草坪草红丝病与粉斑病病原菌生物学特性研究与杀菌剂室内毒力测定

doi:10.11686/cyxb2016058

草业学报 ›› 2016, Vol. 25 ›› Issue (12): 140-149.DOI: 10.11686/cyxb2016058

草坪草红丝病与粉斑病病原菌生物学特性研究与杀菌剂室内毒力测定

章武^{1, 2}, 胡美姣³, 高兆银³, 李敏³, 刘国道⁴, 南志标^{1*, *}

1.草地农业生态系统国家重点实验室,兰州大学草地农业科技学院,甘肃兰州 730020;
2.岭南师范学院,广东湛江 524048;
3.中国热带农业科学院环境与植物保护研究所,海南海口571101;
4.中国热带农业科学院热带作物品种资源研究所,海南儋州571737

收稿日期:2016-02-01 修回日期:2016-06-06 出版日期:2016-12-20 发布日期:2016-12-20
通讯作者: zhibiao@lzu.edu.cn
作者简介:章武(1987-),男,江西抚州人,讲师。E-mail:ldzw1987@163.com
基金资助:
国家公益性行业农业科技专项(201303057),国家自然科学基金(31602002)和广东省教育厅重点平台及科研项目——青年创新人才类项目(2015KQNCX093)资助

Biological characteristics of Laetisaria fuciformis and Limonomyces roseipellis and their responses to different fungicides

ZHANG Wu^{1, 2}, HU Mei-Jiao³, GAO Zhao-Yin³, LI Min³, LIU Guo-Dao⁴, NAN Zhi-Biao^{1, *}

1.State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
2.Lingnan Normal College, Zhanjiang 524048, China;
3.Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;
4.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

Received:2016-02-01 Revised:2016-06-06 Online:2016-12-20 Published:2016-12-20

摘要/Abstract

摘要： 草坪草红丝病与粉斑病是发生于世界范围内可危害多个草种的重要草坪草病害。本研究对我国首次报道的红丝病和粉斑病病原菌生物学特性进行了研究并在室内测定了14种杀菌剂对该两种病原菌的毒力。研究结果表明:红丝病和粉斑病病原菌菌丝适宜生长pH为5~7,最适生长pH为6,菌丝的致死温度为50 ℃水浴处理10 min。红丝病和粉斑病病原菌能够利用多种碳氮源,最适碳源和最适氮源分别为可溶性淀粉和L-谷氨酰胺。红丝病病原菌最适培养基为燕麦片煎液琼脂培养基(OMA);粉斑病病原菌最适培养基为马铃薯葡萄糖琼脂培养基(PDA)和马铃薯蔗糖琼脂培养基(PSA)。5%烯唑醇在14种供试杀菌剂中对红丝病病原菌和粉斑病病原菌毒力最高,其EC₅₀值分别为0.113和0.282 mg/L;然而,15%甲霜灵·10%霜霉威毒力最低,其EC₅₀值分别为121.522和214.532 mg/L。研究结果为深入了解病原菌的基础生物学及制定防治策略提供科学依据。

Abstract: Red thread and pink patch, caused by the fungi Laetisaria fuciformis and Limonomyces roseipellis, respectively, are important diseases of many turfgrass species worldwide. In this study, we analyzed the biological characteristics of these two pathogens and evaluated their responses to 14 different fungicides. The results showed that L. fuciformis and Li. roseipellis grew well at pHs ranging from 5 to 7, and the optimal pH value was 6. The hyphae of both pathogens lost viability in agar plugs that were incubated at 50 ℃ for 10 min. The optimal carbon and nitrogen sources for L. fuciformis and Li. roseipellis were soluble starch and L-glutamine, respectively. The optimal medium for L. fuciformis was oatmeal agar (OMA) and that for Li. roseipellis was potato dextrose agar (PDA) or potato sucrose agar (PSA). Among the 14 fungicides, diniconazole had the strongest inhibitory effects on L. fuciformis and Li. roseipellis with EC₅₀ values of 0.113 mg/L and 0.282 mg/L, respectively. The fungicide with the weakest inhibitory effects was 15% metalaxyl·10% propamocarb, with EC₅₀ values of 121.522 and 214.532 mg/L for L. fuciformis and Li. roseipellis, respectively. The results provide a scientific basis for understanding the basic biology of these pathogens, and may be useful for the development of new management strategies.

