青海箭筈豌豆根瘤菌的筛选及其共生体耐盐性研究

doi:10.11686/cyxb2015497

草业学报 ›› 2016, Vol. 25 ›› Issue (8): 145-153.DOI: 10.11686/cyxb2015497

青海箭筈豌豆根瘤菌的筛选及其共生体耐盐性研究

王雪翠^{1, 2*, *}, 马晓彤^{1*, *}, 韩梅^{3*, *}, 曹卫东^{1, 3*, *}, 张宏亮³, 白金顺¹, 曾闹华¹, 高嵩涓^{1, 2}, 周国朋^{1, 2}, 王艳秋⁴

1.中国农业科学院农业资源与农业区划研究所,农业部植物营养与肥料重点实验室,北京 100081;
2.中国农业科学院研究生院,北京 100081;
3.青海大学青海省农林科学院土壤肥料研究所,青海西宁 8110016;
4.内蒙古师范大学,内蒙古呼和浩特 010022

收稿日期:2015-11-03 修回日期:2015-12-28 出版日期:2016-08-20 发布日期:2016-08-20
通讯作者: caoweidong@caas.cn
作者简介:王雪翠(1989-),女,山东济宁人,在读硕士。E-mail: wangxuecui613@163.com。马晓彤(1967-),女,北京人,助理研究员。E-mail: maxiaotong@caas.cn。韩梅(1973-),女,青海湟中人,副研究员。E-mail: hanmei20061234@sina.com。**共同第一作者
基金资助:
公益性行业(农业)科研专项(201103005),中国农业科学院科技创新工程,国家农作物种质资源平台运行服务项目(NICGR2015-019),农作物种质资源保护与利用专项(2015NWB042)和青海省“昆仑学者”计划资助

Screening of rhizobia of common vetch (Vicia sativa) in Qinghai, and assessment of symbiont salt tolerance

WANG Xue-Cui^{1, 2, **}, MA Xiao-Tong^{1, **}, HAN Mei^{3, **}, CAO Wei-Dong^{1, 3, *}, ZHANG Hong-Liang³, BAI Jin-Shun¹, ZENG Nao-Hua³, GAO Song-Juan^{1, 2}, ZHOU Guo-Peng^{1, 2}, WANG Yan-Qiu⁴

1.Key laboratory of Plant Nutrition and Fertilizer,Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing00081,China;
2.Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China;
3.Soil and Fertilizer Institute,Qinghai Academy of Agriculture and Forestry Sciences,Qinghai University,Xining 810016,China;
4.Inner Mongolia Normal University,Hohhot 010022,China

Received:2015-11-03 Revised:2015-12-28 Online:2016-08-20 Published:2016-08-20

摘要/Abstract

摘要： 箭筈豌豆是我国传统肥饲兼用的豆科绿肥作物,有关箭筈豌豆根瘤菌的研究尚属空白。为获得抗逆性强的根瘤菌菌株,在青海主产区采集样本,经分离、鉴定得到61株箭筈豌豆根瘤菌。采用水培回接法,通过主成分分析筛选出5株对宿主结瘤生长促进作用较强的根瘤菌(J1-3-2、J2-6-2、J3-12-1、J4-3-3和J5-5-3)。对该5株根瘤菌进行了离体耐盐性评价,发现菌株J5-5-3最大耐盐极限值在0.9%左右,其他4菌株最大耐盐极限值均在1.2%左右,并且5菌株的耐盐特征呈3个明显不同类型:J3-12-1和J2-6-2耐盐性最强,菌株J1-3-2和J4-3-3居中,菌株J5-5-3最差。进一步采用盆栽方法评价了其中3株(每类型1株)根瘤菌-箭筈豌豆共生体的耐盐性,结果表明,在0.15%NaCl胁迫下接种根瘤菌J5-5-3植株结瘤数最多,在0.30%NaCl胁迫下3种接种处理无显著差异;在0.15%和0.30%NaCl胁迫下,接种J3-12-1共生体株高显著大于对照(P<0.05);在0.15%和0.30%NaCl胁迫下根瘤菌J3-12-1和J5-5-3可显著提高植株干重(P<0.05);J5-5-3在0.15%和0.30%NaCl胁迫下可显著提高植株含氮量并显著提高0.15%NaCl胁迫下的植株吸氮量(P<0.05);J3-12-1显著提高了0.15%NaCl胁迫下的植株吸氮量(P<0.05)。综合来看,J3-12-1和J5-5-3可以作为在盐胁迫下提高箭筈豌豆生物量和含氮量的预选菌株。

Abstract: Common Vetch (Vicia sativa) is a traditional legume crop in China which is widely used as a green manure and for fodder. However, there has been little study to date of the rhizobia of common vetch. This study aimed to identify rhizobial strains of common vetch with superior symbiotic properties, and also greater salt tolerance. Root nodule samples were collected from the major production areas of common vetch in Qinghai, and 61 rhizobial strains were isolated and identified. Using a hydroponics inoculation technique and principal component analysis, 5 strains with stronger ability to promote nodulation and growth of the host plants were identified, and designated J1-3-2, J2-6-2, J3-12-1, J4-3-3 and J5-5-3. The salt tolerance of these 5 strains was examined by single plant culture. Strain J5-5-3 was tolerant of NaCl concentrations up to about 0.9%, while the other 4 strains could tolerate up to 1.2% NaCl concentration. Three distinct levels of salt tolerance were evident: J3-12-1 and J2-6-2 displayed the strongest tolerance, J1-3-2 and J4-3-3 displayed medium tolerance, and J5-5-3 the weakest tolerance. One strain from each of these groups was then evaluated in a pot experiment to asses nodulation of the host under differing salinity levels of 0.15% or 0.3% NaCl. When exposed to 0.15% NaCl concentration, the largest number of root nodules was observed in plants inoculated with J5-5-3, and no significant differences among the three rhzobial strains were found when exposed to 0.30% NaCl concentration. J3-12-1 and J5-5-3 inoculation resulted in increased dry weight (P<0.05) of the host plants under NaCl concentrations of 0.15% and 0.30%. Inoculation with strain J5-5-3 increased plant nitrogen content (P<0.05) under the two levels of NaCl stress and increased nitrogen uptake (P<0.05) under the 0.15% NaCl stress, while the strain J3-12-1 significantly increased the nitrogen uptake (P<0.05) of the host plants under 0.15% NaCl stress. It was concluded that the strains J3-12-1 and J5-5-3 are suitable as inoculant rhizobial strains to increase the biomass and nitrogen content of common vetch under salt stress.

王雪翠, 马晓彤, 韩梅, 曹卫东, 张宏亮, 白金顺, 曾闹华, 高嵩涓, 周国朋, 王艳秋. 青海箭筈豌豆根瘤菌的筛选及其共生体耐盐性研究[J]. 草业学报, 2016, 25(8): 145-153.

WANG Xue-Cui, MA Xiao-Tong, HAN Mei, CAO Wei-Dong, ZHANG Hong-Liang, BAI Jin-Shun, ZENG Nao-Hua, GAO Song-Juan, ZHOU Guo-Peng, WANG Yan-Qiu. Screening of rhizobia of common vetch (Vicia sativa) in Qinghai, and assessment of symbiont salt tolerance[J]. Acta Prataculturae Sinica, 2016, 25(8): 145-153.

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青海箭筈豌豆根瘤菌的筛选及其共生体耐盐性研究

Screening of rhizobia of common vetch (Vicia sativa) in Qinghai, and assessment of symbiont salt tolerance

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