四种冷季型草坪草对二氧化硫胁迫的生理响应研究

doi:10.11686/cyxb20140429

摘要/Abstract

摘要： 本研究以4种冷季型草坪草高羊茅、多年生黑麦草、匍匐翦股颖、草地早熟禾为材料,采用人工模拟熏气法,以1.45,2.15,3.56和5.01 mg/m³ 4个SO₂浓度水平,测定4种草坪草生理生化指标,最终比较4种草坪草对SO₂的抗性。结果表明,4种草坪草叶绿素含量随着SO₂浓度升高而降低,丙二醛、可溶性糖和脯氨酸随着SO₂浓度升高而增加。而且SOD、POD、CAT活性都随着SO₂浓度升高呈现不同程度的增加。采用模糊数学隶属度对各项指标综合评定得出,4种草坪草对SO₂的抗性能力由强到弱顺序为高羊茅、多年生黑麦草、匍匐剪股颖、草地早熟禾。从这些结果我们可以推断在SO₂污染强的区域,适宜种植高羊茅和多年生黑麦草。

Abstract: The chlorophyll content,membrane lipid peroxidation,osmoregulation substances,and protective enzyme activity of four cold-season turfgrass species (Fescue arundinacea,Lolium perenne,Poa pratensis; Agrostis stolonifera) treated with SO₂ (1.45,2.15,3.56,or 5.01 mg/m³) were measured using artificial simulation,and the resistance to sulfur was evaluated. The MDA content increased to different extents in these four turfgrass species which indicated that the membrane systems were injured under the SO₂ stress. F. arundinacea and L. perenne had increased antioxidant enzyme activities (SOD,POD and CAT) and accumulated compatible solutes content (soluble sugar and proline) against low level SO₂ stress but high concentrations of SO₂ had a significant effect on them. Additionally,A. stolonifera and P. pratensis had a poor resistance to SO₂ toxicity that was directly reflected in the observed appearance,especially for P. pratensis. The damage increased with SO₂ concentration. The results of a comprehensive evaluation showed that the SO₂ resistance of the four species decreased in the order F. arundinacea,L. perenne,A. stolonifera,and P. pratensis. The turfgrass species F. arundinacea and L. perenne would be suitable for urban landscaping in SO₂ polluted areas.

中图分类号:

S543.034

孙凌霞，孙萍，蔡仕珍，李西. 四种冷季型草坪草对二氧化硫胁迫的生理响应研究[J]. 草业学报, 2014, 23(4): 237-244.

SUN Ling-xia,SUN Ping,CAI Shi-zhen,LI Xi. Physiological responses of four cold-season turfgrasses to oxidative stress of SO₂[J]. Acta Prataculturae Sinica, 2014, 23(4): 237-244.

