体外法评价玉米秸秆、谷草和玉米秸秆青贮饲料组合效应研究

doi:10.11686/cyxb2016276

草业学报 ›› 2017, Vol. 26 ›› Issue (5): 213-223.DOI: 10.11686/cyxb2016276

体外法评价玉米秸秆、谷草和玉米秸秆青贮饲料组合效应研究

李妍^1,*, 韩肖敏^2,*, 李建国², 李秋凤², 高艳霞², 曹玉凤^{2, *}, 李运起²

1.河北农业大学动物医学院,河北保定 071001；
2.河北农业大学动物科技学院,河北保定 071001

收稿日期:2016-07-14 修回日期:2016-09-07 出版日期:2017-05-20 发布日期:2017-05-20
作者简介:李妍(1987-),女,河北保定人,博士。E-mail: 239662307@qq.com。韩肖敏(1989-),女,河北邯郸人,硕士。E-mail:1031307070@qq.com.
基金资助:
公益性行业(农业)科研专项(201503134),河北省科技计划项目(16226604D)和国家现代农业产业技术体系建设专项资金资助

Associative effects of cornstalk, millet straw, and corn stalk on silage digestibility in vitro

LI Yan^1,*, HAN Xiao-Min^2,*, LI Jian-Guo², LI Qiu-Feng², GAO Yan-Xia², CAO Yu-Feng^{2, *}, LI Yun-Qi²

1.College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China;
2.College of Animal Science and Technology, Hebei Agricultural University, Baoding 071001, China

Received:2016-07-14 Revised:2016-09-07 Online:2017-05-20 Published:2017-05-20

摘要/Abstract

摘要： 本试验旨在利用体外产气法研究玉米秸秆(CS)、谷草(MS)与玉米秸秆青贮饲料(CSS)间的组合效应,筛选出不同饲料间的适宜组合比例。试验采用单因素试验设计,首先将玉米秸秆与谷草进行体外发酵试验,筛选出的最优组合,再与玉米秸秆青贮饲料组合进行试验,以上组合均以100∶0,80∶20,60∶40,50∶50,40∶60,20∶80,0∶100的比例进行,每个组合设3个重复,测定和分析不同粗饲料组合对体外产气量、干物质消失率、pH、微生物蛋白、氨态氮、挥发性脂肪酸的影响,计算出各组合的单项组合效应值和综合组合效应值。试验结果表明,不同饲料组合对产气量和干物质消失率影响显著或极显著(P<0.05或P<0.01);不同饲料组合间pH差异不显著(P>0.05);各饲料组合间微生物蛋白产量差异显著或极显著(P<0.05或P<0.01),玉米秸秆与谷草组合的微生物蛋白产量随玉米秸秆比例的减少而减少,再与玉米秸秆青贮饲料组合随玉米秸秆青贮饲料比例的增加而增加;不同饲料组合的氨态氮浓度差异显著或极显著(P<0.05或P<0.01),含量为17.35~24.63 mg/dL;各饲料组合间的挥发性脂肪酸产量差异显著或极显著(P<0.05或P<0.01)。以多项组合效应指数评定的最优组合为:玉米秸秆和谷草的搭配比例为60∶40,玉米秸秆、谷草、玉米秸秆青贮饲料的搭配比例为12∶8∶80。

