鹅观草不同居群条锈病和白粉病抗性评价

doi:10.11686/cyxb2015309

摘要/Abstract

摘要： 鉴定不同居群鹅观草种质对条锈病和白粉病的抗性,为牧草及麦类作物抗病基因的选取和抗病品种选育提供遗传资源基础。本研究采用田间接种法对来自国内外的34份鹅观草种质资源(野生资源33份,品种1份)进行了成株期抗病性鉴定和评价。结果表明,种质资源抗病能力存在多样性。34份鹅观草条锈病的严重度、病叶率和病情指数的变异范围分别为11.49%~50.46%,19.85%~74.24%和2.63~37.60,变异系数分别为30.01%,26.54%和49.67%;反应型和发生程度的变异范围分别为1~4和轻发生(1级)至大发生(5级);各病害指标之间均为极显著正相关(P<0.01)。以上5个指标综合聚类分析表明:34份材料中,6份高抗(HR,占17.65%)、4份中抗(MR,占11.76%)、17份中感(MS,占50.00%)和7份高感(HS,占20.59%)。34份鹅观草白粉病的反应型、严重度和植株感病率的变异范围分别为0~4,0~60.00%和0~100%;34份材料聚类分析得知:12份高抗(HR,占35.29%)、5份中抗(MR,占14.71%)、11份中感(MS,占32.35%)和6份高感(HS,占17.65%)。对条锈病和白粉病均具有较好抗性的材料有ZY 1007和Pr 87-88 344,可作为育种材料进一步深入研究。

Abstract: Disease-resistant grasses can serve as a germplasm resource to breed resistant varieties of wheat and forage grasses. The aim of this study was to screen populations of Roegneria kamoji for their resistance to two common pathogens, stripe rust (Puccinia striiformis) and powdery mildew (Blumeria graminis). Adult plants of 34 populations of R. kamoji (33 wild germplasms, and one cultivated variety) from domestic regions or introduced from overseas were evaluated to determine their resistance to the two pathogens in field trials. The degree of disease resistance differed among the 34 populations. Among the 34 populations of R. kamoji infected by stripe rust, the disease severity ranged from 11.49% to 50.46%, the disease incidence ranged from 19.85% to 74.24%, and the disease index ranged from 2.63-37.60, with a coefficient of variation of 30.01%, 26.54%, and 49.67%, respectively. The infection type range and occurrence degree index range were 1-4 and 1-5, respectively. The disease indexes were significantly positively correlated (P<0.01). In cluster analyses based on the above indexes, the 34 populations of R. kamoji clustered into four groups: highly resistant (HR), moderately resistant (MR), moderately susceptible (MS), and highly susceptible (HS). The proportion of the 34 populations in the HR, MR, MS, and HS groups was 17.65%, 11.76%, 50.00%, and 20.59%, respectively. In the powdery mildew field trial, the range of infection type, disease severity, and ratio of diseased plants for the 34 populations of R. kamoji was 0-4, 0%-60% and 0%-100%, respectively. The proposed resistance classifications were as follows: 12 populations for HR, 5 populations for MR, 11 populations for MS, and 6 populations for HS (35.29%, 14.71%, 32.35%, and 17.65% of the populations, respectively). These results show that the accessions ZY 1007 and Pr 87-88 344 are resistant to both stripe rust and powdery mildew. Therefore, these accessions would be excellent resources for breeding disease-resistant wheat crops and forage grasses.

赵富强, 张海琴, 孙宗华, 焦振飞, 刘晓燕, 陈韦寰, 陈国跃, 周永红. 鹅观草不同居群条锈病和白粉病抗性评价[J]. 草业学报, 2016, 25(4): 149-158.

ZHAO Fu-Qiang, ZHANG Hai-Qin, SUN Zong-Hua, JIAO Zhen-Fei, LIU Xiao-Yan, CHEN Wei-Huan, CHEN Guo-Yue, ZHOU Yong-Hong. Resistance of Roegneria kamoji (Poaceae: Triticeae) populations to stripe rust and powdery mildew[J]. Acta Prataculturae Sinica, 2016, 25(4): 149-158.

