利用SSR标记分析高粱属种质资源的遗传多样性

doi:10.11686/cyxb2015336

草业学报 ›› 2016, Vol. 25 ›› Issue (5): 125-133.DOI: 10.11686/cyxb2015336

利用SSR标记分析高粱属种质资源的遗传多样性

王芳^{1**, *}, 高秋^{2**, *}, 王杰¹, 马金星², 孙娟^{1*, *}

1.青岛农业大学经济草本植物应用研究所,山东青岛 266109;
2.农业部全国草产品质量监督检验测试中心,北京 100125

收稿日期:2015-07-07 出版日期:2016-05-20 发布日期:2016-05-20
通讯作者: *通信作者Corresponding author. E-mail: sunjuan@qau.edu.cn
作者简介:王芳(1990-),女,山东济宁人,在读硕士。E-mail: wangfang213097056@163.com。高秋(1980-),女,云南保山人,畜牧师。E-mail:gaoqiu1980@gmail.com。
基金资助:
国家牧草产业技术体系项目资助; 作者简介:王芳(1990-),女,山东济宁人,在读硕士; E-mail: wangfang213097056@163; com; 高秋(1980-),女,云南保山人,畜牧师; E-mail:gaoqiu1980@gmail; com

Analysis of genetic diversity in Sorghum germplasm collections using SSR markers

WANG Fang^{1, **}, GAO Qiu^{2, **}, WANG Jie¹, MA Jin-Xing², SUN Juan^{1, *}

1.Applied Economic Herbaceous Plants Institute of Qingdao Agricultural University, Qingdao 266109, China;
2.National Quality Control & Inspection Centre For Grassland Industry Products, M.O.A, Beijing 100125, China

Received:2015-07-07 Online:2016-05-20 Published:2016-05-20

摘要/Abstract

摘要： 本研究利用15对SSR 引物对161 份高粱属种质资源进行了遗传多样性分析。结果显示,15对引物共扩增出118条谱带,其中88条具有多态性,多态性比率(P)为75.21%;多态性信息含量(PIC值)变幅为0.612~0.806,平均为0.699。161份高粱属材料遗传相似性系数为0.636~0.977,平均基因多样性指数(H)为0.696,Shannon信息指数(I)为1.393。UPGMA聚类分析显示, 161 份高粱属材料在遗传相似系数(GS)为0.682处可分为3组;129份高粱和苏丹草材料在相似系数为0.671处可分为2组。表明SSR标记可以作为高粱属种间以及种内遗传分化分析的有力工具,被分析的高粱属种质材料具有较高的遗传多样性。

Abstract: Fifteen SSR markers were used to quantify the genetic diversity of the core germplasm collections of sorghum. A total of 161 accessions were assayed. 118 bands were generated, among which 88 (75.21%) were polymorphic. The average value of polymorphic information content (PIC) was 0.699 (0.612-0.806); the average Nei’s gene diversity index (H) was 0.696; and the average Shannon information index (I) was 1.393. The genetic similarities among the 161 accessions ranged from 0.636 to 0.977. The UPGMA dendrogram indicated that the 161 Sorghum germplasm collections were clustered into three groups with a threshold of 0.682, and that the 129 Sorghum sudanense and Sorghum bicolorx×Sorghum sudanense germplasm resources were divided into two clusters at a threshold of 0.671. This study demonstrated that there is considerable genetic diversity conserved in Sorghum germplasm collections and SSR is an ideal molecular marker for assessing this diversity.

王芳, 高秋, 王杰, 马金星, 孙娟. 利用SSR标记分析高粱属种质资源的遗传多样性[J]. 草业学报, 2016, 25(5): 125-133.

WANG Fang, GAO Qiu, WANG Jie, MA Jin-Xing, SUN Juan. Analysis of genetic diversity in Sorghum germplasm collections using SSR markers[J]. Acta Prataculturae Sinica, 2016, 25(5): 125-133.

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利用SSR标记分析高粱属种质资源的遗传多样性

Analysis of genetic diversity in Sorghum germplasm collections using SSR markers

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