Abstract: We investigated the associative effects of cornstalk (CS), millet straw (MS), and corn stalk silage (CSS) on silage digestibility using an in vitro rumen fermentation system. A single factor experiment was designed with CS and MS to identify the best CS-MS combination, then CSS was added in combination with CS-MS. First, CS and MS were tested at ratios of 100∶0, 80∶20, 60∶40, 50∶50, 40∶60, 20∶80 and 0∶100, with three replicates. Gas production (GP), rate of dry matter loss (DML), pH, microbial crude protein (MCP), ammonia nitrogen (NH₃-N), and volatile fatty acids (VFA) were measured for all the CS-MS combinations, and the single factor associative effects index and multiple factors associative effects index of different combinations were calculated. The results showed that GP and DML differed among the various combinations significantly (P<0.05) or extremely significantly (P<0.01). However, pH did not differ among the various combinations (P>0.05). The MCP yield differed significantly (P<0.05) or extremely significantly (P<0.01) among the different CS-MS combinations. The MCP yields of CS-MS combinations decreased with decreasing proportions of CS, while the MCP yields of the CS-MS-CSS (CS∶MS=60∶40) combinations increased with increasing proportions of CSS. There were significant (P<0.05) or extremely significant (P<0.01) differences in NH₃-N concentrations among the various combinations, ranging from 17.35 mg/dL to 24.63 mg/dL. There were significant (P<0.05) or extremely significant differences (P<0.01) of VFA yield among the various combinations. In conclusion, the CS-MS ratio of 60∶40 and the CS-MS-CSS combination at the ratio of 12∶8∶80 produced the largest associative effects in this study.

中图分类号:

cyf278@sohu.com

李妍, 韩肖敏, 李建国, 李秋凤, 高艳霞, 曹玉凤, 李运起. 体外法评价玉米秸秆、谷草和玉米秸秆青贮饲料组合效应研究[J]. 草业学报, 2017, 26(5): 213-223.

LI Yan, HAN Xiao-Min, LI Jian-Guo, LI Qiu-Feng, GAO Yan-Xia, CAO Yu-Feng, LI Yun-Qi. Associative effects of cornstalk, millet straw, and corn stalk on silage digestibility in vitro[J]. Acta Prataculturae Sinica, 2017, 26(5): 213-223.

