CoCl2对NaCl胁迫下大麦生长及幼苗生理指标的影响

doi:10.11686/cyxb20140318

草业学报 ›› 2014, Vol. 23 ›› Issue (3): 160-166.DOI: 10.11686/cyxb20140318

CoCl₂对NaCl胁迫下大麦生长及幼苗生理指标的影响

孟亚雄^1,2**,王世红^2,4**,汪军成¹,徐先良¹,赖勇¹,司二静¹,马小乐^1,2,李葆春^1,3,杨轲^1,2,王化俊^1,2,*

1.甘肃省作物遗传改良与种质创新重点实验室甘肃省干旱生境作物学重点实验室,甘肃兰州730070;
2.甘肃农业大学农学院,甘肃兰州 730070;
3.甘肃农业大学生命科学技术学院,甘肃兰州 730070;
4.甘肃省农业科学院小麦所,甘肃兰州 730070

收稿日期:2013-04-01 出版日期:2014-06-20 发布日期:2014-06-20
通讯作者: E-mail:whuajun@yahoo.com
作者简介:孟亚雄(1977-),男,甘肃会宁人,副教授,博士。E-mail:yxmeng1@163.com。王世红(1970-),男,甘肃宁县人,在读博士。E-mail:Wangshh0811@163.com。**共同第一作者。
基金资助:
国家大麦青稞产业技术体系(CR05),甘肃省自然基金(1208RJZA136)和甘肃省干旱生境作物学重点实验室开放基金(GSCS-2010-07)资助

Influences of CoCl₂ on the growth and seedling physiological indexes of Hordeum vulgare under NaCl stress

MENG Ya-xiong^1,2,WANG Shi-hong^2,4,WANG Jun-cheng¹,XU Xian-liang¹,LAI Yong¹,SI Er-jing¹,MA Xiao-le^1,2,LI Bao-chun^1,3,YANG Ke^1,2,WANG Hua-jun^1,2

1.Gansu Key Lab of Crop Improvement & Germplasm Enhancement Gansu provincial Key Lab of Aridland Crop Science, Lanzhou 730070, China;
2. College of Agronomy, Gansu Agriculture University, Lanzhou 730070, China;
3.College of Life Sciences and Technology, Gansu Agricultural University, Lanzhou 730070, China;
4. Institute of Wheat, Gansu Academy of Agricultural Sciences, Lanzhou 730070, china

Received:2013-04-01 Online:2014-06-20 Published:2014-06-20

摘要/Abstract

摘要： 为了明确钴对NaCl胁迫的缓解作用,本研究就CoCl₂对不同浓度NaCl胁迫下大麦种子的萌发、幼苗生长及内源激素与相关生理指标的影响进行了探讨。结果表明,随着NaCl胁迫程度的加重,大麦种子发芽势、发芽率显著降低,芽与根的生长受到显著抑制。幼苗脱落酸(ABA)与丙二醛(MDA)大量积累,抗氧化酶活性提高。加入0.1 mmol/L CoCl₂显著提高了NaCl胁迫下大麦种子的发芽势与发芽率,促进了芽与根的生长,抑制了幼苗ABA的积累,同时提高了抗氧化酶活性,降低了MDA的含量,且在较严重NaCl胁迫下作用更明显。CoCl₂提高了NaCl胁迫下幼苗(IAA+GA₃)/ABA的值,减缓了活性氧(ROS)的积累与膜脂过氧化进程,对种子萌发及幼苗生长起到了促进作用,在一定程度上提高了大麦幼苗对NaCl胁迫的适应能力。

Abstract: To determine the beneficial effect of cobalt on the growth of Hordeum vulgare under NaCl stress, the effects of addition of CoCl₂ on seed germination, seedling growth, endogenous hormones and some physiological indexes of barley seedlings under NaCl stress were studied. With the aggravation of NaCl stress, the germination vigor, germination capacity, shoot length and root length decreased considerably, while the contents of abscisic acid (ABA) and malondialdehyde (MDA) increased significantly, and the activities of antioxidant enzymes were enhanced. Under NaCl stress, germination vigor, germination capacity, shoot length and root length and the activities of antioxidant enzymes were elevated by the treatment of 0.1 mmol/L CoCl₂, compared with NaCl stress alone, and it dramatically inhibited the accumulation of MDA and ABA. This effect was more significant under the more serious NaCl stress. The results showed that the addition of CoCl₂ could improve (IAA+GA₃)/ABA, enhance the ability to clear up reactive oxygen species, reduce lipid peroxidation under NaCl stress, and promote seed germination, seedling growth and adaptive capacity of barley.

