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草业学报 ›› 2016, Vol. 25 ›› Issue (9): 189-196.DOI: 10.11686/cyxb2015544

• 研究简报 • 上一篇    下一篇

不同产地藿香花粉活力与柱头可授性研究

苏芸芸, 王康才*, 薛启   

  1. 南京农业大学园艺学院,江苏 南京 210095
  • 收稿日期:2015-12-02 出版日期:2016-09-20 发布日期:2016-09-20
  • 通讯作者: E-mail:wangkc@njau.edu.cn
  • 作者简介:苏芸芸(1991-),女,甘肃天水人,在读硕士。 E-mail:2014104128@njau.edu.cn
  • 基金资助:
    工信部藿香规范化与规模化生产基地建设项目(2014)资助

Study of the pollen viability and stigma receptivity of Agastache rugosa from different areas

SU Yun-Yun, WANG Kang-Cai*, XUE Qi   

  1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2015-12-02 Online:2016-09-20 Published:2016-09-20

摘要: 主要对不同产地的藿香花粉活力、柱头可授性及花粉形态特征进行研究。各产地藿香花粉活力检测经过多种方法筛选后选择红墨水法,柱头可授性测定采用联苯胺-过氧化氢法,并利用扫描电镜对不同产地藿香的花粉形态进行观察。结果表明,在自然条件下,各产地开花习性较为一致,均在7至8月开花,群体花期15~28 d,单花花期约为3~5 d。不同产地藿香花粉活力随着开花时间的延长均表现出先升高后降低的趋势,平均花粉活力在18.46%~26.52%之间。5个产地当中,成都产地藿香平均花粉活力最高,长春产地藿香花粉活力最低。花粉寿命在4~5 d之间,柱头可授性可持续5~6 d。同时,在藿香刚开花第1天各产地柱头具可授性,在2~3 d内,具有较强的可授性。因而,不同产地藿香进行人工授粉最佳时期为开花后2~3 d。此外,不同产地藿香花粉粒形状主要为近球形或椭球形。花粉粒大小在42.93 μm×31.99 μm~46.59 μm×37.67 μm之间,均具6条萌发沟,萌发沟狭长、裂痕近两极。花粉外壁纹饰呈网状,网眼为不规则多角形,近圆形,大小不一。本文较为系统的比较了不同产地藿香花粉活力、柱头可授性及花粉形态,以期为藿香的人工栽培以及良种繁育提供理论依据。

Abstract: A study was undertaken to explore the pollen viability, stigma receptivity and pollen morphology of Agastache rugosa from different areas. Pollen viability was evaluated by red ink method; stigma receptivity was evaluated by benzidine-H2O2 method; pollen morphology was observed by scanning electron microscopy. The results showed that, the flowering habit was identical in the different areas under natural conditions. The majority of A. rugosa bloomed from July to August; the flowering stage of the populations was approximately 15-28 days and the life span of a single flower was about 3-5 days. The pollen viability of A. rugosa from different areas showed at first an increased and then a decreased trend with the increase of blooming time, with average pollen viability ranging between 18.46% and 26.52%. Among the five areas, the highest average pollen viability was from Chengdu and the lowest from Jilin. The life span of pollen was 4-5 days while stigma receptivity continued for 5-6 days. The stigma of A. rugosa from different areas had receptivity in the first day of blooming and higher receptivity in 2-3 days. This result indicated that the optimum artificial pollination time of different sources of A. rugosa is 2-3 days after blooming. The pollen shapes of A. rugosa from different areas were mainly spheroid and subglobose. The pollen size ranged between 42.93 μm×31.99 μm and 46.59 μm×37.67 μm. With 6 germinal furrows, the shape was long and narrow, almost reaching the poles. The surface emblazonry was netting and the shapes of pollen mesh were mostly irregular polygons to nearly round. These results provided a theoretical basis for the artificial cultivation and elite breeding of A. rugosa in the future.