4种暖季型草坪草币斑病病原菌鉴定及其生物学特性

doi:10.11686/cyxb20150116

草业学报 ›› 2015, Vol. 24 ›› Issue (1): 124-131.DOI: 10.11686/cyxb20150116

4种暖季型草坪草币斑病病原菌鉴定及其生物学特性

章武¹, 刘国道², 南志标^{1*, *}

1.兰州大学草地农业科技学院,草地农业生态系统国家重点实验室,甘肃兰州 730020;
2.中国热带农业科学院热带作物品种资源研究所,农业部热带作物种质资源利用重点开放实验室,海南儋州571737

收稿日期:2013-05-06 出版日期:2015-01-20 发布日期:2015-01-20
通讯作者: zhibiao@lzu.edu.cn
作者简介:章武(1987-),男,江西抚州人,在读博士。E-mail:ldzw1987@163.com
基金资助:
国家公益性行业农业科技专项(201303057)和国家973计划项目(2014CB138702)资助

Identification and characterisation of Sclerotinia homoeoparpa causing leaf blight in 4 warm-season turfgrass species

ZHANG Wu¹, LIU Guodao², NAN Zhibiao^{1, *}

1.College of Pastoral Agriculture Science and Technology;
State Key Laboratory of Grassland Agro-ecosystems, Lanzhou University, Lanzhou 730020, China;
2.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Tropical Crops Germplasm Resources Utilization, Ministry of Agriculture, Danzhou 571737, China

Received:2013-05-06 Online:2015-01-20 Published:2015-01-20

摘要/Abstract

摘要： 2011-2013年对海南省多个高尔夫球场及校园绿化草坪进行调查期间发现一种类似于草坪币斑病且可严重危害4种暖季型草坪草的草坪病害。根据科赫法则验证、形态学观察以及ITS序列分析的结果,确定引起海南省币斑病的病原菌为Sclerotinia homoeocarpa,这是我国首次报道S. homoeocarpa危害杂交狗牙根和地毯草。生物学特性研究表明: S. homoeoparpa生长的适宜温度为25~30℃,最适温度为28℃;适宜pH值为4~6,最适pH值为5;菌丝的致死温度为55℃水浴处理10 min;光照有利于菌丝生长。S. homoeoparpa能够利用多种碳氮源:碳源测试中,对可溶性淀粉利用率较高,对D-甘露醇和D-阿拉伯糖利用率较低;氮源测试中,病菌对硝酸铵、磷酸二氢铵的利用效果最佳,对脲利用效果最差。

Abstract: This paper reports on field disease surveys carried out at several golf courses and college lawns in Hainan Province from 2011 to 2013. A serious disease similar to Dollar Spot was observed which affected 4 common warm-season turfgrass species. Based on comprehensive analyses including Koch’s postulates tests, the identification of morphological characteristics and rDNA-ITS sequencing, the pathogen causing Dollar Spot disease in Hainan province was identified as Sclerotinia homoeocarpa. To our knowledge, this is the first report that S. homoeocarpa can cause Dollar Spot in Burmudagrass and Carpetgrass in China. Biological characteristics analysis showed that S. homoeocarpa grew well in conditions where the temperature ranged from 25-30℃ and pH from 5-7. The optimum growth conditions were 28℃ and pH 5. Hyphallost their viability in agar plugs that were incubated at 55℃ for 10 minutes. Light promoted mycelium growth and all the tested carbon and nitrogen sources were utilized by S. homoeocarpa. Soluble starch had higher usage efficiency for mycelium growth than the other carbon sources, whereas D-mannitol and D-arabinose had lower usage efficiency. Ammonium nitrate and ammonium dihydrogen phosphate had higher usage efficiency for mycelium growth than the other nitrogen sources, whereas carbamide had lower usage efficiency.

章武, 刘国道, 南志标. 4种暖季型草坪草币斑病病原菌鉴定及其生物学特性[J]. 草业学报, 2015, 24(1): 124-131.

ZHANG Wu, LIU Guodao, NAN Zhibiao. Identification and characterisation of Sclerotinia homoeoparpa causing leaf blight in 4 warm-season turfgrass species[J]. Acta Prataculturae Sinica, 2015, 24(1): 124-131.

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4种暖季型草坪草币斑病病原菌鉴定及其生物学特性

Identification and characterisation of Sclerotinia homoeoparpa causing leaf blight in 4 warm-season turfgrass species

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