紫花苜蓿不同品种对拟枝孢镰刀菌的抗性评价

doi:10.11686/cyxb2015331

草业学报 ›› 2016, Vol. 25 ›› Issue (5): 95-101.DOI: 10.11686/cyxb2015331

紫花苜蓿不同品种对拟枝孢镰刀菌的抗性评价

潘龙其^{1, 2}, 张丽¹, 田进山², 袁庆华^{1*, *}, 王瑜¹, 苗丽宏¹

1.中国农业科学院北京畜牧兽医研究所,北京 100193;
2.宜昌市农业科学研究院,湖北宜昌 443004

收稿日期:2015-06-30 出版日期:2016-05-20 发布日期:2016-05-20
通讯作者: *通信作者Corresponding author. E-mail: yuanqinghua@iascaas.net.cn
作者简介:作者简介:潘龙其(1988-),男,甘肃天水人,在读硕士。 E-mail:gsaupanlongqi@126.com
基金资助:
公益性行业(农业)科研专项项目(201303057)资助

ResistanceevaluationofdifferentalfalfavarietiestoFusariumsporotrichioides

PAN Long-Qi^{1, 2}, ZHANG Li¹, TIAN Jin-Shan², YUAN Qing-Hua^{1, *}, WANG Yu¹, MIAO Li-Hong¹

1.InstituteofAnimalScienceofCAAS,Beijing 100193,China;
2.YichangAcademyofAgriculturalSciences,Yichang 443004,China

Received:2015-06-30 Online:2016-05-20 Published:2016-05-20

摘要/Abstract

摘要： 在温室盆栽30个紫花苜蓿品种45 d后,将拟枝孢镰刀菌接种于幼苗根部土壤中,10 d根据根部病情指数评定抗病性,筛选抗性后选择抗病和感病品种再次接种,于4,8和12 h时测定与抗性相关酶的活性。结果表明,不同品种间抗性差异显著,其中耐病品种为晋南苜蓿、驯鹿、PG塞特、苏联二号4 个,占测定品种总数的13.3%;高感品种为新疆大叶、中苜一号、陇中苜蓿、北疆苜蓿、皇冠、甘肃苜蓿和萨兰斯7个,占23.3%;其余的均为感病品种,占63.4%;未发现抗病和免疫品种。酶活性变化测定表明,对于接菌与未接菌的供试耐病和高感苜蓿品种,不同品种间CAT、SOD、PAL的活性存在一定差异;总体接菌植株的酶活性高于对照,且耐病品种的酶活性高于感病品种,酶活性在接菌后4~8 d呈上升趋势,8~12 d呈下降趋势;接菌植株CAT活性较对照增幅明显,SOD、PAL活性较其对照增幅不明显。

Abstract: The present study evaluated the resistance of 30 alfalfa varieties to Fusariumsporotrichioides by inoculation of 45 d old seedlings in a greenhouse. Resistant and susceptible varieties were selected based on the root disease index after 10 days of inoculation and were further evaluated by inoculation pathogen and measuring enzymes activities after 4,8 and 12 hours inoculation. The results showed that there were four resistant varieties: Jinnan, Xunlu, PG Saite and Sulian No.2, which together accounted for 13.3%. There were seven highly sensitive varieties: Xinjiangdaye, Zhongmu No.1, Longzhong, Beijiang, Huangguan, Gansu and Salansi, which together accounted for 23.3%. The remaining varieties were all sensitive and together accounted for 63.4%. There were no resistant or immune varieties. Measurement of enzyme activities showed highly significant variations in CAT, SOD and PAL activities among the different varieties, for both the inoculated samples and for the controls without inoculation. Enzyme activities in inoculated plants were higher than those of control plants and enzyme activities in the resistant varieties were higher than those of susceptible varieties. Enzyme activity increased after 4-8 d inoculation and decreased after 8-12 d inoculation. CAT activity of the inoculated plants significantly increased compared to the control, while SOD and PAL activities did not significantly increase.

潘龙其, 张丽, 田进山, 袁庆华, 王瑜, 苗丽宏. 紫花苜蓿不同品种对拟枝孢镰刀菌的抗性评价[J]. 草业学报, 2016, 25(5): 95-101.

PAN Long-Qi, ZHANG Li, TIAN Jin-Shan, YUAN Qing-Hua, WANG Yu, MIAO Li-Hong. ResistanceevaluationofdifferentalfalfavarietiestoFusariumsporotrichioides[J]. Acta Prataculturae Sinica, 2016, 25(5): 95-101.

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紫花苜蓿不同品种对拟枝孢镰刀菌的抗性评价

ResistanceevaluationofdifferentalfalfavarietiestoFusariumsporotrichioides

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