欢迎访问《草业学报》官方网站,今天是 分享到:

草业学报 ›› 2017, Vol. 26 ›› Issue (10): 129-139.DOI: 10.11686/cyxb2017134

• 研究论文 • 上一篇    下一篇

外源水杨酸对干旱胁迫下小冠花叶片活性氧水平及抗氧化系统的影响

马乐元, 陈年来*, 韩国君, 李良, 孙小妹   

  1. 甘肃农业大学资源与环境学院,甘肃 兰州 730070
  • 收稿日期:2017-03-21 出版日期:2017-10-20 发布日期:2017-10-20
  • 通讯作者: chennl@gsau.edu.cn
  • 作者简介:马乐元(1984-),男,甘肃武威人,在读博士。E-mail:119256800@gsau.edu.cn
  • 基金资助:
    甘肃农业大学盛彤笙科技创新基金“盐,旱胁迫及其交叉胁迫下牧草小冠花生长及渗透调节的研究”(GSAU-STS-1332)和甘肃省高等学校科研项目“根系分区交替灌溉作物的光合生理特性及节水效应研究”(2015A-076)资助

Protective effect of salicylic acid on reactive oxygen levels and antioxidant system of Coronilla varia seedlings under drought stress

MA Le-Yuan, CHEN Nian-Lai*, HAN Guo-Jun, LI Liang, SUN Xiao-Mei   

  1. College of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2017-03-21 Online:2017-10-20 Published:2017-10-20

摘要: 本研究以小冠花幼苗叶片为材料,用0.25,0.5,1和2 mmol/L外源水杨酸(SA)对植株进行叶面喷施,通过盆栽模拟干旱胁迫处理,测定幼苗叶片在连续干旱下膜脂过氧化指标、抗氧化酶活性和非酶抗氧化物含量,研究外源水杨酸对干旱胁迫下小冠花幼苗活性氧水平及抗氧化系统的影响。结果表明,0.5~2.0 mmol/L的水杨酸显著降低了干旱胁迫下小冠花叶片中超氧阴离子(O2)的产生速率、过氧化氢(H2O2)含量、丙二醛(MDA)含量及细胞膜透性,显著提高了过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性,提升了抗氧化指数,但对抗坏血酸(ASA)和谷胱甘肽(GSH)含量的影响不显著。在干旱胁迫第11天,1 mmol/L SA处理的小冠花叶片O2产生速率、MDA含量、细胞膜透性显著低于干旱处理79.78%,34.42%,36.96%(P<0.05);CAT酶活性显著高于干旱处理2.45倍(P<0.05);到干旱胁迫第 16天,SOD、POD酶活性比干旱处理提高了3.85和3.63倍。表明外源水杨酸能够降低干旱胁迫下小冠花叶片的活性氧水平,提高小冠花叶片抗氧化能力,缓解干旱胁迫造成的细胞膜脂过氧化损伤,提高了小冠花的抗旱性,尤其以1 mmol/L水杨酸效果最佳。

Abstract: The aim of these experiments was to determine whether exogenous salicylic acid (SA) could protect Coronilla varia seedlings against drought stress. Leaves of C. varia seedlings grown in pots were sprayed with salicylic acid at four concentrations (0.25, 0.5, 1 and 2 mmol/L) and then the membrane lipid peroxide index, antioxidant enzyme activity, and the contents of non-enzymatic antioxidants in seedling leaves were determined during a drought stress treatment. At concentrations of 0.5-2.0 mmol/L, SA decreased the superoxide anion (O2) production rate, hydrogen peroxide (H2O2) content, malondialdehyde (MDA) content, and cell membrane permeability, and increased the activities of the antioxidant enzymes catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), which increased the antioxidant index capacity. However, at concentrations of 0.5-2.0 mmol/L, SA did not affect the ascorbate acid (ASA) and glutathione (GSH) contents in seedling leaves. At 11 days of drought stress, the O2 production rate, MDA content, and cell membrane permeability were 79.78%, 34.42%, and 36.96% lower, respectively, and CAT activity was 2.45-fold higher in seedlings treated with 0.5 mmol/L SA than in untreated seedlings (P<0.05). At 16 days of drought stress, the SOD and POD activities were 3.85-fold and 3.63-fold higher, respectively, in seedlings treated with 0.5 mmol/L SA than in untreated seedlings. These results demonstrated that exogenous SA could improve the drought resistance of C. varia by reducing reactive oxygen levels under drought stress, increasing the activity of the antioxidant system, and alleviating oxidation damage to cell membranes resulting from drought-induced membrane lipid peroxidation. The most effective concentration of SA was 1 mmol/L.