章武, 胡美姣, 高兆银, 李敏, 刘国道, 南志标. 草坪草红丝病与粉斑病病原菌生物学特性研究与杀菌剂室内毒力测定[J]. 草业学报, 2016, 25(12): 140-149.

ZHANG Wu, HU Mei-Jiao, GAO Zhao-Yin, LI Min, LIU Guo-Dao, NAN Zhi-Biao. Biological characteristics of Laetisaria fuciformis and Limonomyces roseipellis and their responses to different fungicides[J]. Acta Prataculturae Sinica, 2016, 25(12): 140-149.

参考文献

[1] Xu B L. The Lawn Protection[M]. Beijing: Chinese Forestry Press, 2013.
[2] Nan Z B, Li C J. The directory of forage grasses diseases in China. Pratacultural Science, 1994, 13(Supplementary issue): 1-160.
[3] Lv C, Luo L, Hsiang T. First report of dollar spot of seashore paspalum ( Paspalum vaginatum ) caused by Sclerotinia homoeocarpa in South China. Plant Disease, 2010, 94(3): 373.
[4] Zhang W, Nan Z B, Liu G D, et al . First report of leaf and sheath spot caused by Waitea circinat avar. zeae on Paspalum vaginatum and Zoysia tenuifolia in China. Plant Disease, 2014, 98(10): 1436.
[5] Zhang W, Liu G D, Nan Z B. Identification and biological charactertistics of Sclerotinia homoeoparpa causing dollar spot on 4 warm-season turfgrass. Acta Prataculturae Sinica, 2015, 24(1): 124-131.
[6] Zhang W, Nan Z B, Tian P, et al . Microdochium paspali , a new species causing seashore paspalum disease in south China. Mycologia, 2015, 107(1): 80-89.
[7] Couch H B. Disease of Turfgrass[M]. Malabar, F L: Krieger Publishing Company, 1995.
[8] Kaplan J D, Jackson N. Red thread and pink patch diseases of turfgrasses. Plant Disease, 1983, 67(2): 159-162.
[9] Smiley R W, Dernoeden P H, Clarke B B. Compendium of Turf grass Diseases[M]. St. Paul: American Phytopathological Society (APS Press), 2005.
[10] Stalpers J A, Loerakker W M. Laetisaria and Limonomyces species (Corticiaceae) causing pink diseases in turfgrasses. Canadian Journal of Botany, 1982, 60(5): 529-537.
[11] Maccaroni M, Corazza L, Buonaurio R, et al . Occurrence of pink patch of perennial ryegrass caused by Limonomyces roseipellis in Italy. Plant Disease, 2002, 86(1): 74.
[12] Burdsall H H. Laetisaria (Aphyllophorales, Corticiaceae), a new genus for the teleomorph of Isaria fuciformis . Transaction of the British Mycological Society, 1979, 72(3): 419-422.
[13] Zhang W, Nan Z B, Liu G D. First report of Laetisaria fuciformis causing red thread on seashore paspalum ( Paspalum vaginatum ) in south China. Plant Disease, 2012, 96(9): 1374.
[14] Zhang W, Nan Z B, Liu G D. First report of Limonomyces roseipellis causing pink patch on bermudagrass in south China. Plant Disease, 2013, 97(4): 561.
[15] Zhang W, Hu M J, Liu G D, et al . Investigation and characterization of red thread and pink patch on warm-season turfgrasses in Hainan Province, tropical China. European Journal of Plant Pathology, 2015, 141(2): 311-325.
[16] Li Y Z, Nan Z B. Nutritional study on Embellisia astragali , a fungal pathogen of milk vetch ( Astragalus adsurgens ). Antonie van Leeuwenhoek, 2009, 95(3): 275-284.
[17] Gould C J, Goss R L, Vassey W E. Fungicidal tests for control of Corticium red thread in the Pacific North West. Journal of the Sports Turf Research Institute, 1976, 51: 62-66.
[18] Koch P L, Grau C R, Jo Y K, et al . Thiophanate-methyl and propiconazole sensitivity in Sclerotinia homoeocarpa populations from golf courses in Wisconsin and Massachusetts. Plant Disease, 2009, 93(1): 100-105.
