盐胁迫对菊芋糖组分含量和分配的影响

doi:10.11686/cyxb2016227

草业学报 ›› 2017, Vol. 26 ›› Issue (5): 127-134.DOI: 10.11686/cyxb2016227

盐胁迫对菊芋糖组分含量和分配的影响

詹文悦^{1, 2}, 李辉^{1, 2}, 康健^{1, 2}, 尹晓明¹, 梁明祥^{1, 2, *}

1.南京农业大学资源与环境科学学院,江苏南京 210095；
2.江苏省海洋生物学重点实验室,江苏南京 210095

收稿日期:2016-05-27 修回日期:2016-07-06 出版日期:2017-05-20 发布日期:2017-05-20
作者简介:詹文悦(1994-),女,安徽池州人,在读硕士。E-mail: 2015803171@njau.edu.cn
基金资助:
江苏省科技项目-社会发展类(BE2015681)和国家自然科学基金(31201842)资助

Effect of salt stress on the accumulation and distribution of sugars in Jerusalem artichoke (Helianthus tuberosus) plants

ZHAN Wen-Yue^{1, 2}, LI Hui^{1, 2}, KANG Jian^{1, 2}, YIN Xiao-Ming¹, LIANG Ming-Xiang^{1, 2, *}

1.College of Resource and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China;
2.Key Discipline of Marine Biology, Nanjing Agricultural University, Nanjing 210095, China

Received:2016-05-27 Revised:2016-07-06 Online:2017-05-20 Published:2017-05-20

摘要/Abstract

摘要： 本研究以菊芋品种南芋一号(NY-1)为试材,在温室进行全生育期土培盆栽试验,研究了盐胁迫对糖分积累与分配的影响。通过HPLC-ELSD(高效液相色谱-蒸发光检测仪)检测发现,盐胁迫对NY-1块茎、茎和叶中各种糖的含量有不同影响。对于单糖和二糖来说,与对照相比,盐处理140 DAP (days after planting)时茎中的果糖、葡萄糖和蔗糖含量分别降低了72.67%,78.31%和39.40%;块茎中果糖、葡萄糖和蔗糖含量分别降低了67.08%,60.91%和30.07%;叶中果糖、葡萄糖和蔗糖含量与对照相比无显著性变化。盐处理220 DAP时,块茎中这些糖的含量无明显降低,茎中的葡萄糖含量与对照相比降低了139.22%;而叶中这些糖已检测不出。低聚合度果聚糖主要集中分布在菊芋块茎和茎中。与对照相比,盐处理140 DAP 时茎与块茎中蔗果三糖、蔗果四糖和蔗果五糖的含量没有显著差异,叶中这些糖分布很少,与对照相比变化很小。盐处理220 DAP,块茎中这些糖含量与对照相比无显著性差异,而茎与对照相比,蔗果三糖、蔗果四糖和蔗果五糖含量几乎为0,表明随块茎的形成,茎和叶中的果聚糖逐步向块茎转运,盐胁迫产生渗透机制,增加了果聚糖在块茎中的积累。以上结果表明盐胁迫影响了糖分的积累转运,微调了植物体内的果聚糖组成,但极其严重地减少了果聚糖在地上部分(尤其是茎)的积累。

Abstract: A pot experiment was conducted to determine the effects of salinity on the accumulation and distribution of sugars in Jerusalem artichoke (Helianthus tuberosus cv. Nanyu No. 1). The tuber, stem, and leaf showed differences in sugar accumulation under salt stress, as determined by high-performance liquid chromatography coupled with evaporative light scattering detection. At 140 DAP (days after planting), the contents of fructose, glucose, and sucrose were 72.67%, 78.31%, and 39.40% lower, respectively, in the stems of salt-treated plants than in those of control plants; and 67.08%, 60.91%, and 30.07% lower, respectively, in the tubers of salt-treated plants than in those of control plants. The contents of fructose, glucose, and sucrose in the leaves did not differ significantly between salt-treated and control plants. At 220 DAP, the contents of fructose, glucose, and sucrose had hardly changed in the tuber, but the glucose content in the stem was 139.22% lower in the salt-treated plants than in the control plants, and sugars were not detected in the leaves at this stage. Generally, there was a low degree polymerization of fructans in the tubers and stems of Jerusalem artichoke. Compared with the control, the salt-treated plants at 140 DAP showed different contents of 1-kestose, nystose, and 1F-fructofuranosylnystose in the tuber and stem. The sugar content in leaves was very low in salt-treated plants at this stage, similar to that in the control. At 220 DAP, 1-kestose, nystose, and 1F-fructofuranosylnystose were barely detectable in the stems of salt-treated plants, while the contents of 1-kestose and 1F-fructofuranosylnystose in the tubers had not changed significantly. The results implied that sugars in the stem and leaves were gradually transferred to the tuber as it developed. Salt stress induced an osmotic adjustment followed by sugar accumulation in the tuber. Salt stress affected the accumulation and distribution profiles of sugars in Jerusalem artichoke, and greatly reduced fructan accumulation in aboveground tissues, especially the stem.

中图分类号:

liangmx@njau.edu.cn

詹文悦, 李辉, 康健, 尹晓明, 梁明祥. 盐胁迫对菊芋糖组分含量和分配的影响[J]. 草业学报, 2017, 26(5): 127-134.

ZHAN Wen-Yue, LI Hui, KANG Jian, YIN Xiao-Ming, LIANG Ming-Xiang. Effect of salt stress on the accumulation and distribution of sugars in Jerusalem artichoke (Helianthus tuberosus) plants[J]. Acta Prataculturae Sinica, 2017, 26(5): 127-134.

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盐胁迫对菊芋糖组分含量和分配的影响

Effect of salt stress on the accumulation and distribution of sugars in Jerusalem artichoke (Helianthus tuberosus) plants

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