Abstract: To determine the difference in salinity resistance of Zoysia species, Z. macrostachya, Z. metrella and Z. japonica were used to study the effects on leaf protective enzymes (POD, SOD, CAT), MDA, proline, chlorophyll, and soluble protein content under different salinity treatments (0, 0.20%, 0.40%, 0.60% and 0.80%) after a 40 days incubation. As the salt concentration increased, the changes in activities of leaf protective enzymes and the proline content increased. At 0.80% salt concentration, leaf protective enzymes of Z. macrostachya had higher activity and lower MDA and proline contents. At lower salt concentrations (<0.40%), as the salt concentration increased, the total chlorophyll content and the soluble protein content of all Zoysia species increased, while they decreased at higher concentrations (>0.60%). At 0.80% salt concentration, the total chlorophyll and soluble protein contents of Z. macrostachya were higher, and the decline was smaller than in the other Zoysia species. This showed that Z. macrostachya had the highest salt resistance of the three Zoysia species.