[19] Young J R, Tomaso-Peterson M, De la Cerda K, et al . Two mutations in β-tubulin 2 gene associated with thiophanate-methyl resistance in Colletotrichum cereale isolates from creeping bentgrass in Mississippi and Alabama. Plant Disease, 2010, 94(2): 207-212.
[20] Wong F P, De la Cerda K A, Hernandez-Martinez R, et al . Detection and characterization of benzimidazole resistance in California populations of Colletotrichum cereale . Plant Disease, 2008, 92(2): 239-246.
[21] Putman A I, Jung G, Kaminski J E. Geographic distribution of fungicide-insensitive Sclerotinia homoeocarpa isolates from golf courses in the northeastern United States. Plant Disease, 2010, 94(2): 186-195.
[22] Warren C G, Sanders P, Cole H J, et al . Relative fitness of benzimidazole- and cadmium-tolerant populations of Sclerotinia homoeocarpa in the absence and presence of fungicides. Phytopathology, 1977, 67: 704-708.
[23] Warren C G, Sanders P, Cole H. Sclerotinia homoeocarpa tolerance to benzimidazole configuration fungicides. Phytopathology, 1974, 64: 1139-1142.
[24] Detweiler A R, Vargas Jr J M, Danneberger T K. Resistance of Sclerotinia homoeocarpa to iprodione and benomyl. Plant Disease, 1983, 67(6): 627-630.
[25] Wang J H, Duo D. Effect of paclobutrazol on drought resistance of six turfgrass cultivars during the seeding stage. Acta Prataculturae Sinica, 2014, 23(6): 253-258.
[26] Liu Q, Yao T, Ma H L. Combined effects of bio-fertilizer and citric acid on turf quality and soil biology on a calcareous soil. Acta Prataculturae Sinica, 2014, 23(5): 223-230.
[27] Li X Y, Wang Y R, Jia C Z. Effect of urea fertilizer on growth and performance of Cleistogenes songorica turfgrass. Acta Prataculturae Sinica, 2014, 23(6): 136-141.
[28] Woolhouse A R. Assessment of perennial ryegrass cultivars for susceptibility to red thread disease. Journal of the Sports Turf Research Institute, 1996, 67: 147-152.
[29] Raikes C, Leep N W, Canaway P M. The effect of red thread ( Laetisaria fuciformis ) incidence on wear tolerance of monostands of perennial ryegrass ( Lolium perenne ) cultivars under three nitrogen. Journal of the Sports Turf Research Institute, 1996, 72: 61-66.
[30] Cahill J V, Murray J J, Dernoeden P H. Interrelationships between fertility and red thread fungal disease of turf grasses. Plant Disease, 1983, 67(10): 1080-1083.
[31] Tredway L P, Soika M D, Clarke B B. Red thread development in perennial ryegrass in response to nitrogen, phosphorus, and potassium fertilizer applications. International Turfgrass Society Research Journal, 2001, 9: 715-721.
[1] 徐秉良. 草坪保护学[M]. 北京: 中国林业出版社, 2013.
[2] 南志标, 李春杰. 中国牧草真菌病害名录. 草业科学, 1994, 13(增刊): 1-160.
[5] 章武, 刘国道, 南志标. 4种暖季型草坪草币斑病病原菌鉴定及其生物学特性. 草业学报, 2015, 24(1): 124-131.
[25] 王竞红, 多多. 多效唑对6种草坪草苗期抗旱性影响的研究. 草业学报, 2014, 23(6): 253-258.
[26] 刘强, 姚拓, 马晖玲. 菌肥与柠檬酸互作对石灰性土壤生物学特性及草坪质量的影响. 草业学报, 2014, 23(5): 223-230.
[27] 李欣勇, 王彦荣, 贾存智. 施尿素对无芒隐子草草坪生长特性的影响. 草业学报, 2014, 23(6): 136-141.

草坪草红丝病与粉斑病病原菌生物学特性研究与杀菌剂室内毒力测定

Biological characteristics of Laetisaria fuciformis and Limonomyces roseipellis and their responses to different fungicides

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics