羊草茎在奶牛瘤胃中降解特性及其对食糜纤维分解菌数量的影响

doi:10.11686/cyxb2015298

草业学报 ›› 2016, Vol. 25 ›› Issue (4): 166-171.DOI: 10.11686/cyxb2015298

羊草茎在奶牛瘤胃中降解特性及其对食糜纤维分解菌数量的影响

徐俊^{1, 2}, 侯玉洁^{1, 2}, 赵国琦^{2*, *}, 罗林广¹

1. 江西省农业科学院农产品质量安全与标准研究所,江西南昌 330200;
2. 扬州大学动物科学与技术学院,江苏扬州 225009

收稿日期:2015-06-10 出版日期:2016-04-20 发布日期:2016-04-20
作者简介:徐俊(1986-),男,江西南昌人,助理研究员。E-mail:xujun0125@163.com
基金资助:
十二五国家科技支撑计划(2011BAD17B03)和国家自然科学基金(31560647)资助

Kinetics of in situ degradation of Leymus chinensis stems in the dairy cow rumen and changes in cellulolytic bacteria in digesta

XU Jun^{1, 2}, HOU Yu-Jie^{1, 2}, ZHAO Guo-Qi^{2, *}, LUO Lin-Guang¹

1.Institute of Quality Safety and Standards of Agricultural Products, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China;
2.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2015-06-10 Online:2016-04-20 Published:2016-04-20

摘要/Abstract

摘要： 本试验旨在研究羊草茎降解特性和紧密吸附于茎的3种主要纤维分解菌的动态变化。选用羊草茎为试验材料,将其纵切6份后装入尼龙袋投入瘤胃中,分别在6,12,24,48和72 h取出,利用扫描电子显微镜观察超微结构变化;取粉碎后羊草茎进行尼龙袋试验,分别在0.5,2,6、12,24,48和72 h取出,测定不同时间点中性洗涤纤维(neutral detergent fiber,NDF)降解率和吸附在茎中3种主要纤维分解菌的数量变化。结果表明,薄壁组织和韧皮部可被瘤胃微生物降解,维管束会伴随薄壁组织的降解而发生脱落。羊草茎和食糜不同时间点纤维分解菌数量均为产琥珀酸丝状杆菌>白色瘤胃球菌>黄色瘤胃球菌,茎中产琥珀酸丝状杆菌和黄色瘤胃球菌数量在24 h达峰值,分别为10⁹和10⁵ copy/g羊草茎,白色瘤胃球菌在12 h达峰值,为10⁸ copy/g羊草茎。瘤胃食糜中3种纤维分解菌的数量在24 h内基本处在一个恒定的水平,而羊草茎NDF降解率在72 h内逐渐提高,羊草NDF降解率与瘤胃食糜中3种纤维分解菌数量不同步,这可能与纤维分解菌分泌的酶活力存在滞后有关。

Abstract: In this study, we investigated the degradation characteristics of Leymus chinensis stems in the dairy cow rumen and evaluated the changes in cellulolytic bacteria during the degradation process. L. chinensis stems were cut and packed into six nylon bags, which were incubated for 6, 12, 24, 48, and 72 h in the rumen of a dairy cow. Changes in the ultra-structure of L. chinensis stems during digestion in the rumen were evaluated by scanning electron microscopy. L. chinensis stems incubated for 0.5, 2, 6, 12, 24, 48, and 72 h in the rumen were evaluated to determine their neutral detergent fiber (NDF) digestibility. The cellulolytic bacteria in these digesta samples were also analyzed. The results showed that the non-lignified parenchymal tissue and phloem were quickly degraded and the vascular bundle was released from the plant tissues after degradation of the parenchymal tissue. The main bacterial species attached to the stem tissues were Fibrobacter succinogenes>Ruminococcus albus>Ruminococcus flavefaciens. The numbers of these bacteria peaked at 24 h (10⁹/g dry matter for F. succinogenes and 10⁵/g for R. flavefaciens) or 12 h (10⁸/g DM for R. albus) of digestion in the rumen. The numbers of cellulolytic bacteria in digesta remained constant after 24 h, while the disappearance of NDF from the L. chinensis stems showed a linear increase up to 72 h of digestion. These results suggest that the disappearance of NDF from the L. chinensis stem is not synchronized with changes in the populations of cellulolytic bacteria in digesta, and may instead be related to a delayed increase in the activities of fibrolytic enzymes.

徐俊, 侯玉洁, 赵国琦, 罗林广. 羊草茎在奶牛瘤胃中降解特性及其对食糜纤维分解菌数量的影响[J]. 草业学报, 2016, 25(4): 166-171.

XU Jun, HOU Yu-Jie, ZHAO Guo-Qi, LUO Lin-Guang. Kinetics of in situ degradation of Leymus chinensis stems in the dairy cow rumen and changes in cellulolytic bacteria in digesta[J]. Acta Prataculturae Sinica, 2016, 25(4): 166-171.

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羊草茎在奶牛瘤胃中降解特性及其对食糜纤维分解菌数量的影响

Kinetics of in situ degradation of Leymus chinensis stems in the dairy cow rumen and changes in cellulolytic bacteria in digesta

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