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草业学报 ›› 2016, Vol. 25 ›› Issue (4): 140-148.DOI: 10.11686/cyxb2015308

• 研究论文 • 上一篇    下一篇

内生真菌对醉马草根际土壤线虫群落的影响

郭长辉, 李秀璋, 柳莉, 曹建新, 李春杰*   

  1. 兰州大学草地农业科技学院,草地农业生态系统国家重点实验室,甘肃 兰州 730020
  • 收稿日期:2015-06-18 出版日期:2016-04-20 发布日期:2016-04-20
  • 作者简介:郭长辉(1988-),男,甘肃靖远人,在读硕士。E-mail:guozhh13@lzu.edu.cn
  • 基金资助:
    国家重点基础研究发展计划(973项目)课题(2014CB138702),长江学者和创新团队发展计划(IRT13019)资助

Effect of the Epichloё endophyte on the soil nematode community in the rhizosphere of Achnatherum inebrians

GUO Chang-Hui, LI Xiu-Zhang, LIU Li, CAO Jian-Xin, LI Chun-Jie*   

  1. College of Pastoral Agriculture Science and Technology, State Key Laboratory of Grassland Agro-ecosystems, Lanzhou University, Lanzhou 730020, China
  • Received:2015-06-18 Online:2016-04-20 Published:2016-04-20

摘要: 为明确内生真菌对醉马草根际土壤线虫群落特征的影响,于2014年6,8和10月,对带内生真菌和不带内生真菌醉马草根际土壤进行采样,利用淘洗-过筛-蔗糖离心法分离线虫,根据线虫形态学特征进行鉴定,结果表明,本研究共捕获土壤线虫11889条,分别隶属于线虫动物门2纲6目22科37属,带内生真菌醉马草根际土壤线虫个体密度高于不带内生真菌醉马草,而类群数、多样性指数(H')、均匀度指数(J)和丰富度指数(SR)均低于不带内生真菌醉马草,但处理间差异不显著,表明尽管内生真菌使醉马草根际土壤线虫个体密度有所增加,使类群数有所降低,但是内生真菌的存在对醉马草土壤线虫多样性没有影响;与不带内生真菌醉马草相比,带内生真菌醉马草根际土壤线虫MI指数显著升高,而PPI指数和PPI/MI值显著降低,表明内生真菌在一定程度上确实改变了土壤线虫功能类群组成,其中主要影响植物寄生线虫。

Abstract: The aim of this study was to determine the effect of the Epichloё endophyte on soil nematodes and their community characteristics in the rhizosphere of Achnatherum inebrians. Soil nematodes were isolated and identified from the rhizosphere of A. inebrians plants grown with (E+) or without (E-) the Epichloё endophyte. A total of 11889 soil nematodes were collected, belonging to 37 genera, 22 families, six orders, and two classes. The individual density of soil nematodes was higher in soil of E+ plants than in soil of E- plants. The generic number, Shannon-Wiener index, Pielou index, and Margalef index were lower in E+ soil than in E- soil, whereas the diversity index did not differ significantly between the two treatments. The results suggested that the Epichloё endophyte had a limited effect on the diversity of soil nematodes of A. inebrians. The maturity index (MI) of soil nematodes was significantly higher in the E+ soil than in the E- soil, whereas the plant parasite index (PPI) and PPI/MI were significantly lower in the E+ soil than in the E- soil. The presence of the Epichloё endophyte had a significant effect on MI, PPI, and PPI/MI. These results show that the Epichloё endophyte can change the composition of functional groups of soil nematodes, especially that of plant-parasitic nematodes.