中图分类号:

S512.3

孟亚雄,王世红,汪军成,徐先良,赖勇,司二静,马小乐,李葆春,杨轲,王化俊. CoCl₂对NaCl胁迫下大麦生长及幼苗生理指标的影响[J]. 草业学报, 2014, 23(3): 160-166.

MENG Ya-xiong,WANG Shi-hong,WANG Jun-cheng,XU Xian-liang,LAI Yong,SI Er-jing,MA Xiao-le,LI Bao-chun,YANG Ke,WANG Hua-jun. Influences of CoCl₂ on the growth and seedling physiological indexes of Hordeum vulgare under NaCl stress[J]. Acta Prataculturae Sinica, 2014, 23(3): 160-166.

参考文献

Reference：
[1]Meloni D A, Gulotta M R, Martinez C A. Salinity tolerance in Schinopsis quebracho colorado: seed germination, growth, ion relations and metabolic responses[J]. Journal of Arid Environments, 2008, 72(10): 1785-1792.
[2]Ramezani S, Parsa M B, Naderi M. Effect of physical and chemical treatments on seed germination and dormancy breaking of Prosopis farcta[J]. Journal of Natural and Engineering Sciences, 2010, 4(1): 45-48.
[3]Liu B X, Wang Z G, Yang M S, et al. Effects of simulated salt stress on seed germination, seedling emergence and growth of Ulmus pumila[J]. Acta Prataculturae Sinica, 2012, 21(5): 39-46.
[4]Zhang Y F, Laing W Z, Sui L, et al. Effect on physiological characteristic of Medicago sativa under saline-alkali stress at seeding stage[J]. Acta Prataculturae Sinica, 2009, 18(4): 230-235.
[5]Sun L J. Genetic resources and superior germplasm of Chinese barley[M]. Beijing: China Agricultural Science and Technology Press, 2001.
[6]Banuelos G S, Ajwa H A. Trace elements in soils and plants: An overview[J]. Journal of Environmental Science and Health, 1999, 34(4): 951-974.
[7]Liu X H, Li J Y. Cobalt research[J]. Acta Pedologica Sinica, 1995, 32(1): 112-161.
[8]McDowell I R. Minerals in animal and human nutrition（2nd Edition）[M]. Amsterdam, Netherlands: Academic Press, 2003: 277-296.
[9]Jaleel C A, Zhao C X, Jayakumar K, et al. Low concentration of cobalt increases growth, biochemical constituents, mineral status and yield inZea mays[J]. Journal of Scientific Research, 2009, 1(1): 128-137.
[10]Liu S P, Fan W H. The effect of cobalt on growth of tomato[J]. Chinese Journal of Soil Science, 2005, 36(6): 925-928.
[11]Huang M F, He Z X, Cai S M, et al. Effects of cobalt and molybdenum fertilizer on growth, seed yields and quality of Centrosema pubescens in humid subtropical areas[J]. Acta Agrectir Sinica, 2012, 2(2): 312-317.
[12]Guo X, Jie X L, Li M, et al. Regulating effects of selenium and its combining application with cobalt on the growth of Medicago sativa L.[J]. Chinese Journal of Grassland, 2008, 30(6): 22-26.
[13]Jayakumar K, Jaleel C A, Zhao C X, et al. Cobalt induced variations in growth and pigment composition of Arachis hypogaea L[J]. Academic Journal of Plant Sciences, 2009, 2(2): 74-77.
[14]Ma J H, Yao J, Cohen D,et al. Ethylene inhibitors enhance in vitro root formation from apple shoot cultures[J]. Plant Cell Reports, 1998, 17(3): 211-214.
[15]Kpczyski J, Nemoykina A, Kepczyska E. Ethylene andin vitro rooting of rose shoots[J]. Plant Growth Regulation, 2006, 50: 23-28.
[16]Aery N C, Jagetiya B L. Effect of cobalt treatments on dry matter production of wheat and DTPA extractable cobalt content in soils[J]. Communications in Soil Science and Plant Analysis, 2000, 31: 1275-1286.
[17]Li Z Z. CoCl2对Na2COThe protective effects of cobalt on the plasma membrane of Medicago sativa seedling leaves under Na2CO3 stress[J]. Acta Prataculturae Sinica, 2007, 16(3): 49-54.
