不同收获期贯叶连翘花中抗氧化能力、主要活性物质变化及挥发性组分分离鉴定

doi:10.11686/cyxb2016484

草业学报 ›› 2017, Vol. 26 ›› Issue (9): 66-74.DOI: 10.11686/cyxb2016484

不同收获期贯叶连翘花中抗氧化能力、主要活性物质变化及挥发性组分分离鉴定

葛莉¹, 姚园园¹, 康天兰², 李京耀³, 何恒军⁴, 杨德龙¹, 栗孟飞^1,*

1.甘肃省干旱生境作物学重点实验室,甘肃农业大学生命科学技术学院,甘肃兰州730070;
2.甘肃省经济作物技术推广站,甘肃兰州730030;
3.甘肃省康县中药材技术指导站,甘肃陇南746500;
4.甘肃省康县恒茂源中药材专业合作社,甘肃陇南746500

收稿日期:2016-12-14 修回日期:2017-03-13 出版日期:2017-09-20 发布日期:2017-09-20
通讯作者: *通信作者Corresponding author. E-mail:lmf@gsau.edu.cn
作者简介:葛莉(1993-), 女, 甘肃宁县人,在读本科。E-mail:geli199308@163.com
基金资助:
国家自然科学基金(81560617,31360148), 甘肃省农业科技创新(GNCX-2016-12), 甘肃省中药材产业科技攻关(GYC14-03), 国家级大学生创新创业训练计划(201510733007)和甘肃农业大学SRTP(20150801)资助

Antioxidant capacity, bioactive compounds, and volatile constituents of flowers of Hypericum perforatum at different harvest stages

GE Li¹, YAO Yuan-Yuan¹, KANG Tian-Lan², LI Jing-Yao³, HE Heng-Jun⁴, YANG De-Long¹, LI Meng-Fei^1,*

1.Gansu Provincial Key Lab of Aridland Crop Science, College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2.Station of Industrial Crop Promotion of Gansu Province, Lanzhou 730030, China;
3.Guidance Station of Herbs Cultivation of Kangxian, Longnan 746500, China;
4.Heng Mao Yuan Professional Cooperative of Chinese Herbal Medicine, Longnan 746500, China

Received:2016-12-14 Revised:2017-03-13 Online:2017-09-20 Published:2017-09-20

摘要/Abstract

摘要： 为了探明不同收获期(花蕾期、盛花期、结果期)贯叶连翘花中抗氧化能力、总黄酮、酚类和金丝桃素含量等的变化,以栽培花器官为实验材料,采用HPLC和GC-MS等方法对70%乙醇提取液进行测定与分析。结果表明,花蕾期和盛花期贯叶连翘花提取液自由基抑制率(inhibition percentage, I%)和铁离子还原/氧化能力(ferric reducing/antioxidant power, FRAP)均显著高于结果期,花蕾期和盛花期二者之间无显著差异;总黄酮和酚类化合物以及金丝桃素含量均呈现为盛花期>花蕾期>结果期,且在P<0.05水平下达到显著差异;盛花期花中分离鉴定得到37种挥发性化学组分,其中,主要成分有1,1-二乙氧基-乙烷(19.26%)、1-十六醇(17.85%)、β-衣兰烯(10.71%)、(Z, Z)-9,12-十八碳二烯酰氯(8.42%)、十六烷酸乙酯(8.40%)、叶绿醇(5.79%)、石竹烯氧化物(4.56%)等。以上研究结果表明,花蕾期至盛花期采集贯叶连翘花器官较佳,提取液抗氧化能力较强,主要活性物质含量较高,挥发性成分较为丰富,该研究结果将对贯叶连翘生产、大面积种植栽培具有重要的参考价值和实践意义。

Abstract: The aim of this study was to investigate the antioxidant capacity and the contents of total flavonoids, phenolics, and hypericin in Hypericum perforatum flowers at different harvest stages (bud stage, blooming stage, fruit stage). Flowers at each stage were extracted in 70% ethanol, and the extracts were subjected to high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The I% (inhibition percentage) and FRAP (ferric reducing/antioxidant power) values were significantly higher at the bud and blooming stages than at the fruit stage, but did not differ significantly between the bud and blooming stages. The contents of total flavonoids, phenolics, and hypericin differed significantly among stages, with the highest contents at the blooming stage, followed by the bud stage, and then the fruit stage. Thirty-seven compounds were separated and identified in the 70% ethanol extracts from flowers at the blooming stage. The main components were 1,1-diethoxy-ethane (19.26%), 1-hexadecanol (17.85%), β-ylangene (10.71%), (Z,Z)-9,12-octadecadienoyl chloride (8.42%), hexadecanoic acid ethyl ester (8.40%), phytol (5.79%), and caryophyllene oxide (4.56%). These results indicated that the optimal harvest stage of H. perforatum flowers is at the bud to blooming stages, when the flowers contain high levels of bioactive compounds, antioxidants, and volatile components. This study provides important reference information for the large-scale cultivation of H. perforatum.

葛莉, 姚园园, 康天兰, 李京耀, 何恒军, 杨德龙, 栗孟飞. 不同收获期贯叶连翘花中抗氧化能力、主要活性物质变化及挥发性组分分离鉴定[J]. 草业学报, 2017, 26(9): 66-74.

GE Li, YAO Yuan-Yuan, KANG Tian-Lan, LI Jing-Yao, HE Heng-Jun, YANG De-Long, LI Meng-Fei. Antioxidant capacity, bioactive compounds, and volatile constituents of flowers of Hypericum perforatum at different harvest stages[J]. Acta Prataculturae Sinica, 2017, 26(9): 66-74.

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不同收获期贯叶连翘花中抗氧化能力、主要活性物质变化及挥发性组分分离鉴定

Antioxidant capacity, bioactive compounds, and volatile constituents of flowers of Hypericum perforatum at different harvest stages

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