%。研究表明 ZmABI3-L是一个对干旱和盐损伤均有响应而显著上调的基因,同时该基因可以增加拟南芥的抗旱和耐盐性。

Abstract: The abscisic acid insensitive 3 gene (ABI3) has been widely studied in cereals such as wheat, maize and rice however, the functions of ABI3 have not been fully described. In this paper, a novel maize ABI3 like gene was cloned and named ZmABI3-L. This gene was predicted to encode a transcription factor with a distinct DNA-binding B3 domain. The full length of the gene was 1735 bp and with an opening read frame of 1212 bp and encoded 404 amino acids. Alignment of the ZmABI3-L proteins with other plants revealed similarities with ABI3 protein from other species. RT-PCR analysis showed ZmABI3-L was up-regulated in maize by dehydration, salt, cold and ABA stress. Over-expression of ZmABI3-L in Arabidopsis plants could enhance salt and drought stress tolerance compared to the wild type. The results suggest that ZmABI3-L may be involved in salt and drought resistance signaling pathways in maize.

袁进成, 宋晋辉, 马海莲, 瓮巧云, 王凌云, 赵艳, 刘颖慧. 转玉米ZmABI3-L基因增加拟南芥的抗旱和耐盐性[J]. 草业学报, 2016, 25(2): 124-131.

YUAN Jin-Cheng, SONG Jin-Hui, MA Hai-Lian, WENG Qiao-Yun, WANG Ling-Yun, ZHAO Yan, LIU Ying-Hui. A maize abscisic acid insensitive 3 gene confers drought and salt stress tolerance in Arabidopsis[J]. Acta Prataculturae Sinica, 2016, 25(2): 124-131.

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转玉米ZmABI3-L基因增加拟南芥的抗旱和耐盐性

A maize abscisic acid insensitive 3 gene confers drought and salt stress tolerance in Arabidopsis

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