[18]Jiao J, Li Z Z, Haung G B. Protective effects and their mechanisms of cobalt on soybean seedling’s leaf under drought stress[J]. Chinese Journal of Applied Ecology, 2006, 17(5): 796-800.
[19]Hui Z L, Ma L, Li C Z. Influences of COCl2 on seed germination and seedling resistance of Lolium multifolorum under acid stress[J]. Pratacultural Science, 2012, 29(5): 753-758.
[20]Fan M H, Shi G, Ying Y, et al. Effect of CoCl2 on the growth and antioxidant physiological indexes of rape seedling under seawater stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2010, 30(12): 2459-2465.
[21]Lang S P, Lu R J. Comparative study on salt tolerance of barley cultivars (lines) at seed germination stage[J]. Acta Agriculturae Shanghai, 2008, 24(4): 83-87.
[22]Shi R Z, Xiao L T, Lin W H, et al. High performance liquid chromatographic determination of internal hormones in Inter-Subspecific Hybrid Rice[J]. Chinese Journal of Chromatography, 2002, 20(2): 148-150.
[23]Li H S. Experimental principles and techniques of plant physiology and biochemistry[M]. Beijing: Higher Education Press, 2006.
[24]Zou Q. Experimental guidance of plant physiology[M]. Beijing: China Agriculture Press, 2004.
[25]Tanaka K, Masuda R, Sugimoto T, et al. Water deficiency-induced changes in the contents of defensive substances against active oxygen in spinach leaves[J]. Agriculture and Biological Chemistry, 1990, 54(10): 2629-2634.
[26]Heath R L, Packer L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation[J]. Archives of Biochemistry and Biophsics, 1968, 125(1): 189-198.
[27]Xu Z H, Li J Y. Plant hormones research in China: past, present and future[J]. Bulletin of Botany, 2006, 23(5): 433-442.
[28]Hassanein R A, Hassanein A A, Haider A S, et al. Improving salt tolerance ofZea mays L. plants by presoaking their grains in glycine betaine[J]. Australian Journal of Basic and Applied Sciences, 2009, 3(2): 928-942.
[29]Hashem H A, Bassuony F M, Hassanein R A. Stigmasterol seed treatment alleviates the drastic effect of NaCl and improves quality and yield in flax plants[J]. Australian Journal of Crop Science, 2011, 5(13): 1858-1867.
[30]Grossmann K, Hansen H. Ethylene-triggered abscisic acid: a principle in plant growth regulation?[J]. Physiologia Plantarum, 2001, 113(1): 9-14.
[31]Locke J M, Bryce J H, Morris P C. Contrasting effects of ethylene perception and biosynthesis inhibitors on germination and seedling growth of barley (Hordeum vulgare L.)[J]. Journal of Experimental Botany, 2000, 51: 1843-1849.
[32]Siddikee M A, Chauhan P S, Sa T. Regulation of ethylene biosynthesis under salt stress in red pepper (Capsicum annuum L.) by 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing halotolerant bacteria[J]. Journal of Plant Growth Regulation, 2012, 31: 265-272.
[33]Kraft M, Kuglitsch R, Kwiatkowski J, et al. Indole-3-acetic acid and auxin herbicides up-regulate 9-cis-epoxycarotenoid dioxygenase gene expression and abscisic acid accumulation in cleavers (Galium aparine): interaction with ethylene[J]. Journal of Experimental Botany, 2007, 58(6): 1497-1503.
[34]Pan R P, Zhou Q, Zhou B, et al. Effects of salinization stress on growth and the antioxidant system of tall fescue[J]. Acta Prataculturae Sinica, 2012, 21(1): 112-117.
[35]Meloni D A, Oliva M A, Martinez C A, et al. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress[J]. Environmental and Experimental Botany, 2003, 49(1): 69-76.