参考文献

[1] Li S S, Dong H R, Li X, et al . Analysis of the utilization of agricultural crops straws and influence factors in Hebei Province. Heilongjiang Animal Husbandry and Veterinary, 2015, (3): 6-8.
李珊珊, 董海荣, 李霞, 等. 河北省农作物秸秆利用现状及其影响因素分析. 黑龙江畜牧兽医, 2015, (3): 6-8.
[2] Wang H Q. The ministry of agriculture organization had completed the first special investigation of national crop straw resource. Rural Know-all, 2011, (3): 10.
王洪芹. 农业部组织完成首次全国农作物秸秆资源专项调查. 农村百事通, 2011, (3): 10.
[3] Jiang P. The nutrient trait of straw feed and its utilization. Sichuan Animal and Veterinary Sciences, 2004, 31(11): 24-25.
江平. 秸秆饲料的营养特点及利用. 四川畜牧兽医, 2004, 31(11): 24-25.
[4] Guo Y Q, Zhang Y J, Cheng S Y, et al . The nutritional characteristics, development and utilization of millet straw. Henan Journal of Animal Husbandry and Veterinary Medicine, 2009, 30(12): 31-32.
郭勇庆, 张英杰, 程善燕, 等. 谷草的营养特点及开发利用. 河南畜牧兽医, 2009, 30(12): 31-32.
[5] Lu D X. Animal’s self-regulation theory and its application. Inner Mongolia Animal Husbandry Science, 1995, 1: 1-10.
卢德勋. 动物机体自我调控理论及其应用.内蒙古畜牧科学, 1995, 1: 1-10.
[6] Liu J X, Susenbeth A, Stidekum K H. In vitro gas production measurements to evaluate interactions between untreated and chemically treated ricestraws, grass hay, and mulberry leaves. Journal of Animal Science, 2002, (80): 517-524.
[7] Wu Y M, Liu J X. The research progress of associative effect between ruminant feed. Animal Husbandry and Veterinary, 2002, (34): 113-117.
吴越明, 刘建新. 反刍动物饲料间组合效应的研究进展. 畜牧与兽医, 2002, (34): 113-117.
[8] Lu D X. Associative effect of feed[M]//Zhang Z Y. Chinese Feed Science. Beijing: China Agriculture Press, 2000.
卢德勋. 饲料的组合效应[M]//张子仪. 中国饲料学. 北京: 中国农业出版社, 2000.
[9] Yang L. Study on the Associative Effects of Alfalfa, Cornstalk and Concentrate Supplement in Cattle Diets[D].Changchun: Jilin Agricultural University, 2007.
杨丽. 肉牛日粮中苜蓿、玉米秸秆、精料补充料组合效应研究[D]. 长春: 吉林农业大学, 2007.
[10] Tang S Y. Evaluation of Associative Effects between Corn Silage and Rice Straw[D]. Haerbin: Northeast Agricultural University, 2009.
唐赛涌. 青贮玉米和稻秸之间的组合效应研究[D]. 哈尔滨: 东北农业大学, 2009.
[11] Cui Z H, Liu S J, Chai S T, et al . Evaluation of associate effects of alfalfa greenhay and crops straws in qinghai plateau. Acta Agriculturae Boreali-occidentalis Sinica, 2012, 21(2): 146-152.
崔占鸿, 刘书杰, 柴沙驼, 等. 青海高原苜蓿青干草与农作物秸秆组合效应评价. 西北农业学报, 2012, 21(2): 146-152.
[12] Sun G Q, Lv Y Y, Zang J J. A study on the associative effect of whole corn silage-peanut vine and Leymus chinensis by rumen fermentation in vitro . Acta Prataculturae Sinica, 2014, 23(3): 224-231.
孙国强, 吕永艳, 张杰杰. 利用体外瘤胃发酵法研究全株玉米青贮与花生蔓和羊草间的组合效应. 草业学报, 2014, 23(3): 224-231.
[13] Qiu X, Wang X G, Liu Y H, et al . The feeding effects of sunit lamb using different forage combinations in desert steppe. Animal Husbandry and Feed Science, 2012, (C2): 104-107.
邱晓, 王晓光, 刘亚红, 等. 荒漠草原区不同饲草组合对苏尼特羔羊饲喂效果研究. 畜牧与饲料科学, 2012, (C2): 104-107.
[14] Kiran D, Krishnamoorthy U. Rumen fermentation and microbial biomass synthesis indices of tropical feedstuffs determined by the in virto gas production technique. Animal Feed Science and Technology, 2007, 134(1/2): 170-179.
[15] Zhang L Y. Technique of Feed Analysis and Quality Testing[M]. Beijing: China Agricultural University Press, 2010: 45-63.
张丽英. 饲料分析及饲料质量检测技术[M]. 北京: 中国农业大学出版社, 2010: 45-63.
[16] Goering H K, VanSoest P J. Forage Fiber Analysis (apparatus, reagents, procedures and some applications)[M]. U. S. Government Printing Office: Washington, DC. USDA-ARS Agricultural Handbook, 1970: 379.