参考文献

Reference：
[1]Huang J. Study on the relationship and influence mechanism of environmental pollution and economic sustainable development[D]. Changsha: Hunan University, 2010. 
[2]Bian H F, Liu J L. Air pollution and bio-control study in Chinese city[J]. Journal of Northeast Normal University, 2001, 33(1): 79-83. 
[3]Zhou Q. Pollution and countermeasures of prevention and control of SO2in the air[J]. Inner Mongolia Environmental Protection, 2002, 14(3): 12-14. 
[4]Xiao S, Zhang X Q. Review of the research and application of grass in the remediation of contaminated environment[J]. Journal of Anhui Agricultural Science, 2007, 35(36): 11961-11964. 
[5]Hao J J, Kang Z L. Plant Physiology experimental techniques[M]. Beijing: Chemical Industry Press, 2006. 
[6]Wang X K. Plant Physiology and Biochemistry test principles and techniques[M]. Beijing: Higher Education Press, 2006. 
[7]Zhang Z L. Plant Physiology experimental guidance (second edition)[M]. Beijing: Higher Education Press, 1990: 175-185. 
[8]Li H S. Plant Physiology and Biochemistry experimental gardens and measurement techniques[M]. Beijing: Higher Education Press, 2003: 164-167. 
[9]Song F M, Ge X C, Zheng Z. The roles of active oxygen species and lipid perosidation in the resistance of cotton seedlings to fusarium wilt[J]. Acta Phytopathologica Sinica, 2001, 31(2): 110-116. 
[10]Wu M J, Sun X J, Lei Q F, et al. Determination of Sulfur in tobacco with Barium Sulphate Turbidimetry[J]. Tobacco Science & Technology, 2005, (1): 24-26. 
[11]Alfani A, Baldantoni D, Maisto G. Temporal and spatial variation in C, N, S and trace element contents in the leaves of Quercus ilex within the urban area of Naples[J]. Environmental Pollution, 2000, 109(1): 119-129.
[12]Zhong P F, Su S P. Physiological responses of four golden-leaf trees to SO2 stress[J]. Acta Ecologica Sinica, 2013, 33(15): 4639-4648. 
[13]Xue X D, Dong X Y, Duan Y X, et al. A comparison of salt resistance of three kinds of Zoysia at different salt concentrations[J]. Acta Prataculturae Sinica, 2013, 22(6): 315-320. 
[14]Wang L H, Li X, Liu W, et al. A study on resistance and purifying ability of SO2 on four warm-season turfgrasses[J]. Acta Prataculturae Sinica, 2013, 22(1): 225-233.
[15]Yi H L, Liu J, Zheng K. Effect of sulfur dioxide hydrates exposure on cell cycle, sister chromatid exchange and micronuclei in barley seedlings[J]. Ecotoxicology and Environmental Safety, 2005, 62: 421-426. 
[16]Li W. Studies on absorption and resistance of sulfur dioxide under acute stress to three ornamental fruit trees[D]. Changchun: Jilin Agricultural University, 2008. 
[17]Deltoro V I, Gimeno C, Calatayud. An effect of SO2 fumigation on photosynthetic CO2 gas exchange, chlorophyll a fluorescence emission and antioxidant enzymes in the lichens Evernia prunastri and Ramalina farinacea[J]. Plant Physiology, 1999, 105: 648-654. 
[18]Qian Y C. Oxidation of sulfur dioxide on plants and anti-oxidation of plants[J]. Plant Physiology Communications, 1991, 27(5): 326-331. 
[19]Huang F. Effect of SO2 on crop damage response and physiological and biochemical changes[D]. Jinzhong: Shanxi Agricultural University, 2004. 
[20]Tian B Z. Study of six ornamental tree species on sulfur dioxide stress resistance[D]. Taian: Shandong Agricultural University, 2006.
[21]Li Y H, Li X Y, Bai R Q, et al. Resistance to sulfur dioxide of four colored-leaf species in prunus[J]. Scienta Silvae Sinicae, 2008, 44(2): 28-33. 
[22]Hu D M, Sun M G, Wang T M, et al. SOEffect of sulfur dioxide stress on membrane lipid peroxidation and protective enzymes of three prnamental tree seedlings[J]. Journal of Shandong Agricultural University(Natural Science), 2005, 36(2): 175-180. 
[23]Gao J X, Pang F Y, Zhou X B. Effect of sulfur dioxide on plant metabolism[J]. Research of Enbironmental Sciences, 1997, 11(6): 5-9. 
[24]Pang W, Zhang W Q, Gan X H, et al. Effects of SO2 stress on photosynthetic physiology of garden plant seedlings[J]. Science of Soil and Water Conservation, 2013, 22(4): 82-87. 
[25]Zhao X L. Effects of sulfur dioxide stress on the physiological and biochemical indexes of fifteen ornamental tree species[D]. Lanzhou: Gansu Agricultural University, 2008. 
[26]Yu L, Liu Y H, Zhou L P, et al. A study on the changes of ascorbic acid and related physiological indexes in different cultivars of Zoysia under drought stress[J]. Acta Prataculturae Sinica, 2013, 22(4): 106-115.
[27]Noji M, Saito M, Nakamura M, et al. Cysteine synthase overexpression in tobacco confers tolerance to sulfur containing environmental pollutants[J]. Plant Physiology, 2001, 126(3): 973-980.
[28]Darkó , Ambrus H, Fodor J, et al. Enhanced tolerance to oxidative stress with elevated antioxidant capacity in doubled haploid maize derived from microspores exposed to paraquat[J]. Crop Science, 2009, 49: 628-636. 
[29]Suro’wka, Karolewski P, Niewiadomska E, et al. Antioxidative response of Mesembryanthemum crystallinum plants to exogenous SO2 application[J]. Plant Science, 2007, 172: 76-84.
[30]Li L H, Yi H L. Effect of sulfur dioxide on ROS production, gene expression and antioxidant enzyme activity in Arabidopsis plants[J]. Plant Physiology Biochemistry, 2012, 58: 46-53. 
[31]Zhang J Y, Zhu N, Li H, et al. Difference analysis on foliage sulfur content of 10 common afforestation trees[J]. Journal of Northwest Normal University(Natural Science), 2012, (4): 115-120.
[32]Luo H Y, Li J Y, Liu Z. Effect of purifying SO2 in atmosphere by greening tree species[J]. Journal of Beijing Forstry University, 2000, (1): 45-50.

