立枯丝核菌病原致病性及苜蓿品种抗病性研究

doi:10.11686/cyxb2015197

摘要/Abstract

摘要： 为了探明立枯丝核菌对苜蓿种苗的致病性,本研究采用菌饼上放置种子的方法,将分离自3个地点6个苜蓿品种上的6个菌株接种于14个紫花苜蓿品种的种子上,14 d测定相对发芽率、根长、苗长和病情指数。结果表明,参试菌株的菌降低了种子的发芽,从金皇后分离到的菌株对苗长的抑制作用最强;致病性差异显著(P<0.05),其中从金皇后分离出菌株的致病性最强,从丰宝中分离出菌株的致病性最弱;从金皇后、8920MF和爱菲尼特中分离出的立枯丝核菌对苜蓿种子与幼苗的致病力较强;参试的苜蓿品种对同一菌株的抗病性不同,佛拿尔、阿尔冈金和苜蓿王的抗病力最强,丰宝、丰叶721抵抗力最弱。

Abstract: The aim of this study was to evaluate the pathogenicity of Rhizoctonia solani isolates to various alfalfa (Medicago sativa) varieties. We used 14 varieties of alfalfa and six pathogen isolates that were isolated from roots of six different alfalfa varieties from three locations. We estimated the disease index, relative germination rate, relative root length, and relative seedling length at 14 days after inoculation with the various isolates. There were significant differences among the 14 alfalfa varieties inoculated with the six pathogen isolates (P<0.05). The isolate from ‘Goldenqueen’ showed significantly stronger pathogenicity (P<0.05) than the other isolates. The isolate from ‘Powerplant’ showed the weakest pathogenicity. All the fungi used in this study resulted in lower germination rates and shorter roots of alfalfa seedlings, and the isolate from ‘Goldenqueen’ showed the strongest effects. Based on the comprehensive evaluation of all traits, R. solani isolated from ‘Goldenqueen’, ‘8920MF’, and ‘Affinity’ showed the strongest pathogenicity to alfalfa seedlings. Among the 14 alfalfa varieties, ‘Vernal’, ‘Algonguin’, and ‘Alfaking’ were more resistant to R. solani, while ‘Powerplant’ and ‘Amerileaf 721’ were the most sensitive.

郭玉霞, 张明, 管永卓, 刘芳, 郭志鹏, 张红瑞, 严学兵, 王成章. 立枯丝核菌病原致病性及苜蓿品种抗病性研究[J]. 草业学报, 2015, 24(11): 72-81.

GUO Yu-Xia, ZHANG Ming, GUAN Yong-Zhuo, LIU-Fang, GUO Zhi-Peng, ZHANG Hong-Rui, YAN Xue-Bing, WANG Cheng-Zhang. Pathogenicity of Rhizoctonia solani to alfalfa seedlings and disease resistance of alfalfa varieties[J]. Acta Prataculturae Sinica, 2015, 24(11): 72-81.

