荒漠环境下10种沙拐枣的生理特征比较研究

doi:10.11686/cyxb2016334

摘要/Abstract

摘要： 目前,土地荒漠化形势严峻,严重威胁生态系统安全。而植物对防治土地荒漠化发挥重要的作用,因此筛选合适的物种进行荒漠恢复重建则显得极为重要。沙拐枣因其抗逆性强而成为新疆荒漠区主要的优势物种,其生理生态响应被广泛研究,但是系统、定量的比较不同种沙拐枣抗逆性的研究较为缺乏。为此本文以吐鲁番沙漠植物园中栽培的10种沙拐枣为试验材料,测定同一生境下不同种沙拐枣的7个抗逆生理指标,利用方差分析其种间差异,并结合主成分分析综合评价不同种沙拐枣的抗逆性。结果表明,不同种沙拐枣在同一生境下各生理指标表现出显著性差异(P<0.01);其中头状沙拐枣、乔木状沙拐枣、小沙拐枣的SOD、POD含量均表现较低水平,抗逆性较差;泡果沙拐枣以高脯氨酸含量表现良好抗逆性;小沙拐枣H₂O₂含量最高,不利于细胞正常代谢;综合分析抗逆性由强到弱为泡果沙拐枣>艾比湖沙拐枣>密刺沙拐枣>淡枝沙拐枣>奇台沙拐枣>心形沙拐枣>红果沙拐枣>乔木沙拐枣>头状沙拐枣>小沙拐枣,该结果与沙拐枣在新疆的生态分布极为吻合。试验数据可为荒漠植物沙拐枣的引种栽培和抗逆性筛选提供基础数据和科学依据。

Abstract: Desertification is a serious issue and especially so in Xinjiang, where it is a significant threat to ecosystem security. As plants play an important role in the prevention and control of desertification, screening suitable species for restoration projects is an important task. Calligonum is a dominant species in the desert region of Xinjiang and its resistance to drought and salt stress has been thoroughly researched. However, there are few quantitative analyses comparing the resistance of different Calligonum species. In this study, ten Calligonum species grown in the Turpan desert botanical garden were selected and their physiological characteristics analysed. Seven physiological indexes were measured. Species’ differences were examined using variance analysis, while their resistance was evaluated using principal component analysis. The results showed that the species’ physiological indexes in the same habitat showed significant differences (P<0.01). Superoxide dismutase (SOD) and peroxide enzyme (POD) contents were the lowest for C. caput-medsae, C. arborescens and C. pumilum, all of which showed poor resistance. Proline content was the highest for C. junceum, with strong resistance. H₂O₂ content was the highest for C. pumilum, which is not conducive for normal cell metabolism. The combined resistance analysis produced the following species’ order: C. junceum>C. ebi-nurcum>C. densum>C. leucocladum>C. klementzii>C. cordatum>C. rubicundum>C. arborescens>C. caput-medsae>C. pumilum. Calligonum species have different resistance mechanisms with different protective enzyme contents. These results are confirmed by the ecological distribution of Calligonum in Xinjiang. This experiment thus provides a scientific basis for screening and introducing Calligonum species to remedy desert conditions.

潘航, 冯缨, 王喜勇, 李岩. 荒漠环境下10种沙拐枣的生理特征比较研究[J]. 草业学报, 2017, 26(6): 68-75.

PAN Hang, FENG Ying, WANG Xi-Yong, LI Yan. Examination and comparison of the physiological characteristics of ten Calligonum species in a desert environment[J]. Acta Prataculturae Sinica, 2017, 26(6): 68-75.

参考文献

[1] Han L Y, Zhang Q, Yao Y B, et al . Characteristics and origins of drought disasters in Southwest China in nearly 60 years. Acta Geographica Sinica, 2014, 69(5): 632-639.
韩兰英, 张强, 姚玉璧, 等. 近60 年中国西南地区干旱灾害规律与成因. 地理学报, 2014, 69(5): 632-639.
[2] Zhang J Z, Zhang Q Y, Sun G F, et al . Effects of drought stress and re-watering on growth and photosynthesis of Hosta . Acta Prataculturae Sinica, 2014, 23(1): 167-176.
张金政, 张起源, 孙国峰, 等. 干旱胁迫及复水对玉簪生长和光合作用的影响. 草业学报, 2014, 23(1): 167-176.
[3] State Forestry Bureau. The fifth China desertification and desertification situation report[EB/OL]. http: //www.forestry.gov.cn/main/69/content-831684.html.
国家林业局. 第五次中国荒漠化和沙化状况报告[EB/OL]. http: //www.forestry.gov.cn/main/69/content-831684.html.
[4] Rathore V S, Singh J P, Bhardwaj S. Potential of native shrubs Haloxylon salicornicum and Calligonum polygonoides for restoration of degraded lands in arid western Rajasthan, India. Environmental Management, 2015, 55: 205-216.
[5] Mao Z M. Flora Xinjiangensis[M]. Urumqi: Xinjiang Science Technology Hygiene Publishing House, 1992: 265.
毛祖美. 新疆植物志[M]. 乌鲁木齐: 新疆科技卫生出版社, 1992: 265.
[6] Tao L, Ren J, Liu X M. Study on water-absorbing-model of two kinds of Calligonum seeds. Journal of Arid Land Resources and Environment, 2000, 14(3): 89-91.
陶玲, 任珺, 刘新民. 两种沙拐枣吸水率模型的研究. 干旱区资源与环境, 2000, 14(3): 89-91.
[7] Qiu Z J, Li Y, Zhong P F. Effects of PEG stress on physiological characteristics of Calligonum mongolicum in different geographical provenance. Acta Geographica Sinica, 2011, 20(3): 108-114.
邱真静, 李毅, 种培芳. PEG胁迫对不同地理种源沙拐枣生理特性的影响. 草业学报, 2011, 20(3): 108-114.
[8] Liu M Z, Liu X Z, Zhou Z Z, et al . Anatomical structures of young stems of Calligonum mongolicum and its ecological adaptabilities. Northern Horticulture, 2014, 16: 17-19.
刘明珍, 刘秀珍, 周忠泽, 等. 沙拐枣幼枝解剖结构及其生态适应性. 北方园艺, 2014, 16: 17-19.
[9] Yan H L, Zhang X M, Xu H, et al . Responses of Calligonum arborescens photosynthesis to water stress in Tarim highway shelterbelt. Journal of Desert Research, 2007, 27(3): 460-465.
闫海龙, 张希明, 许浩, 等. 塔里木沙漠公路防护林植物沙拐枣气体交换特性对干旱胁迫的响应. 中国沙漠, 2007, 27(3): 460-465.
[10] Yan H L, Zhang X M, Xu H, et al . Photosynthetic characteristics responses of three plants to drought stress in Tarim highway shelterbelt. Acta Ecologica Sinica, 2010, 30(10): 2519-2528.
闫海龙, 张希明, 许浩, 等. 塔里木沙漠公路防护林 3种植物光合特性对干旱胁迫的响应. 生态学报, 2010, 30(10): 2519-2528.
[11] Liu Z, Zeng F J, An G X, et al . Influence of irrigation amounts on seedling growth and biomass allocation of Calligonum caput-medusae at the southern fringe of the Taklimakan desert. Journal of Desert Research, 2012, 32(6): 1655-1661.
刘镇, 曾凡江, 安桂香, 等. 塔克拉玛干沙漠南缘头状沙拐枣幼苗生长和生物量分配对不同灌溉量的响应. 中国沙漠, 2012, 32(6): 1655-1661.
[12] Shan L S, Li Y, Zhang X M, et al . Research on water-consumption characteristics of Calligonum arborescens under different irrigation amounts. Journal of Natural Resources, 2012, 27(3): 440-449.
单立山, 李毅, 张希明, 等. 不同灌溉量条件下乔木状沙拐枣蒸腾耗水特性的研究. 自然资源学报, 2012, 27(3): 440-449.
[13] Huang C B, Zeng F J, Lei J Q. Comparison of drought resistance among three Calligonum in southern fringe of the Taklimakan desert. Acta Prataculturae Sinica, 2014, 23(3): 136-143.
黄彩变, 曾凡江, 雷加强. 塔克拉玛干沙漠南缘3个沙拐枣种的抗旱特性比较. 草业学报, 2014, 23(3): 136-143.
[14] Li H S. Principle and Technology of Plant Physiological and Biochemical Experiments[M]. Beijing: Higher Education Press, 2000.
李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[15] Wang Y, Chen J W, Di X Y. A comparative study on the SOD, POD, MDA and dissoluble protein of six provenances of Chinese pine ( Pinus tabulaeformis Carr.) under water stress. Ecology and Environmental Sciences, 2011, 20(10): 1449-1453.
王琰, 陈建文, 狄晓艳. 水分胁迫下不同油松种源SOD、POD、MDA及可溶性蛋白比较研究. 生态环境学报, 2011, 20(10): 1449-1453.
[16] Dou J H, Yu S X, Fan S L, et al . SOD and plant stress resistance. Molecular Plant Breeding, 2010, 8(2): 359-364.
窦俊辉, 喻树迅, 范术丽, 等. SOD与植物胁迫抗性. 分子植物育种, 2010, 8(2): 359-364.
[17] Gao F Y, Zhang J L, Liu Z P. Studies on effects of four species of color-leafed plants on SOD, POD activity of the cold-resistance in winter. Chinese Agricultural Science Bulletin, 2010, 26(5): 169-173.
高福元, 张吉立, 刘振平. 冬季低温对4种彩叶植物SOD、POD活性影响的研究. 中国农学通报, 2010, 26(5): 169-173.
[18] Qi J, Xu Z, Wang H Q, et al . Physiological and biochemical analysis of the leaves of Elymus under dry farming conditions. Acta Prataculturae Sinica, 2009, 18(1): 39-45.
祈娟, 徐柱, 王海清, 等. 旱作条件下披碱草属植物叶的生理生化特征分析. 草业学报, 2009, 18(1): 39-45.
[19] Zhao Y, Ma Y Q, Weng Y J. Variation of betaine and proline contents in wheat seedlings under salt stress. Journal of Plant Physiology and Molecular Biology, 2005, 31(1): 103-106.
赵勇, 马雅琴, 翁跃进. 盐胁迫下小麦甜菜碱和脯氨酸含量变化. 植物生理与分子生物学学报, 2005, 31(1): 103-106.
[20] Song X G, Yi X P. The generation and the role of hydrogen peroxide in plant. Journal of Lianyungang Teachers College, 2010, (4): 99-103.
宋喜贵, 佘小平. 植物体内过氧化氢的产生及其生理作用. 连云港师范高等专科学校学报, 2010, (4): 99-103.
[21] Zhang L X, Li S X. Research progress on relationships betaine and drought/salt resistance of plants. Acta Botanica Boreali-Occidentalia Sinica, 2004, 24(9): 1765-1771.
张立新, 李生秀. 甜菜碱与植物抗旱/盐性研究进展. 西北植物学报, 2004, 24(9): 1765-1771.
[22] Li X M, Sun B Y, Tan J Z. Research progress on application of glycine betanie in plant stress resistance. Journal of Agricultural Sciences, 2006, 27(3): 66-70.
李新梅, 孙丙耀, 谈建中. 甜菜碱与植物抗逆性关系的研究进展. 农业科学研究, 2006, 27(3): 66-70.
[23] Zhang Y H, He N P, Zhang G M, et al . Nitrogen deposition and Leymus chinensis leaf chlorophyll content in Inner Mongolian grassland. Acta Ecologica Sinica, 2013, 33(21): 6786-6794.
张云海, 何念鹏, 张光明, 等. 氮沉降强度和频率对羊草叶绿素含量的影响. 生态学报, 2013, 33(21): 6786-6794.
[24] Li X X, Liu B X, Guo Z T, et al . Effects of NaCl stress on photosynthesis characteristics and fast chlorophyll fluorescence induction dynamics of Pistacia chinensis leaves. Chinese Journal of Applied Ecology, 2013, 24(9): 2479-2484.
李旭新, 刘炳响, 郭智涛, 等. NaCl胁迫下黄连木叶片光合特性及快速叶绿素荧光诱导动力学曲线的变化. 应用生态学报, 2013, 24(9): 2479-2484.
[25] Pan X, Qiu Q, Li J Y, et al . Physiological indexes of six plant species from the Tibetan plateau under drought stress. Acta Ecologica Sinica, 2014, 34(13): 3558-3567.
潘昕, 邱权, 李吉跃, 等. 干旱胁迫对青藏高原6种植物生理指标的影响. 生态学报, 2014, 34(13): 3558-3567.
[26] Liu W X, Ma J Z. Study on proline accumulation patterns of six plant species under adversity stress. Pratacultural Science, 2010, 27(4): 134-138.
刘旻霞, 马建祖. 6种植物在逆境胁迫下脯氨酸的累积特点研究. 草业科学, 2010, 27(4): 134-138.
[27] Zhu H, Zu Y G, Wang W J, et al . Effect of proline on plant growth under different stress conditions. Journal of Northeast Forestry University, 2009, 37(4): 86-89.
朱虹, 祖元刚, 王文杰, 等. 逆境胁迫条件下脯氨酸对植物生长的影响. 东北林业大学学报, 2009, 37(4): 86-89.
[28] Li B, Jia X F, Bai Q W, et al . Effect of drought stress on proline accumulation of alfalfa. Bulletin of Botanical Research, 2003, 23(2): 189-191.
李波, 贾秀峰, 白庆武, 等. 干旱胁迫对苜蓿脯氨酸累积的影响. 植物研究, 2003, 23(2): 189-191.
[29] Sun H J, Wang S F, Chen Y T. Effects of salt stress on growth and physiological index of 6 tree species. Forest Research, 2009, 22(3): 315-324.
孙海菁, 王树凤, 陈益泰. 盐胁迫对6个树种的生长及生理指标的影响. 林业科学研究, 2009, 22(3): 315-324.
[30] Ai P F, Jin X J, Jin Z Z, et al . Evaluation of cold-resistant physiological indices of kernel apricot. Journal of Hebei University of Science and Technology, 2013, 34(1): 48-53, 59.
艾鹏飞, 金晓静, 靳占忠, 等. 仁用杏抗寒性生理指标评价的研究. 河北科技大学学报, 2013, 34(1): 48-53, 59.