吸胀冷害下外源NO对紫花苜蓿种子萌发及抗氧化性的影响

doi:10.11686/cyxb20150410

摘要/Abstract

摘要： 用浓度为0.1 mmol/L外源NO供体硝普钠(sodium nitroprusside,SNP)处理4℃吸胀冷害下紫花苜蓿种子,来探讨吸胀冷害下NO对紫花苜蓿种子萌发及氧化损伤的影响。试验设计4个处理,分别为A:对照组(CK)为蒸馏水;B:4℃吸胀冷害组(Chilling);C: SNP;D:Chilling+SNP,每个处理重复3次。结果表明:1)紫花苜蓿种子经SNP处理12 h转入25℃继续培养84 h后,Chilling+SNP组比Chilling组发芽率、活力指数、发芽指数提高了14.37%,34.17%,1.29%。2)4℃吸胀冷害12 h后,Chilling+SNP组同Chilling组相比超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性分别提高了149.26%,60.35%,过氧化氢(H₂O₂)含量及超氧阴离子(·O₂^-)产生速率则降低了10.11%,27.61%。3)SNP对4℃吸胀冷害12,24和48 h 的种子体内羟自由基(·OH)的清除均呈下降趋势,Chilling+SNP组的清除率均高出SNP组,Chilling+SNP组在冷害12 h时清除率达最大值97.5%。综上所述,施加外源NO能促进种子的萌发,提高抗氧化酶活性,降低了活性氧对种子造成的伤害。

Abstract: Seeds of Medicago sativa were used to study the effects of 0.1 mmol/L sodium nitroprusside (SNP), as a source of nitric oxide, on seed germination and antioxidant capacity under imbibitional chilling at 4℃. Four treatments: control, chilling, SNP and chilling+SNP were replicated three times. The results indicated that after imbibitional chilling for 12 h and germination at 25℃ for 84 h, the vigor and germination of M. sativa seeds were increased by SNP treatment. The activities of antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT) increased 149.3% to 60.4% by SNP treatment. The radical clearance rate tended to decline with SNP treatments and chilling for 12, 24 and 48 h. At 12 h chilling the radical clearance rate reached a maximum of 97.5%. Imposing exogenous nitric oxide can promote germination, enhance antioxidant enzyme activities and reduce the reactive oxygen damage in M. sativa seed.

赵萌,魏小红. 吸胀冷害下外源NO对紫花苜蓿种子萌发及抗氧化性的影响[J]. 草业学报, 2015, 24(4): 87-94.

ZHAO Meng, WEI Xiao-Hong. Effects of nitric oxide on Medicago sativa seed germination under imbibitional chilling[J]. Acta Prataculturae Sinica, 2015, 24(4): 87-94.

参考文献

Zheng G H, Liang Z, Lin J. Studies on the imbibitional chilling injury in seeds and osmo conditioning. Bulletin of The Chinese Academy of Sciences, 2001, 3: 182-187.
Tao Z Y, Zou Q. Imbibitional chilling injury and imbibition damage in seeds. Plant Physiology Communications, 2000, 36(4): 368-376.
Xu Y, Wei X H. Effects of exogenous nitric oxide on seed germination and seedling oxidative damage in Medicago sativa under NaCl stress. Acta Prataculturae Sinica, 2013, 22(5): 145-153.
Tao Y, Wang Z. The study on physiological adaptability to cold resistance of alfalfa. Pratacultural Science, 2009, 26(9): 151-155.
Li Y, Li J J, Wei X H. Responses of antioxidative capability in horsebean seedling to NO and H₂O₂ under Cd stress. Acta Prataculturae Sinica, 2009, 18(6): 186-191.
Su T, Long R J, Wei X H, et al. Protective effects of exogenous nitric oxide on oxidative damage in oat seedling leaves under NaCl stress. Acta Prataculturae Sinica, 2008, 17(5): 48-53.
Beligni M V, Lamattina L. Is nitric oxide toxic or protective.Trends Plant Science, 1999, 4(8): 229-300.
Beligni M V, Lamattina L. Nitric oxide protects against cellular damage produced by methylviologen herbicides in potato plants.Nitric Oxide, 1999, 3(3): 199-228.
Marcela S, Carlos G, Guillermo E, et al. Nitric oxide as key component in hormone-regulated processes. Plant Cell Reports, 2013, 32: 853-866.
Garcia-Mata, Lamattina L. Nitric oxide induces stomatal closure and enhances the adaptive plant response against drought stress.Plant Physiology, 2001, 126: 1196-1204.
Ruan H H, Shen W B, Xu L L, et al. Protective effects of exogenous nitric oxide on oxidative damage in wheat seedling leaves under NaCl stress. Chinese Science Bulletin, 2001, 46(23): 1993-1997.
Zhang Y Y, Liu J, Liu Y L. Nitric oxide alleviates growth inhibition of maize seedlings under NaCl stress. Journal of Plant Physiology and Molecular Biology, 2004, 30(4): 455-459.
Zhang H, Shen W B, Xu L L. Effects of nitric oxide on the germination of wheat seeds and its reactive oxygen species metabolisms under osmotic stress. Acta Botanica Sinica, 2003, 45(8): 901-905.
Jiang Y B, Yang Y R, Zheng Q H. Effects of exogenous nitric oxide on antioxidase and chlorophyll fluorescence of seedling of alfalfa under drought stress. Agricultural Research in the Arid Areas, 2008, 26(2): 65-68.
Chen Y P, Wang X M, Yang Z J, et al. Effects of nitric oxide on seed germination and physiological reaction of maize seedlings under low temperature stress. Journal of Agro-Environment Science, 2012, 31(2): 270-277.
Yang M S, Hu X B, Luo H H, et al. Effect of exogenous nitric oxide on chilling tolerance and seed germination of cotton seed during seed imbibition. Cotton Science, 2012, 24(3): 265-271.
Li Y Y, He J M. Effect of nitric oxide on tomato seeds resisting against chilling imbibition. Acta Botanica Boreali-Occidentalia Sinica, 2008, 28(4): 0709-0717.
Ma X L, Wei X H, Long R J, et al. Studies on mechanism of enhancing the chilling resistance of annual ryegrass by exogenous nitric oxide. Acta Ecologica Sinica, 2005, 25(6): 1269-1274.
Ruan H H, Shen W B, Xu L L, et al. Effects of exogenous no donor on glutathione-dependent antioxidative system in wheat seedling leaf under salt stress. Acta Agronomica Sinica, 2005, 31(9): 1144-1149.
Zou Q. Experimental Guide of Physiology of Plant. Beijing: China Agriculture Press, 2000: 166-170.
Zhai F F, Han L, Liu J X, et al. Assessing cold resistance of mutagenic strains of perennial ryegrass under artificial low-temperature stress. Acta Prataculturae Sinica, 2013, 22(6): 268-279.
Wang A G, Luo G H. Quantitative relation between the reaction of hydroxylamine and superoxide anion radieals in plants. Plant Physiology Communicationas, 1990, 6: 55-57.
Fu L, Peng W W. Research on the antioxidant activity of Red Pigment from purple eggplant peel. Chinese Agricultural Science Bulletin, 2012, 28(24): 283-287.
Lu Y M, Su C Q, Li H F. Effects of different salts stress on seed germination and seedling growth of Trifolium repens. Acta Prataculturae Sinica, 2013, 22(4): 123-129.
Gu W Y, Qian Z, Xue Y, et al. Effects of nitric oxide on seed germination and growth of chicory under NaCl stress. Jiangsu Agricultural Sciences, 2013, 41(3): 174-176.
Ge C, Zhao C H, Wang X J, et al. Advance of physiologyical study on no promoting seed germination. Seed, 2010, 29(11): 55-59.
Li C Z, Jiao J, Wang G X. The important roles of reactive oxygen species in the relationship between ethylene and polyamines in leaves of spring wheat seedings under root osmotic stress. Plant Science, 2004, 166: 303-315.
Shang Q M, Chen S F, Zhang Z G. Regulation of selenium on antioxidative enzymes activity in Pepper leaves under high temperature stress. Acta Horticulturae Sinica, 2005, 32(1): 35-38.
Liu X J, Liu X N. Effect of paclobutrazol and unicomazole on some physiology and biochemical and the resistance of Poa pratensis. Acta Prataculturae Sinica, 2006, 15(2): 48-53.
Liu J X, Wang J C, Jia H Y, et al. Effects of exogenous nitric oxide on seed germination and reactive oxygen species metabolism of seeding leaves in ryegress under osmotic stress. Agricultural Research in the Arid Areas, 2012, 30(6): 128-133.
Tu J, Shen W B, Lin Q G, et al. Elimination of the interference of nitric oxide donor SNP in determining SOD activity of wheat leaves with NBT photoreduction method. Plant Physiology Communications, 2003, 39(5): 483-485.
Clark D, Durner J, Navarre D A, et al. Nitric oxide inhibition of tobacco catalase and ascorbate peroxidase. Molecular Plant-Microbe Interactions, 2000, 13(20): 1380-1384.
Hertwig B, Streb P, Feierabend J. Light dependence of catalase synthesis and degradation in leaves and the influence of interfering stress conditions.Plant Physiology, 1992, 100(4): 1547-1553.
Alberto Gonzalez, Rodrigo A C, Bernardo M, et al. Cross talk among calcium, hydrogen peroxide, and nitric oxide and activation of gene expression involving Calmodulins and Calcium-dependent protein Kinases in Ulva compressa expoesd to copper excess. Plant Physiology, 2012, 158: 1451-1462.
郑光华, 梁峥, 林坚.种子吸胀冷害和渗透调控的研究.中国科学院院刊, 2001, 3: 182-187.
陶宗娅, 邹琦.种子的吸胀冷害和吸胀伤害.植物生理学通讯, 2000, 36(4): 368-376.
徐严, 魏小红.外源NO对NaCl胁迫下紫花苜蓿种子萌发及幼苗氧化损伤的影响.草业学报, 2013, 22(5): 145-153.
陶雅, 王柱.紫花苜蓿的抗寒生理适应性研究.草业科学, 2009, 26(9): 151-155.
李源, 李金娟, 魏小红.镉胁迫下蚕豆幼苗抗氧化能力对外源NO和H₂O₂的响应.草业学报, 2009, 18(6): 186-191.
苏桐, 龙瑞军, 魏小红, 等.外源NO对NaCl胁迫下燕麦幼苗氧化损伤的保护作用.草业学报, 2008, 17(5): 48-53.
阮海华, 沈文彪, 徐朗莱, 等.一氧化氮对盐胁迫下小麦叶片氧化损伤的保护效应.科学通报, 2001, 46(23): 1993-1997.
张艳艳, 刘俊, 刘友良.一氧化氮缓解盐胁迫对玉米生长的抑制作用.植物生理与分子生物学学报, 2004, 30(4): 455-459.
张华, 沈文彪, 徐朗莱.一氧化氮对渗透胁迫下小麦种子萌发及其活性氧代谢的影响.植物学报, 2003, 45(8): 901-905.
姜义宝, 杨玉荣, 郑秋红.外源一氧化氮对干旱胁迫下苜蓿幼苗抗氧化酶活性和叶绿素荧光特性的影响.干旱地区农业研究, 2008, 26(2): 65-68.
陈银萍, 王晓梅, 杨宗娟, 等. NO对低温胁迫下玉米种子萌发及幼苗生理特性的影响.农业环境科学学报, 2012, 31(2): 270-277.
杨美森, 虎晓兵, 罗宏海, 等.外源一氧化氮对棉花种子吸胀期间耐冷性和发芽能力的影响. 棉花学报, 2012, 24(3): 265-271.
李艳艳, 贺军民.一氧化氮对番茄种子抗吸胀冷害的影响.西北植物学报, 2008, 28(4): 0709-0717.
马向丽, 魏小红, 龙瑞军, 等.外源一氧化氮提高一年生黑麦草抗冷性机制.生态学报, 2005, 25(6): 1269-1274.
阮海华, 沈文彪, 徐朗莱, 等.外源一氧化氮供体对盐胁迫下小麦幼苗叶片谷胱甘肽抗氧化酶系统的影响.作物学报, 2005, 31(9): 1144-1149.
邹琦.植物生理学实验指导.北京: 中国农业出版社, 2000: 166-170.
翟飞飞, 韩蕾, 刘俊祥, 等.人工低温下多年生黑麦草诱变株系的抗寒性研究.草业学报, 2013, 22(6): 268-279.
王爱国, 罗光华.植物的超氧物自由基与羟胺反应的定量关系.植物生理学通讯, 1990, 6: 55-57.
付莉, 彭威威.紫茄皮红色素抗氧化活性的研究.中国农业通讯, 2012, 28(24): 283-287.
卢艳敏, 苏长青, 李会芬.不同盐胁迫对白三叶种子萌发及幼苗生长的影响.草业学报, 2013, 22(4): 123-129.
谷文英, 钱泽, 薛洋, 等.外源一氧化氮对盐胁迫下菊苣种子萌发和芽苗生长的影响.江苏农业科学, 2013, 41(3): 174-176.
葛超, 赵成昊, 王孝杰, 等.一氧化氮促进种子萌发的生理研究进展.种子, 2010, 29(11): 55-59.
尚庆茂, 陈淑芳, 张志刚.硒对高温胁迫下辣椒叶片抗氧化酶活性的调节作用.园艺学报, 2005, 32(1): 35-38.
刘晓静, 柳小妮.多效唑和烯效唑对草地早熟禾一些生化指标及抗性的影响.草业学报, 2006, 15(2): 48-53.
刘建新, 王金成, 贾海燕, 等.外源一氧化氮对渗透胁迫下黑麦草种子萌发及活性氧代谢的影响.干旱地区农业研究, 2012, 30(6): 128-133.
屠洁, 沈文彪, 林国庆.等.一氧化氮供体SNP干扰NBT光化还原法测定小麦叶片SOD活性的清除.植物生理学通讯, 2003, 39(5): 483-485.