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草业学报 ›› 2013, Vol. 22 ›› Issue (2): 227-234.

• 研究论文 • 上一篇    下一篇

低温胁迫5种菌草的抗寒性评价

林兴生1,3,林占熺1,3*,林冬梅1,3,林辉1,3,罗海凌1,3,胡应平1,3,林春梅1,3,朱朝枝2*   

  1. 1.福建农林大学菌草研究所,福建 福州350002;
    2.福建农林大学金山学院,福建 福州350002;
    3.国家菌草工程技术研究中心, 福建 福州350002
  • 收稿日期:2011-12-27 出版日期:2013-02-25 发布日期:2013-04-20
  • 通讯作者: E-mail:singelin@163.com
  • 作者简介:林兴生(1973-),男,福建连城人,副研究员,在读博士。E-mail:wittylin@163.com
  • 基金资助:
    国家林业公益性行业科研专项(201004019),科技部国家中心组建项目(2011FU125X11)和福建省科技重大专项(2012NZ0002)资助。

Cold-tolerance of 5 species of Juncao under low temperature stress

LIN Xing-sheng1,3, LIN Zhan-xi1,3, LIN Dong-mei1,3, LIN Hui1,3, LUO Hai-ling1,3, HU Ying-ping1,3, LIN Chun-mei1,3, ZHU Chao-zhi2   

  1. 1.Juncao Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2.Jinshan College, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    3.National Engineering Research Center of Juncao, Fuzhou 350002, China
  • Received:2011-12-27 Online:2013-02-25 Published:2013-04-20

摘要: 研究莱竹、芦竹、巨菌草、象草、稗草等5种菌草的抗寒能力,分别以3,0,-5,-10,-15,-20℃等不同的温度处理,模拟低温胁迫,测定电解质渗透率、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、丙二醛(MDA)含量、可溶性糖(SS)含量、超氧化物歧化酶(SOD)活性等生理生化指标及恢复试验后的死亡率,结果表明,莱竹、芦竹、巨菌草、象草、稗草等5种菌草的半致死温度LT50分别为:-12.05,-21.28,0.69,0.18,-1.98℃;通过隶属函数法对各生理指标进行综合分析,5种菌草抗寒能力强弱为芦竹>莱竹>稗草>象草>巨菌草,该结果与恢复试验一致。

Abstract: To select excellent Juncao species with high cold-resistance, 5 species of Juncao including Arundo sp., Arundo donax, Pennisetum sp., Pennisetum purpureum and Echinochloa crusgalli were taken as test materials and treated at 3, 0, -5, -10, -15, -20℃. Electrolyte leakage, malondialdehyde content and soluble sugar content, activity of superoxide dismutase, peroxidase, catalase and the mortality rate after recovery test were determined to detect cold resistance of the 5 species. The semi-lethal temperatures (LT50) of Arundo sp., A. donax, Pennisetum sp., P. purpureum and E. crusgalli were -12.05, -21.28, 0.69, 0.18, -1.98℃ respectively. The cold-resistance of the 5 Juncao species was evaluated mathematically as A. donax >Arundo sp.>E. crusgalli>P. purpureum>Pennisetum sp., which is in accord with the results after the recovery test from low temperature stress.

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