参考文献：
[1]黄菁. 环境污染与经济可持续发展的关系及影响机制研究[D]. 长沙: 湖南大学, 2010. 
[2]边红枫, 刘静玲. 我国城市大气污染及其生物防治对策研究[J]. 东北师范大学学报(自然科学版), 2001, 33(1): 79-83. 
[3]周琴. 大气中二氧化硫的污染及防治对策[J]. 内蒙古环境保护, 2002, 14(3): 12-14. 
[4]肖苏, 张新全. 草类植物在污染环境修复中的研究应用概述[J]. 安徽农业科学, 2007, 35(36): 11961-11964. 
[5]郝建军, 康宗利. 植物生理学实验技术[M]. 北京: 化学工业出版社, 2006. 
[6]王学奎. 植物生理生化试验原理和技术[M]. 北京: 高等教育出版社, 2006. 
[7]张志良. 植物生理学实验指导(第二版)[M]. 北京: 高等教育出版社, 1990: 175-185. 
[8]李合生. 植物生理生化实验园林和测定技术[M]. 北京: 高等教育出版社, 2003: 164-167. 
[9]宋凤鸣, 葛秀春, 郑重. 活性氧及膜脂过氧化与棉花对枯萎病抗性的关系[J]. 植物病理学报, 2001, 31(2): 110-116. 
[10]吴名剑, 孙贤军, 雷启福, 等. 硫酸钡溶胶比浊法测定烟草中的硫[J]. 烟草科技, 2005, (1): 24-26. 
[11]Alfani A, Baldantoni D, Maisto G. Temporal and spatial variation in C, N, S and trace element contents in the leaves of Quercus ilexwithin the urban area of Naples[J]. Environmental Pollution, 2000, 109(1): 119-129.
[12]种培芳, 苏世平. 4种金色叶树木对SO2胁迫的生理响应[J]. 生态学报, 2013, 33(15): 4639-4648. 
[13]薛秀栋, 董晓颖, 段艳欣, 等. 不同盐浓度下3种结缕草的耐盐性比较研究[J]. 草业学报, 2013, 22(6): 315-320. 
[14]王丽华, 李西, 刘尉, 等. ４种暖季型草坪草对SO2的抗性及净化能力的比较[J]. 草业学报, 2013, 22(1): 225-233.
[15]Yi H L, Liu J, Zheng K. Effect of sulfur dioxide hydrates exposure on cell cycle, sister chromatid exchange and micronuclei in barley seedlings[J]. Ecotoxicology and Environmental Safety, 2005, 62: 421-426. 
[16]李伟. 三种观赏果树在二氧化硫气体急性胁迫下的吸收特性及抗性能力的研究[D]. 长春:吉林农业大学, 2008. 
[17]Deltoro V I, Gimeno C, Calatayud. An effect of SO2 fumigation on photosynthetic CO2 gas exchange, chlorophyll a fluorescence emission and antioxidant enzymes in the lichens Evernia prunastri and Ramalina farinacea[J]. Plant Physiology, 1999, 105: 648-654. 
[18]钱永常. SO2对植物的氧化作用和植物的抗氧化作用[J]. 植物生理学通讯, 1991, 27(5): 326-331. 
[19]黄芳. SO2对作物的伤害反应及生理生化变化的影响[D]. 晋中: 山西农业大学, 2004. 
[20]田宝状. 6个园林绿化树种对SO2抗性的研究[D]. 泰安: 山东农业大学, 2006.
[21]李彦慧, 李向应, 白瑞琴, 等. 4种李属彩叶树木对SO2的抗性[J]. 林业科学, 2008, 44(2): 28-33. 
[22]胡丁猛, 孙明高, 王太明, 等. SO2对三种园林绿化苗木叶片膜脂过氧化和保护酶的影响[J]. 山东农业大学学报(自然科学版), 2005, 36(2): 175-180. 
[23]高吉喜, 潘凤云, 周兴宝. 二氧化硫对植物新陈代谢的影响[J]. 环境科学研究, 1997, 11(6): 5-9. 
[24]潘文, 张卫强, 甘先华, 等. SO2胁迫对园林植物幼苗光合生理特征的影响[J]. 中国水土保持科学, 2013, 22(4): 82-87. 
[25]赵晓丽. SO2胁迫对15种园林绿化树种生理生化指标的影响[D]. 兰州: 甘肃农业大学, 2008. 
[26]俞乐, 刘拥海, 周丽萍, 等. 干旱胁迫下结缕草叶片抗坏血酸与相关生理指标变化的品种差异研究[J]. 草业学报, 2013, 22(4): 106-115.
[27]Noji M, Saito M, Nakamura M, et al. Cysteine synthase overexpression in tobacco confers tolerance to sulfur-containing environmental pollutants[J]. Plant Physiology, 2001, 126(3): 973-980.
[28]Darkó , Ambrus H, Fodor J, et al. Enhanced tolerance to oxidative stress with elevated antioxidant capacity in doubled haploid maize derived from microspores exposed to paraquat[J]. Crop Science, 2009, 49: 628-636. 
[29]Suro’wka, Karolewski P, Niewiadomska E, et al. Antioxidative response of Mesembryanthemum crystallinum plants to exogenous SO2 application[J]. Plant Science, 2007, 172: 76-84.
[30]Li L H, Yi H L. Effect of sulfur dioxide on ROS production, gene expression and antioxidant enzyme activity in Arabidopsisplants[J]. Plant Physiology Biochemistry， 2012, 58: 46-53. 
[31]张家洋, 朱南, 李慧, 等. 10种常见绿化树种叶片含硫量差异性分析[J]. 西北师范大学学报(自然科学版), 2012, (4): 115-120.
[32]罗红艳, 李吉跃, 刘增. 绿化树种对大气SO2的净化作用[J]. 北京林业大学学报, 2000, (1): 45-50.