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草业学报 ›› 2017, Vol. 26 ›› Issue (12): 160-169.DOI: 10.11686/cyxb2017071

• 研究论文 • 上一篇    下一篇

磷添加条件下摩西球囊霉与禾草内生真菌对多年生黑麦草生长的影响

郭艳娥, 王晓瑜, 高萍, 段廷玉*   

  1. 草地农业生态系统国家重点实验室,农业部草牧业创新重点实验室,兰州大学草地农业科技学院,甘肃 兰州 730020
  • 收稿日期:2017-03-01 修回日期:2017-04-17 出版日期:2017-12-20 发布日期:2017-12-20
  • 通讯作者: E-mail:duanty@lzu.edu.cn
  • 作者简介:郭艳娥(1991-),女,甘肃会宁人,在读硕士。E-mail:guoye15@lzu.edu.cn
  • 基金资助:
    国家绿肥产业技术体系(CARS-22),中央高校基本科研业务费(2022016zr0003)和教育部创新团队发展计划项目(IRT_17R50)资助

Effects of Glomus mosseae and grass endophytes on the growth of Lolium perenne under phosphorus addition

GUO Yan-E, WANG Xiao-Yu, GAO Ping, DUAN Ting-Yu*   

  1. State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
  • Received:2017-03-01 Revised:2017-04-17 Online:2017-12-20 Published:2017-12-20
  • Contact: E-mail:duanty@lzu.edu.cn

摘要: 本试验探究了不添加磷(P0)和添加50 mg/kg(P50)条件下,丛枝菌根(arbuscular mycorrhizae, AM)真菌摩西球囊霉(Glomus mosseae)和禾草内生真菌对多年生黑麦草生长、养分吸收和磷酸酶活性的影响。多年生黑麦草分别由含有禾草内生真菌(E+)和不含禾草内生真菌(E-)种子建植获得。结果表明:1)AM真菌与禾草内生真菌可显著影响多年生黑麦草的生物量(P<0.05),二者共同作用(AME+)时生物量最低。P0处理,以AM真菌E-植株生物量最高。P50条件下,对照(NME-)最高;2)P添加平均提高AM真菌的侵染率11.40%,P0和P50条件下E+植株较E-植株的菌根侵染率分别低18.65%和11.77%;3)AM真菌未显著影响P吸收(P<0.05)。禾草内生真菌增加了植株N含量,而AM真菌侵染降低了E+植株N含量;4)P添加显著提高了磷酸酶活性(P<0.05),不同处理以AME+的磷酸酶活性最高。两种共生微生物在不同P条件下,单独作用均有利于植物生长,共同作用时因对光合产物存在竞争,并未协同促进植物生长和养分吸收。

Abstract: This study aimed to determine the effects of arbuscular mycorrhizae fungi Glomus mosseae and grass endophytes on the growth, nutrient absorption and phosphatase activities of Lolium perenne without phosphorus addition (P0) and with 50 mg/kg phosphorus addition (P50). Seeds of perennial ryegrass infected with grass endophyte (E+) and without grass endophyte (E-) were used to establish plants with and without the endophytes. The results showed that: 1) AM fungi and grass endophytes had significant effects on the host biomass (P<0.05); plants infected by both AM fungi and grass endophytes (AME+) had the lowest biomass. Plants infected by only Glomus mosseae had the highest biomass under P0, while plants free of the two symbiosis fungi reached their highest biomass under P50. 2) Phosphorus addition increased mycorrhizal colonization of perennial ryegrass by 11.40%, while infection with grass endophytes inhibited plant mycorrhizal colonization by 18.65% and 11.77% under P0 and P50 respectively. 3) G. mosseae had no significant effect on the absorption of P (P<0.05). Grass endophytes increased the content of total N, while AM colonization decreased the total N of plants with grass endophytes. 4) Phosphorus addition significantly increased phosphatase activities (P<0.05). Plants infected by AM fungi and grass endophytes had the highest phosphatase activities across the treatments. Each of the two symbiosis fungi improved plant growth when taken alone; however, they showed competition on photosynthate and did not enhance plant growth and nutrition absorption when applied together.