[36]Baxter C J, Redestig H, Schauer N, et al. The metabolic response of heterotrophic arabidopsis cells to oxidative stress[J]. Plant Physiology, 2007, 143(1): 312-325.
[37]Farhoudi R, Hussain M, Lee D J. Modulation of enzymatic antioxidants improves the salinity resistance in canola (Brassica napus)[J]. International Journal of Agriculture & Biology, 2012, 14(3): 465-468.
[38]Pan Y, Wu L J, Yu Z L. Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch)[J]. Plant Growth Regulation, 2006, 49: 157-165.
[39]Yao S X, Chen S S, Xu D S, et al. Plant growth and responses of antioxidants of Chenopodium album to long term NaCl and KCl stress[J]. Plant Growth Regulation, 2010, 60: 115-125.
[40]Tewari R K, Kumar P, Sharma P N, et al. Modulation of oxidative stress responsive enzymes by excess cobalt[J]. Plant Science, 2002, 162(3): 381-388.
[41]Sinha P, Khurana N, Nautiyal N. Induction of oxidative stress and antioxidant enzymes by excess cobalt in mustard[J]. Journal of Plant Nutrition, 2012, 35(6): 952-960.
参考文献：
[1]Meloni D A, Gulotta M R, Martinez C A. Salinity tolerance in Schinopsis quebracho colorado: seed germination, growth, ion relations and metabolic responses[J]. Journal of Arid Environments, 2008, 72(10): 1785-1792.
[2]Ramezani S, Parsa M B, Naderi M. Effect of physical and chemical treatments on seed germination and dormancy breaking of Prosopis farcta[J]. Journal of Natural and Engineering Sciences, 2010, 4(1): 45-48.
[3]刘炳响, 王志刚, 杨敏生, 等. 模拟盐胁迫对白榆种子发芽、出苗及幼苗生长的影响[J]. 草业学报, 2012, 21(5): 39-46.
[4]张永锋, 梁正伟, 隋丽, 等. 盐碱胁迫对苗期紫花苜蓿生理特性的影响[J]. 草业学报, 2009, 18(4): 230-235.
[5]孙立军. 中国大麦遗传资源和优异种质[M]. 北京: 中国农业科技出版社, 2001.
[6]Banuelos G S, Ajwa H A. Trace elements in soils and plants: An overview[J]. Journal of Environmental Science and Health, 1999, 34(4): 951-974.
[7]刘雪华, 李继云. 微量元素钴研究[J]. 土壤学报, 1995, 32(1): 112-161.
[8]McDowell I R. Minerals in Animal and Human Nutrition（2nd Edition）[M]. Amsterdam, Netherlands: Academic Press, 2003: 277-296.
[9]Jaleel C A, Zhao C X, Jayakumar K,et al. Low concentration of cobalt increases growth, biochemical constituents, mineral status and yield in Zea mays[J]. Journal of Scientific Research, 2009, 1(1): 128-137.
[10]刘素萍, 樊文华. 钴对番茄生长发育影响的初步研究[J]. 土壤通报, 2005, 36(6): 925-928.
[11]黄梅芬, 和占星, 薛世明, 等. 钴钼微肥对亚热带湿热地区距瓣豆生长种子产量及品质的影响[J]. 草地学报, 2012, 2(2): 312-317.
[12]郭孝, 介晓磊, 李明, 等. 硒以及硒钴配合施用对紫花苜蓿生长的调控效应[J]. 中国草地学报, 2008, 30(6): 22-26.
[13]Jayakumar K, Jaleel C A, Zhao C X,et al. Cobalt induced variations in growth and pigment composition of Arachis hypogaea L[J]. Academic Journal of Plant Sciences, 2009, 2(2): 74-77.
[14]Ma J H, Yao J, Cohen D,et al. Ethylene inhibitors enhance in vitro root formation from apple shoot cultures[J]. Plant Cell Reports, 1998, 17(3): 211-214.
[15]Kpczyski J, Nemoykina A, Kepczyska E. Ethylene and in vitro rooting of rose shoots[J]. Plant Growth Regulation, 2006, 50: 23-28.
[16]Aery N C, Jagetiya B L. Effect of cobalt treatments on dry matter production of wheat and DTPA extractable cobalt content in soils[J]. Communications in Soil Science and Plant Analysis, 2000, 31: 1275-1286.
[17]李朝周. CoCl2对Na2CO3胁迫下苜蓿幼苗叶片细胞膜的保护作用[J]. 草业学报, 2007, 16(3): 49-54.
[18]焦健, 李朝周, 黄高宝. 钴对干旱胁迫下大豆幼苗叶片的保护作用及机理[J].应用生态学报, 2006, 17(5): 796-800.
[19]回振龙, 马兰, 李朝周. CoCl2对酸胁迫下多花黑麦草种子萌发及幼苗抗性的影响[J]. 草业科学, 2012, 29(5): 753-758.
[20]范美华, 石戈, 应英, 等. 海水胁迫下CoCl2对油菜生长和抗氧化生理指标的影响[J]. 西北植物学报, 2010, 30(12): 2459-2465.
[21]郎淑平, 陆瑞菊. 不同大麦品种发芽期的耐盐性比较研究[J]. 上海农业学报, 2008, 24(4): 83-87.
