盐碱胁迫下新疆野豌豆种子萌发及幼苗生理响应

doi:10.11686/cyxb2016023

摘要/Abstract

摘要： 以新疆野豌豆为材料,研究不同浓度NaCl(0,50,100,150和200 mmol/L)和Na₂CO₃ (0,10,20,30,40 mmol/L)胁迫对种子萌发和幼苗生理状态的影响。结果表明, 1)随着两种胁迫浓度的升高,种子萌发率呈下降趋势,其中Na₂CO₃胁迫下种子萌发率下降幅度显著高于NaCl胁迫; 2)两种胁迫条件下丙二醛和脯氨酸含量均呈上升趋势,Na₂CO₃胁迫上升幅度明显大于NaCl胁迫; 3)两种胁迫下超氧化物歧化酶(SOD)和过氧化物酶(POD)活性均表现出先升高后降低的趋势,其中Na₂CO₃胁迫降低幅度稍大;4)可溶性糖含量在Na₂CO₃胁迫下呈升高趋势,而NaCl胁迫则表现出先升高后降低的趋势。该研究结果显示碱性盐(Na₂CO₃)胁迫对新疆野豌豆萌发和生长的抑制作用显著高于中性盐(NaCl)胁迫,此结论为新疆野豌豆的品种选育和推广种植提供一定的理论指导和技术参考。

Abstract: The effects of NaCl (0, 50, 100, 150, 200 mmol/L) and Na₂CO₃ (0, 10, 20, 30, 40 mmol/L) stresses on seed germination and early seedling metabolism were studied in Vicia costata. Parameters measured included: soluble sugar, proline, malondialdehyde, and superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzyme activities. Key results were: 1) Seed germination rates were significantly reduced under both types of stress, but germination reduction under Na₂CO₃ stress was much greater than under NaCl stress; 2) Concentrations of MDA and proline increased under both kinds of stress, but increases associated with Na₂CO₃ stress were much greater than with NaCl stress; 3) The activities of SOD and POD were increased at low stress and decreased at high stress, but the decease under high NaCl stress was small; 4) The soluble sugar concentration was increased under Na₂CO₃ stress, but under NaCl stress was increased at low stress and decreased at high stress. In summary, the inhibition of Vicia costata under Na₂CO₃ stress is much greater than under NaCl stress. This research provides data about this forage species that will assist with the formulation of crop husbandry guidelines.

李莉, 张一弓, 贾纳提, 李学森. 盐碱胁迫下新疆野豌豆种子萌发及幼苗生理响应[J]. 草业学报, 2016, 25(9): 46-53.

LI Li, ZHANG Yi-Gong, JIANATI, LI Xue-Sen. Seed germination and physiological responses of Vicia costata under saline-alkali stress conditions[J]. Acta Prataculturae Sinica, 2016, 25(9): 46-53.

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