干旱及复水对山西野生草地早熟禾幼苗生长与生理特性的影响

doi:10.11686/cyxb2015098

摘要/Abstract

摘要： 以15份山西野生草地早熟禾种质为材料,待其长至4~5片真叶时,采用盆栽控水法进行21 d干旱及7 d复水处理,取样测定植株存活率、相对生长率(RGR)、叶片相对含水量(RWC)、游离脯氨酸(Pro)、超氧化物歧化酶(SOD)和丙二醛(MDA)含量,并根据隶属函数法对其抗旱性进行综合评价。结果表明,在干旱胁迫下,不同居群野生草地早熟禾幼苗的存活率、RGR、RWC和SOD活性下降,Pro、MDA含量则上升。复水后,各指标均有所恢复,但未能恢复至胁迫前水平。抗旱性综合分析将参试草地早熟禾分为3个类群,其中沁水Ⅱ、浑源Ⅰ、沁水Ⅰ、五台、浑源Ⅱ、应县、广灵和灵丘8个居群为Ⅱ级中等抗旱,垣曲、宁武、霍州、忻州、五寨和灵石6个居群为Ⅲ级弱抗旱,屯留居群为Ⅳ级不抗旱居群。沁水Ⅱ居群的隶属值最大,可作为重点对象进行选育研究。

Abstract: Research has been undertaken on the effects of drought and rewatering on the growth and physiological characteristics of 15 species wild Poa pratensis seedlings in Shanxi. Using pot tests, at the four-leaf stage the P. pratensis species were water-controlled for 21 days and then re-watered. Seedling survival rates, relative growth rates (RGR), relative water content (RWC), proline (Pro), and the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) content were measured on the 21st day after water-controlling and the 7th day after rewatering. All indexes were evaluated with the subordination function method to obtain the membership value of each population. Results showed that seedling survival rates, RGR, RWC and SOD declined during the 21-day drought stress. However, Pro and MDA contents increased. After rewatering, all the indexes rehabilitated to some degree but did not recover to the same level as the control groups. Data analysis showed the tested P. pratensis species could be grouped into three kinds. Class Ⅱ were medium drought-resistant populations, including QinshuiⅡ, HunyuanⅠ, QinshuiⅠ, Wutai, HunyuanⅡ, Yingxian, Guangling and Lingqiu. Class Ⅲ showed weaker drought resistance, including the Yuanqu, Ningwu, Huozhou, Xinzhou, Wuzhai and Lingshi populations. The Tunliu population was categorized as class Ⅳ, which had no drought resistance. The membership value of the QinshuiⅡpopulation was the highest, and could be used as the key selection object in the future.

朱慧森, 王保平, 董晓燕, 董宽虎, 李存福. 干旱及复水对山西野生草地早熟禾幼苗生长与生理特性的影响[J]. 草业学报, 2015, 24(7): 79-88.

ZHU Hui-Sen, WANG Bao-Ping, DONG Xiao-Yan, DONG Kuan-Hu, LI Cun-Fu. Effects of drought stress and rewatering on the growth and physiological characteristics of Shanxi wild Poa pratensis seedlings[J]. Acta Prataculturae Sinica, 2015, 24(7): 79-88.

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