[22]王若仲, 萧浪涛, 蔺万煌, 等. 亚种间杂交稻内源激素的高效液相色谱测定法[J]. 色谱, 2002, 20(2): 148-150.
[23]李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2006.
[24]邹琦. 植物生理实验指导[M]. 北京: 中国农业出版社, 2004.
[25]Tanaka K, Masuda R, Sugimoto T,et al. Water deficiency-induced changes in the contents of defensive substances against active oxygen in spinach leaves[J]. Agriculture and Biological Chemistry, 1990, 54(10): 2629-2634.
[26]Heath R L, Packer L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation[J]. Archives of Biochemistry and Biophsics, 1968, 125(1): 189-198.
[27]许智宏, 李家洋. 中国植物激素研究: 过去、现在和未来[J]. 植物学通报, 2006, 23(5): 433-442.
[28]Hassanein R A, Hassanein A A, Haider A S,et al. Improving salt tolerance of Zea mays L. plants by presoaking their grains in glycine betaine[J]. Australian Journal of Basic and Applied Sciences, 2009, 3(2): 928-942.
[29]Hashem H A, Bassuony F M, Hassanein R A. Stigmasterol seed treatment alleviates the drastic effect of NaCl and improves quality and yield in flax plants[J]. Australian Journal of Crop Science, 2011, 5(13): 1858-1867.
[30]Grossmann K, Hansen H. Ethylene-triggered abscisic acid: a principle in plant growth regulation?[J]. Physiologia Plantarum, 2001, 113(1): 9-14.
[31]Locke J M, Bryce J H, Morris P C. Contrasting effects of ethylene perception and biosynthesis inhibitors on germination and seedling growth of barley (Hordeum vulgare L.)[J]. Journal of Experimental Botany, 2000, 51: 1843-1849.
[32]Siddikee M A, Chauhan P S, Sa T. Regulation of ethylene biosynthesis under salt stress in red pepper (Capsicum annuum L.) by 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing halotolerant bacteria[J]. Journal of Plant Growth Regulation, 2012, 31: 265-272.
[33]Kraft M, Kuglitsch R, Kwiatkowski J,et al. Indole-3-acetic acid and auxin herbicides up-regulate 9-cis-epoxycarotenoid dioxygenase gene expression and abscisic acid accumulation in cleavers (Galium aparine): interaction with ethylene[J]. Journal of Experimental Botany, 2007, 58(6): 1497-1503.
[34]樊瑞苹, 周琴, 周波, 等. 盐胁迫对高羊茅生长及抗氧化系统的影响[J]. 草业学报, 2012, 21(1): 112-117.
[35]Meloni D A, Oliva M A, Martinez C A,et al. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress[J]. Environmental and Experimental Botany, 2003, 49(1): 69-76.
[36]Baxter C J, Redestig H, Schauer N,et al. The metabolic response of heterotrophic arabidopsis cells to oxidative stress[J]. Plant Physiology, 2007, 143(1): 312-325.
[37]Farhoudi R, Hussain M, Lee D J. Modulation of enzymatic antioxidants improves the salinity resistance in canola (Brassica napus)[J]. International Journal of Agriculture & Biology, 2012, 14(3): 465-468.
[38]Pan Y, Wu L J, Yu Z L. Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch)[J]. Plant Growth Regulation, 2006, 49: 157-165.
[39]Yao S X, Chen S S, Xu D S,et al. Plant growth and responses of antioxidants of Chenopodium albumto long-term NaCl and KCl stress[J]. Plant Growth Regulation, 2010, 60: 115-125.
[40]Tewari R K, Kumar P, Sharma P N,et al. Modulation of oxidative stress responsive enzymes by excess cobalt[J]. Plant Science, 2002, 162(3): 381-388.
[41]Sinha P, Khurana N, Nautiyal N. Induction of oxidative stress and antioxidant enzymes by excess cobalt in mustard[J]. Journal of Plant Nutrition, 2012, 35(6): 952-960.

CoCl₂对NaCl胁迫下大麦生长及幼苗生理指标的影响

Influences of CoCl₂ on the growth and seedling physiological indexes of Hordeum vulgare under NaCl stress

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics