甘肃省高寒草原牧草孢囊线虫的鉴定

doi:10.11686/cyxb2014372

草业学报 ›› 2015, Vol. 24 ›› Issue (8): 174-180.DOI: 10.11686/cyxb2014372

甘肃省高寒草原牧草孢囊线虫的鉴定

李惠霞¹, 彭焕², 彭德良², 朱锐东¹, 徐鹏刚¹, 李建荣¹

1.甘肃农业大学草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州 730070;
2.中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京 100193

出版日期:2015-08-20 发布日期:2015-08-20
基金资助:
国家自然科学基金(31460463)和草业生态系统教育部重点实验室(甘肃农业大学)开放课题基金(CYZS-2011010)资助; 作者简介:李惠霞(1972-),女,甘肃天水人,副教授; E-mail:lihx@gsau; edu; cn

Identification of cyst nematode in alpine meadow steppe, Gansu

LI Hui-Xia¹, PENG Huan², PENG De-Liang², ZHU Rui-Dong¹, XU Peng-Gang¹, LI Jian-Rong¹

1.College of Prataculturae, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou 730070, China;
2.State Key Laboratory for Biology of Plant Diseases and Insect Pest, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Online:2015-08-20 Published:2015-08-20

摘要/Abstract

摘要： 2013年8月下旬,对甘肃天祝高寒草甸草原孢囊线虫发生情况进行调查,从嵩草和矮嵩草根部和根际土样中分离得到孢囊线虫。通过对孢囊和孢囊阴门锥的形态观察,初步鉴定为禾谷孢囊线虫(Heterodera avenae)。其特征为孢囊浅褐色,阔柠檬形,阴门锥双膜孔,未见阴门下桥,泡状突明显。利用通用引物AB28和TW81对其群体rDNA-ITS区进行PCR扩增和序列测定,得到ITS区序列长度为1045 bp。将所测得序列在Genbank和Blast上比对,结果显示,此禾谷孢囊线虫群体与H. avenae群体相似度达99%~100%。进一步证实寄生于甘肃天祝高寒草甸草原嵩草和矮嵩草的孢囊线虫均为H. avenae,这是首次报道莎草科嵩草和矮嵩草也是禾谷孢囊线虫的寄主。

Abstract: A survey of grass cyst nematodes in Tianzhu alpine meadow steppe, Gansu Province was conducted in August, 2013. Cyst nematodes were isolated from the roots and root rhizosphere of Kobresia sp. and Kobresia humilis. The nematodes were initially identified as Heterodera avenae based on cyst characteristics, particularly the cyst vulva cones. The cysts were light brown, large lenmon-shaped and contained vulva cones with bifenestrate and obvious bullae and without a vulva under-bridge. The 1045 bp sequence fragment of ITS region was amplified using PCR with general primers AB28 and TW81. The similarities between these groups and H. avena were 99%-100%. Molecular analysis confirmed that the cyst nematodes isolated from Kobresia sp. and Kobresia humilis growing in Tianzhu alpine meadow steppe were H. avenae. This is the first report identifying Kobresia sp. and Kobresia humilis as hosts of H. avenae.

李惠霞, 彭焕, 彭德良, 朱锐东, 徐鹏刚, 李建荣. 甘肃省高寒草原牧草孢囊线虫的鉴定[J]. 草业学报, 2015, 24(8): 174-180.

LI Hui-Xia, PENG Huan, PENG De-Liang, ZHU Rui-Dong, XU Peng-Gang, LI Jian-Rong. Identification of cyst nematode in alpine meadow steppe, Gansu[J]. Acta Prataculturae Sinica, 2015, 24(8): 174-180.

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甘肃省高寒草原牧草孢囊线虫的鉴定

Identification of cyst nematode in alpine meadow steppe, Gansu

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