参考文献

[1] Wan A M, Zhao Z H, Wu L R. Reviews of occurrence of wheat stripe rust disease in 2002 in China. Plant Protection, 2003, 29(2): 5-8.
[2] Li Z Q. The variation of wheat variety resistance to stripe rust in China and the way of its solution. Scientia Agricultura Sinica, 1980, 13(3): 72-77.
[3] Bennett F G A. Resistance to powdery mildew in wheat: a review of its use in agriculture and breeding programmes. Plant Pathology, 1984, 33: 279-300.
[4] Duan S K, Xu Y B, Wu X Y. Research progress of pathogene virulence, resistance genes and resistance breeding of wheat powdery mildew. Journal of Triticeae Crops, 2002, 22(2): 83-86.
[5] Duan X Y, Sheng B Q, Zhou Y L, et al . Monitoring of the virulence population of Erysiphe graminis f. sp. tritici . Acta Phytophylacica Sinica, 1998, 25(1): 31-36.
[6] Feng J Y, Chen G Y, Wei Y M, et al . Identification and genetic mapping of a recessive gene for resistance to stripe rust in wheat line LM168-1. Molecular Breeding, 2014, 33: 601-609.
[7] Cheng P, Xu L S, Chen X M, et al . Molecular mapping of genes Yr 64 and Yr 65 for stripe rust resistance in hexaploid derivatives of durum wheat accessions PI 331260 and PI 480016. Theoretical and Applied Genetics, 2014, 127: 2267-2277.
[8] McIntosh R A, Dubcovsky J, Rogers W J, et al . Catalogue of gene symbols for wheat: 2013-2014 supplement. Komugi-Wheat Genetic Resources Database, http://www.shigen.nig.ac.jp/wheat/komugi/, 2014.
[9] McIntosh R A, Yamazaki Y, Dubcovsky J, et al . Catalogue of gene symbols for wheat. Proceeding of the 12th International Wheat Genetics Symposium[C]. Japan: Yokohama, 2013.
[10] Gustafson G D, Shaner G. Influence of plant age on the expression of slow-mildewing resistance in wheat. Phytopathology, 1982, 72: 746-749.
[11] Chen X M, Line R F. Gene action in wheat cultivars for durable high-temperature adult-plant resistance and interactions with race-specific, seedling resistance to stripe rust caused by Puccinia striiformis . Phytopathology, 1995, 85: 567-572.
[12] Lu B R. Diversity and conservation of the Triticeae genetic resoureces. Chinese Biodiversity, 1995, 3(2): 63-68.
[13] Guo B Z. The Flora of China (9.3)[M]. Beijing: Science Press, 1987: 51-104.
[14] Yan J, Yang J L. The Biosystematics of Triticeae (4)[M]. Beijing: China Agriculture Press, 2011: 337-586.
[15] Wan Y F, Yan J, Yang J L, et al . Study on wild relatives of wheat for resistance to head scab. Acta Phytopathologica Sinica, 1997, 27(2): 107-111.
[16] Weng Y Q, Liu D J. Morphology, scab resistance and cytogenetics of intergeneric hybrids of Triticum aestivum L. with Roegneria C. Koch ( Agropyron ) species. Scientia Agricultura Sinica, 1989, 22(5): 1-8.
[17] Cainong J C, Bockus W W, Feng Y G, et al . Chromosome engineering, mapping, and transferring of resistance to Fusarium head blight disease from Elymus tsukushiensis into wheat. Theoretical and Applied Genetics, 2015, 128: 1019-1027.
[18] Zhang H Q, Zhou Y H, Fan X, et al . The high quality forage resources—— Roegneria kamoji (Triticeae: Poaceae). The Second China Grassland Agriculture Conference[C]. Beijing: China Animal Husbandry Association Branch of Grass Industry, 2012.
[19] Zhang X Q, Wu B H, Yan J, et al . Cytogenetic studies of F 1 hybrids of Roegneria magnicaespes , R. grandis and R. alashanica (Poaceae: Triticeae). Acta Prataculturae Sinica, 1999, 8(4): 23-28.