参考文献

[1] Yue Y H, Qi X, Wang Y R, et al . Persistence of 35 Medicago sativa varieties at the 10th year after establishment. Acta Prataculturae Sinica, 2014, 23(1): 58-64.
[2] Zhao M R, Shen Y H, Li Y C, et al . Research progress in the genetic engineering of alfalfa stress resistance. Acta Agrestia Sinica, 2014, 22(2): 243-248.
[3] Qi Z Q, Yu Y X, Hu Y G, et al . Current status and future tasks of the Medicago sativa industry in China. Acta Prataculturae Sinica, 2008, 17(1): 107-113.
[4] Guo Y X, Nan Z B, Wang C Z, et al . Progress in research on root invading fungi of Medicago sativa . Acta Prataculturae Sinica, 2009, 18(5): 243-249.
[5] Nan Z B. Root rot disease of Legume forage. Foreign Animal Husbandry-Grassland and Forage, 1991, (2): 5-11.
[6] Nan Z B, Li C J. Compendium of forage fungi disease in China. Pratacultural Science, 1994, (supplementary issue): 1-160.
[7] Chen Y, Min J C, Xiao F, et al . The preliminary study on root rot diseases of alfalfa in Xinjiang. Grassland of China, 1989, (2): 71-73.
[8] Nan Z B. Alfalfa diseases and its integrated control system in China. Animal Science and Veterinary Medicine, 2001, 18(4): M1-M4.
[9] Yuan Q H. Advances in alfalfa diseases in China. Plant Protection, 2007, 33(1): 6-12.
[10] Anderson N A. The genetics and pathology of Rhizoctonia solani . Annual Review of Phytopathology, 1982, (20): 329-347.
[11] Bita N. The effect of Rhizoctonia root rot on bean yield. Proceedings of the 20th Iranian Plant Protection Congress[C]. Iran: University of Shiraz, 2012.
[12] Wei L, Li X Y, Tang X F, et al . Screening on soybean germplasm resistant to Rhizoctonia solani . Soybean Science, 2012, 31(3): 457-461.
[13] Bita N. Epidemics of Rhizoctonia root rot in association with biological and physicochemical properties of field soil in bean crops. Journal of Phytopathology, 2013, 161(6): 397-404.
[14] Zhang Z Y, Leng H Q, Zhang Z M, et al . Science of Plant Pathogenic Fungi[M]. Chengdu: Sichuan Science and Technology Press, 1998: 347-348.
[15] Peng S Q, Zeng Z R, Zhang Z G. Sheath Blight ( Rhizoctonia solani ) of Rice and Its Control[M]. Shanghai: Shanghai Science and Technology Press, 1986: 46-79.
[16] Deng X M. Studies on the strains of pathogens of sheath blight of rice in Northwest Sichuan province. Journal of Southwest Agricultural University, 1991, 13(4): 380-383.
[17] Leah T. Biology, epidemiology and management of Rhizoctonia solani on potato. Journal of Phytopathology, 2010, 158(10): 649-658.
[18] Liu A P, Hou T J. The Diseases and Pests and Their Control of Grassland[M]. Beijing: China Agricultural Science and Technology Press, 2005: 28.
[19] Anderson J P, Bentley S, Irwin J A G, et al . Characterisation of Rhizoctonia solani isolates causing root canker of lucerne in Australia. Australasian Plant Pathology, 2004, 33(2): 241-247.
[20] Stuteville D L, Erwin D C. Compendium of Alfalfa Diseases[M]. Minnesota: The American Phytopathological Society Press, 1990: 1-59.
[21] Chen Y J, Cui G W. Investigations and pathogen isolation of root rot of alfalfa in Heilongjiang Province. Grassland of China, 2001, 23(3): 78-79.
[22] Guo Y X, Nan Z B, Li C J, et al . Root-invading fungi of lucerne and winter wheat in the rotation system on the Loess Plateau. Acta Ecologica Sinica, 2004, 24(3): 486-494.
[23] Nan Z B. Fungicide seed treatments of sainfoin control seed-borne and root-invading fungi. New Zealand Journal of Agricultural Research,1995, 38(3): 413-420.
[24] Li C J, Nan Z B. Seed-borne fungi of lucerne and their pathogenicity to lucerne seed and seedling. Acta Prataculturae Sinica, 2000, 9(1): 27-36.
[25] Galindo J J, Abaw I G S, Thurston H D. Variability among isolates of Rhizoctonia solani associated with snap been hypocotyls and soils in New York. Plant Disease, 1982, 66(5): 390-394.
[26] Hancock J G. Fungal rootlet colonization and forage yields of alfalfa in fungicide-treated field plots. Plant Disease, 1993, 77(6): 601-608.
[27] Li M Q, Chai Z X, Li J H, et al . Identification pathogens of crown and root rot of alfalfa in Dingxi region. Acta Agrestia Sinica, 2003, 21(1): 83-86.
[28] Aguirre R J, Hine R B, Schonhorst M H. Distribution of phytophthora root rot of alfalfa in central Mexico and development of disease resistance in Mexican cultivars of alfalfa. Plant Disease, 1983, 67(1): 91-93.
[29] Zhang J C, Xie Y J, Wei S X, et al . Research on the pathogenicity of Rhizoctonia solani on banana seedlings. Journal of Guangxi Agricultural and Biological Science, 2000, 19(2): 85-88.
[30] Yang J H, Bai L Y, Guo Q Y, et al . Anastomosis groups and their pathogenicity of Rhizoctonia solani Kǜhn isolated from Phaseolas vulgaris in Xinjiang. Xinjiang Agricultural Sciences, 2006, 43(4): 302-305.
[31] Wang Y L, Ren Y C, Zhang Z, et al . Characterization of glyceraldehyde-3-phosphate dehydrogenase gene sequences of different anastomosis group Rhizoctonia solani . China Journal of Rice Science, 2013, 27(6): 639-646.
[32] Kang X W, Long X B, Peng S Q, et al . Preliminary study on toxin of Rhizoctonia solani in rice sheath blight. Journal of Shenyang Agricultural University, 1992, 23(1): 19-22.
[33] Michaud R, Richard C. Evaluation of alfalfa cultivars for reaction to crown and root-rot. Canadian Journal of Plant Science, 1985,65(1): 95-98.
[34] Chen S K, Yao G J. Study of resistance to Pseudopeziza medicagines (Clib) Sacc on alfalfa cultivars. Pratacultural Science, 1994, 11(3): 61-62.
[35] Li M Q. Comparative pathogenicity of isolates of Fusarium spp. and cultivars resistance in alfalfa. Grassland of China, 2003, 25(1):39-43.
[36] Nyvall R F. Field Crop Diseases Handbook (2nd edition)[M]. New York: Kluwer Academic Publishers, 1989: 3-36.
[37] Alva A K, Lanyon L E, Leath K T. Influence of fungal-soil water interactions on phytophthora root rot of alfalfa. Biology and Fertility of Soils, 1985,1(2): 91-96.
[38] Summers C G. Integrated pest management in forage alfalfa. Integrated Pest Management Reviews, 1998, 3(3): 127-154.
[39] Harsharn S G. Zinc influences nodulation, disease severity, leaf drop and herbage yield of alfalfa cultivars. Plant and Soil, 2001, 234(1): 47-59.
[40] Hwang S F, Gossen B D, Turnbull G D, et al . Seedbed preparation, timing of seeding, fertility and root pathogens affect establishment and yield of alfalfa. Canadian Journal of Plant Science, 2002, 82: 371-381.
[41] Wiggins B E, Kinkel L L. Green manures and crop sequences influence alfalfa root rot and pathogen inhibitory activity among soil-borne streptomycetes. Plant and Soil, 2005, 268(1-2): 271-283.
[42] Han H W, Sun L N, Yao T, et al . Effects of bio-fertilizers with different PGPR strain combinations on yield and quality of alfalfa. Acta Prataculturae Sinica, 2013, 22(5): 104-112.
[43] Anderson J P, Lichtenzveig J, Oliver R P, et al . Medicago truncatula as a model host for studying legume infecting Rhizoctonia solani and identification of a locus affecting resistance to root canker. Plant Pathology, 2013, 62(4): 908-921.
[44] Chen D M, Chen X M, Liang Y J, et al . Influence of cropping system on enzyme activities and fungal communities in soil. Acta Prataculturae Sinica, 2015, 24(2): 77-84.
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[2] 赵美荣, 申玉华, 李永春, 等. 紫花苜蓿抗逆基因工程研究进展. 草地学报, 2014, 22(2): 243-248.
[3] 戚志强, 玉永雄, 胡跃高, 等. 当前我国苜蓿产业发展的形势与任务. 草业学报, 2008, 17(1): 107-113.
[4] 郭玉霞, 南志标, 王成章, 等. 苜蓿根部入侵真菌研究进展. 草业学报, 2009, 18(5): 243-249.
[5] 南志标. 豆科牧草根腐病. 国外畜牧学—草原与牧草, 1991, (2): 5-11.
[6] 南志标, 李春杰. 中国牧草真菌病害名录. 草业科学, 1994, (增刊): 1-160.
[7] 陈耀, 闵继淳, 肖风, 等. 新疆苜蓿根腐病研究初报. 中国草地, 1989, (2): 71-73.
[8] 南志标. 我国的苜蓿病害及其综合防治体系. 动物科学与动物医学, 2001, 18(4): M1-M4.
[9] 袁庆华. 我国苜蓿病害研究进展. 植物保护, 2007, 33(1): 6-12.
[12] 魏崃, 李馨园, 唐晓飞, 等. 大豆品种对立枯丝核菌的抗性研究. 大豆科学, 2012, 31(3):457-461.
[14] 张中义, 冷怀琼, 张志铭, 等. 植物病原真菌学[M]. 成都: 四川科学技术出版社, 1998: 347-348.
[15] 彭绍裘, 曾昭瑞, 张志光. 水稻纹枯病及其防治[M]. 上海: 上海科技出版社, 1986: 46-79.
[16] 邓先明. 四川省西北部稻区水稻纹枯病菌菌系研究. 西南农业大学学报, 1991,13(4): 380-383.
[18] 刘爱萍, 侯天爵. 草地病虫害及防治[M]. 北京:中国农业科学技术出版社, 2005: 28.
[21] 陈雅君, 崔国文. 黑龙江省紫花苜蓿根腐病调查及病原分离. 中国草地, 2001, 23(3): 78-79.
[22] 郭玉霞, 南志标, 李春杰, 等. 黄土高原区苜蓿与小麦轮作系统根部入侵真菌研究. 生态学报, 2004, 24(3): 486-494.
[24] 李春杰, 南志标. 苜蓿种带真菌及其致病性测定. 草业学报, 2000, 9(1): 27-36.
[27] 李敏权, 柴兆祥, 李金花, 等. 定西地区苜蓿根和根颈腐烂病病原研究. 草地学报, 2003, 21(1): 83-86.
[29] 张君成, 谢彦洁, 韦绍兴, 等. 立枯丝核菌对香蕉苗的致病力探讨. 广西农业生物科学, 2000, 19(2): 85-88.
[30] 杨金红, 白丽艳, 郭庆元, 等. 新疆奶花芸豆立枯丝核菌菌丝融合群及其致病性研究. 新疆农业科学, 2006, 43(4): 302-305.
[31] 王艳丽, 任衍春, 张震, 等. 立枯丝核菌不同融合群菌株GPD基因的克隆及序列特征分析. 中国水稻科学, 2013, 27(6): 639-646.
[32] 康霄文, 龙晓波, 彭绍裘, 等. 水稻纹枯病菌毒素的初步研究. 沈阳农业大学学报, 1992, 23(1): 19-22.
[34] 陈申宽, 姚国君. 紫苜蓿品种(种)对褐斑病抗性的研究. 草业科学, 1994, 11(3): 61-62.
[35] 李敏权. 苜蓿根和根颈腐烂病病原致病性及品种抗病性研究. 中国草地, 2003, 25(1): 39-43.
[42] 韩华雯, 孙丽娜, 姚拓, 等. 不同促生菌株组合对紫花苜蓿产量和品质的影响. 草业学报, 2013, 22(5): 104-112.
[44] 陈丹梅, 陈晓明, 梁永江, 等. 种植模式对土壤酶活性和真菌群落的影响. 草业学报, 2015, 24 (2): 77-84.