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草业学报 ›› 2017, Vol. 26 ›› Issue (10): 87-98.DOI: 10.11686/cyxb2016502

• 研究论文 • 上一篇    下一篇

干旱胁迫对高羊茅航天诱变新品系生理特性的影响及综合评价

李娟, 雷霞, 王小利, 牟琼, 杨春燕, 吴佳海*   

  1. 贵州省农业科学院草业研究所,贵州 贵阳 550006
  • 收稿日期:2016-12-28 出版日期:2017-10-20 发布日期:2017-10-20
  • 通讯作者: 1848266168@qq.com
  • 作者简介:李娟(1982-),女,甘肃平凉人,副研究员,硕士。E-mail:xixingyue@126.com
  • 基金资助:
    贵州省联合基金(黔科合J字LKN[2013]24号),贵州省重大专项(黔科合重大专项字[2014]6017号)和贵州省高层次创新型人才培养(黔科合人才[2016]4024号)资助

Effects of drought stress on the physiological characteristics of new lines of Festuca arundinacea induced by spaceflight and their comprehensive evaluation

LI Juan, LEI Xia, WANG Xiao-Li, MOU Qiong, YANG Chun-Yan, WU Jia-Hai*   

  1. Prataculture Institute of Guizhou academy of Agricultural Sciences, Guiyang 550006, China
  • Received:2016-12-28 Online:2017-10-20 Published:2017-10-20

摘要: 为揭示高羊茅航天诱变新品系对干旱胁迫的响应和适应,培育出耐旱性强的新品种。以高羊茅航天诱变新品系12份及其亲本黔草1号高羊茅和贵州主推品种水城高羊茅为材料,采用盆栽试验,在人工气候室进行干旱胁迫处理,测定可溶性蛋白、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性、叶绿素(Chl)生理指标,并应用隶属函数法和灰色关联法对其生理指标及14份高羊茅材料耐旱性进行综合评价。结果表明,可溶性蛋白质随着干旱胁迫程度的增加总体呈上升趋势,SOD、POD、CAT含量随着干旱胁迫程度增加总体呈下降趋势,叶绿素含量随干旱胁迫程度加剧呈下降趋势。5个生理指标在14份材料间均存在显著性差异。利用隶属函数法对14份高羊茅材料进行综合耐旱性评价,SP5-32的平均隶属函数值最大,为0.533,耐旱性顺序为:SP5-32>SP5-75>水城高羊茅>SP5-60>SP5-94>SP5-97>SP5-89>SP5-71>SP5-7>SP5-85>SP5-42>SP5-5>黔草1号高羊茅>SP5-88。利用灰色关联性分析法得出各耐旱指标与耐旱性的关联序为:POD>可溶性蛋白质>SOD> CAT>Chl,关联度在0.55~0.65之间。因此,SP5-32更加适应干旱地区,干旱胁迫下保护酶活性大小、可溶性蛋白质含量与叶绿素含量对高羊茅材料的耐旱性是大致相同的。

Abstract: In this study, the ecophysiological characteristics of 14 lines of Festuca arundinacea, including some induced by spaceflight, were studied in a pot experiment. The plants were subjected to drought stress conditions, and physiological and biochemical indices including soluble protein content, chlorophyll (Chl) content, and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity were determined. These indexes of the 14 F. arundinacea were used to quantitatively evaluate their drought tolerance. The soluble protein content and Chl content increased, and the activities of SOD, POD, and CAT decreased under drought conditions. The drought resistance of the 14 F. arundinacea lines was calculated using the subordinate function, and the lines were ranked, from most drought tolerant to least drought tolerant, as follows: SP5-32>SP5-75>F. arundinacea cv. Shuicheng>SP5-60>SP5-94>SP5-97>SP5-89>SP5-71>SP5-7>SP5-85>SP5-42>SP5-5>F. arundinacea cv. Qiancao No.1>SP5-88. The average subordinate function of SP5-32, the most drought tolerant line, was 0.533. A grey correlative analysis was conducted to determine which indices were most strongly related to drought resistance. The indices were ranked, from strongest correlation with drought resistance to weakest, as follows: POD activity>soluble protein content>SOD activity>catalase activity>Chl content (correlation coefficients, 0.55-0.65). Therefore, SP5-32 is more suitable for cultivation in drought-affected areas as its antioxidant enzyme activity, soluble protein content, and chlorophyll content are unaffected by drought stress.