[17] Feng Z C, Gao M. The colorimetry method improvement of rumen ammonia nitrogen content is determined. Inner Mongolia Animal Husbandry Science, 1993, (4): 40-41.
冯宗慈, 高民. 通过比色法测定瘤胃液氨氮含量方法的改进. 内蒙古畜牧科学, 1993, (4): 40-41.
[18] Cotta M A, Russell J R. Effect of peptides and amino acids on efficiency of rumen bacterial protein synthesis in continuous culture. Journal of Dairy Science, 1982, 65: 226-234.
[19] Wang J Q. The Research Methods of Ruminant Nutrition[M]. Beijing: Modern Education Press, 2011.
王加启. 反刍动物营养学研究方法[M]. 北京: 现代教育出版社, 2011.
[20] Wang X. A Technique for Formulation of Mixed Forages by Grading Index and Systematic Optimization of Sheep Ration based on the Technique[D]. Hohhot: Inner Mongolia Agriculture University, 2003.
王旭. 利用GI 技术对粗饲料进行科学搭配及绵羊日粮配方系统优化技术的研究[D]. 呼和浩特: 内蒙古农业大学, 2003.
[21] Lei D Z, Jin S G, Wuren T N. Evaluation of the association effects of same concentration and different forages by gas production method in vitro . Feed Industry, 2009, 30(3): 30-33.
雷冬至, 金曙光, 乌仁塔娜. 用体外产气法评价不同粗饲料与相同精料间的组合效应. 饲料工业, 2009, 30(3): 30-33.
[22] Xue H F, Meng Q X. Recent nutritional advances of neutral detergent fiber in dairy cow. Chinese Journal of Animal Nutrition, 2008, (1): 454-458.
薛红枫, 孟庆翔. 奶牛中性洗涤纤维营养研究进展. 动物营养学报, 2008, (1): 454-458.
[23] Hassanat F, Gervais R, Massé D, et al . Methane production, nutrient digestion, ruminal fermentation, N balance, and milk production of cows fed timothy silage- or alfalfa silage-based diets. Journal of Dairy Science, 2014, 97(10): 6463-6474.
[24] Depeters E J, Bath D L. Canola meal vensus cottonseed meal as the protein supplement in dairy diets. Journal of Dairy Science, 1986, 69: 148-154.
[25] Zhang J J. Study on Modelling of Forage Grading Index Parameters and Associative Effects in Mixed Forages[D]. Hohhot: Inner Mongolia Agriculture University, 2004.
张吉鹃. 粗饲料分级指数参数的模型变化及粗饲料科学搭配的组合效应研究[D]. 呼和浩特: 内蒙古农业大学, 2004.
[26] Hoover W H. Chemical factors involved in ruminal fiber digestion. Journal of Dairy Science, 1986, 69: 2755-2766.
[27] Ortega M E, Stern M D, Satte L D. The effect of rumen ammonia concentrate on dry matter disappearence in situ. Journal of Dairy Science, 1979, 62(Suppl. I): 76.
[28] Murphy J J, Kennelly J J. Effect of protein concentrate and protein source on the degradability of dry matter and protein in situ. Journal of Dairy Science, 1987, 70: 1841-1849.
[29] Han Z K, Chen J. The Rumen Digestion and Metabolism of Ruminant[M]. Beijing: Science Press, 1988: 16-80.
韩正康, 陈杰. 反刍动物瘤胃的消化和代谢[M]. 北京: 科学出版社, 1988: 16-80.
[30] Hu W L. The Research of Saponins on Rumen Fermentation and Methane Production and Animal Production Performance Impact[D]. Hangzhou: Zhejiang University, 2005.
胡伟莲. 皂甙对瘤胃发酵与甲烷产量及动物生产性能影响的研究[D]. 杭州: 浙江大学, 2005.
[31] Zhang S J, Chaudhry A S, Osman A. Associative effects of ensiling mixtures of sweet sorghum and alfalfa on nutritive value, fermentation and methane characteristics. Animal Feed Science and Technology, 2015, 206: 29-38.
[32] Li J G, An Y F. The Standardization Production Technology of Dairy Cattle[M]. Beijing: China Agriculture University Press, 2003: 105.
李建国, 安永福. 奶牛标准化生产技术[M]. 北京: 中国农业大学出版社, 2003: 105.
[33] Yu T F, Zang J J, Sun G Q. Associative effects of peanut vine and four kinds of roughages. Chinese Journal of Animal Nutrition, 2012, 24(7): 1246-1254.
于腾飞, 张杰杰, 孙国强. 花生蔓与4种粗饲料间组合效应的研究. 动物营养学报, 2012, 24(7): 1246-1254.

体外法评价玉米秸秆、谷草和玉米秸秆青贮饲料组合效应研究

Associative effects of cornstalk, millet straw, and corn stalk on silage digestibility in vitro

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