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草业学报 ›› 2014, Vol. 23 ›› Issue (3): 282-289.DOI: 10.11686/cyxb20140334

• 论文 • 上一篇    下一篇

高寒草地禾草内生细菌B-401的鉴定及生物防治潜力评价

畅涛,王涵琦,杨成德*,王颖,杨小利,薛莉,陈秀蓉,徐长林   

  1. 草业生态系统教育部重点实验室 甘肃农业大学草业学院 甘肃省草业工程实验室 中-美草地畜牧业可持续发展研究中心,甘肃 兰州730070
  • 收稿日期:2013-05-15 出版日期:2014-06-20 发布日期:2014-06-20
  • 通讯作者: E-mail:yangcd@gsau.edu.cn
  • 作者简介:畅涛(1986-), 男,甘肃白银人, 在读硕士。E-mail:changt1986@126.com
  • 基金资助:
    国家自然科学基金(No.31160122)和草业生态系统教育部重点实验室(甘肃农业大学)开放课题项目(No.CYzs-2011011)资助

Identification and evaluation of biological control potential of B-401 endophytic bacteria in grasses on alpine grasslands

CHANG Tao,WANG Han-qi,YANG Cheng-de,WANG Ying,YANG Xiao-li,XUE Li,CHEN Xiu-rong,XU Chang-lin   

  1. Key Laboratory of Grassland Ecosystem, Gansu Agricultural University, Ministry of Education, College of pratacultural Science, Gansu Agricultural University, Pratacultural Engineering Laboratory of Gansu Province; Sino-U.S. Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China
  • Received:2013-05-15 Online:2014-06-20 Published:2014-06-20

摘要: 为了明确高寒草地禾草内生细菌B-401的生物防治潜力,采用平板对峙法测定了其抑菌能力。抑菌谱表明,B-401对马铃薯坏疽病菌、马铃薯褐腐病菌、马铃薯炭疽病菌、马铃薯枯萎病菌、马铃薯干腐病菌、番茄早疫病菌、小麦根腐病菌、孜然根腐病菌和黄瓜枯萎病菌的抑制率分别达74.45%,71.57%,70.05%,48.01%,56.01%,60.25%,64.65%,45.01%和31.79%。在贮藏库中进行10倍液喷雾,对马铃薯坏疽病的防效达52.67%。生物学功能测定表明,该菌株在含有色氨酸和不含色氨酸的King培养基中分泌IAA分别为3.42和2.60 mg/L,且具有固氮能力,无溶磷能力。对B-401通过形态特征和生理生化测定,结合16S rDNA序列同源性分析,鉴定其为萎缩芽孢杆菌(Bacillus atrophaeus)。

Abstract: To study the biological control potential of B-401 endophytic bacteria in grasses on alpine grasslands, the determination of bacteriostatic ability was carried out by dual culture against plant pathogenic fungi. B-401 inhibited the growth of Phoma foveata, Stysanus stemonitis, Colletotrichum coccodes, Fusarium avenaceum, Fusarium oxysporum, Alternaria solani, Bipolaris sorokiniana and Fusarium solani by 74.45%, 71.57%, 70.05%, 48.01%, 56.01%, 60.25%, 64.65% and 45.01% respectively. The control efficacy of B-401 against potato gangrene was 52.67% when a 10 times dilution of fermentation broth was sprayed on potato tubers during the storage-period. The concentration of IAA secreted by B-401 in King’s medium containing 100 mg/L tryptophan was 3.42 mg/L, and the concentration in the medium without tryptophan was 2.60 mg/L. B-401 possessed the capacity for nitrogen fixation but it did not have the capacity for phosphate solubilization. Based on morphology and physiological and biochemical characters, combined with 16S rDNA gene sequence analysis, B-401 was indentified as Bacillus atrophaeus.

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