[20] Zhou Y H, Yang J L, Zheng Y L, et al . RAPD study on inter-species relationships in Roegneria (Poaceae: Triticeae). Acta Botanica Sinica, 1999, 41(10): 1076-1081.
[21] Yang C R, Zhang H Q, Zhao F Q, et al . Genome constitution of Elymus tangutorum (Poaceae: Triticeae) inferred from meiotic pairing behavior and genomic in situ hybridization. Journal of Systematics and Evolution, 2015, 53(6): 529-534.
[22] Jiang Y Y, Zeng J, Shang H S, et al . GB/T 15795-2011 Rules for Monitoring and Forecast of the Wheat Stripe Rust ( Puccinia striiformis West)[S]. Beijing: China Standard Press, 2011.
[23] Shang H S, Jiang R Z. GB/T 15795-1995 Rules for the Investigation and Forecast of Wheat Stripe Rust ( Puccinia striiformis West)[S]. Beijing: China Standard Press, 1995.
[24] Liu W C, Jiang Y Y, Zhang Y J, et al . Rules for the Investigation and Forecast of Wheat Powdery Mildew [ Blumeria graminis (DC.)Speer][S]. Beijing: China Standard Press, 2002.
[25] Dong Y C. Genepools of common wheat. Journal of Triticeae Crops, 2000, 20(3): 78-81.
[26] Xiao H J. Study on Genetic Diversity of Roegneria Kamoji Ohwi Germplasm Resources[D]. Huhehot: Chinese Academy of Agricultural Sciences, 2007.
[27] Liu J P, Zhang X Q, You M H, et al . The resistance of wild Hemarthria compressa to Puccinia spp. in southwest of China. Acta Prataculturae Sinica, 2006, 15(4): 65-70.
[28] Wang Y, Liu Y, Zhou B B, et al . An evaluation of the resistance of alfalfa cultivars to Stemphylium and Phoma leaf spot diseases. Acta Prataculturae Sinica, 2015, 24(7): 155-162.
[29] Zhu J H, Liu M D, Hua W, et al . Identification of resistance to powdery mildew of 139 barley varieties (lines) at adult and seedling stages. Journal of Triticeae Crops, 2015, 35(5): 1-8.
[30] He Z H, Lan C X, Chen X M, et al . Progress and perspective in research of adult-plant resistance to stripe rust and powdery mildew in wheat. Scientia Agricultura Sinica, 2011, 44(11): 2193-2215.
[1] 万安民, 赵中华, 吴立人. 2002年我国小麦条锈病发生回顾. 植物保护, 2003, 29(2): 5-8.
[2] 李振岐. 我国小麦品种抗条锈性丧失原因及其解决途径. 中国农业科学, 1980, 13(3): 72-77.
[4] 段双科, 许育彬, 吴兴元. 小麦白粉病菌致病毒性和抗病基因及抗病育种研究进展. 麦类作物学报, 2002, 22(2): 83-86.
[5] 段霞瑜, 盛宝钦, 周益林, 等. 小麦白粉病菌生理小种的鉴定与病菌毒性的监测. 植物保护学报, 1998, 25(1): 31-36.
[12] 卢宝荣. 小麦族遗传资源的多样性及其保护. 生物多样性, 1995, 3(2): 63-68.
[13] 郭本兆.中国植物志(9卷3分册)[M]. 北京: 科学出版社, 1987: 51-104.
[14] 颜济, 杨俊良. 小麦族生物系统学(第四卷)[M]. 北京: 中国农业出版社, 2011: 337-586.
[15] 万永芳, 颜济, 杨俊良, 等. 小麦近缘野生植物的赤霉病抗性研究. 植物病理学报, 1997, 27(2): 107-111.
[16] 翁益群, 刘大钧. 鹅观草( Roegneria C. Koch)与普通小麦( Triticum aestivum L.)属间杂种F1的形态、赤霉病抗性和细胞遗传学研究. 中国农业科学, 1989, 22(5): 1-8.
[18] 张海琴, 周永红, 凡星, 等. 优质牧草新资源——鹅观草. 2012第二届中国草业大会论文集[C]. 北京: 中国畜牧业协会草业分会, 2012.
[19] 张新全, 伍碧华, 颜济, 等. 阿拉善鹅观草、大鹅观草及大丛鹅观草杂种的细胞学研究. 草业学报, 1999, 8(4): 23-28.
[20] 周永红, 杨俊良, 郑有良, 等. 用RAPD分子标记探讨鹅观草属的种间关系. 植物学报, 1999, 41(10): 1076-1081.
[22] 姜玉英, 曾娟, 商鸿生, 等. GB/T 15795-2011 小麦条锈病测报技术规范[S]. 北京: 中国标准出版社, 2011.
[23] 商鸿生, 姜瑞中. GB/T 15795-1995 小麦条锈病测报调查规范[S]. 北京: 中国标准出版社, 1995.
[24] 刘万才, 姜玉英, 张跃进, 等. NY/T 613-2002 小麦白粉病测报调查规范[S]. 北京: 中国标准出版社, 2002.
[25] 董玉琛. 小麦的基因源. 麦类作物学报, 2000, 20(3): 78-81.
[26] 肖海峻. 鹅观草种质资源遗传多样性研究[D]. 呼和浩特: 中国农业科学院草原研究所, 2007.
[27] 刘金平, 张新全, 游明鸿, 等. 西南地区扁穗牛鞭草种质资源抗锈病能力初步研究. 草业学报, 2006, 15(4): 65-70.
[28] 王瑜, 刘怡, 周彬彬, 等. 苜蓿对匍柄霉叶斑病与茎点霉叶斑病的抗性评价研究. 草业学报, 2015, 24(7): 155-162.
[29] 朱靖环, 刘猛道, 华为, 等. 139份大麦种质材料苗期和成株期抗白粉病鉴定评价. 麦类作物学报, 2015, 35(5): 1-8.
[30] 何中虎, 兰彩霞, 陈新民, 等. 小麦条锈病和白粉病成株抗性研究进展与展望. 中国农业科学, 2011, 44(11): 2193-2215.