多效唑作用下紫穗槐对干旱胁迫的生理响应及抗旱性评价

doi:10.11686/cyxb2016138

摘要/Abstract

摘要： 为了探究多效唑作用下紫穗槐对干旱胁迫的响应,采用盆栽方法研究6水平多效唑下(D₀~D₅依次为0,10,50,100,150,200 mg/L)紫穗槐在不同干旱程度下的过氧化物酶(POD)、丙二醛(MDA)、可溶性蛋白、可溶性糖、叶绿素、叶片保水力指标,用隶属函数法进行抗旱性综合评价。结果表明,随着干旱程度的加深,POD、MDA、叶片失水率呈上升趋势;可溶性蛋白呈下降趋势;除D₀外,其余多效唑水平叶绿素含量上升;可溶性糖总体上升。轻度和重度干旱胁迫时,D₅的POD值最大,D₂和D₃的MDA值最小,D₄的可溶性蛋白、叶绿素、可溶性糖含量、叶片保水力最高。隶属函数分析得紫穗槐的抗旱性强弱顺序为 D₄>D₅>D₃>D₂>D₁>D₀,施加多效唑显著提高了紫穗槐的抗旱性,最适的多效唑水平为D₄。灰色关联分析得到多效唑下紫穗槐的可溶性蛋白、叶绿素、可溶性糖含量与抗旱性联系最紧密,可作为抗旱性的代表指标。研究结果可为多效唑在紫穗槐的岩石边坡防护中的推广应用提供参考。

Abstract: A study has been undertaken to investigate the response of Amorpha fruiticosa to drought stress under different paclobutrazol applications. Physiological parameters, including peroxidase (POD) activity, the contents of malondialdehyde (MDA), soluble protein, soluble sugar and chlorophyll, and the ability of leaves to conserve water, were measured using pot experiments with six levels of paclobutrazol application (0, 10, 50, 100, 150 and 200 mg/L, represented by D₀, D₁, D₂, D₃, D₄ and D₅ respectively). Drought resistance was investigated using subordinate function analysis. With increasing drought stress, POD activity, MDA content and the rate of leaf water loss increased while soluble protein content decreased. Chlorophyll content increased except under D₀ and soluble sugar content increased overall. Under mild and severe drought stress, POD activity under D₅ was highest, MDA contents under D₂ and D₃ were lowest, and the contents of soluble protein, chlorophyll, soluble sugar and leaf water conservation ability under D₄ were greatest. The order of drought resistance was D₄>D₅>D₃>D₂>D₁>D₀, suggesting that the resistance of A. fruiticosa is elevated by paclobutrazol application and that D₄ is the optimum application level. Grey correlation analysis showed that soluble protein, chlorophyll and soluble sugar were closely correlated with drought resistance under paclobutrazol application, suggesting that they could be key indicators in evaluating resistance under such applications. This study provides references for the utilization of paclobutrazol applications for rock slope protection with plantings of A. fruiticosa.

范志霞, 李绍才, 孙海龙. 多效唑作用下紫穗槐对干旱胁迫的生理响应及抗旱性评价[J]. 草业学报, 2017, 26(3): 132-141.

FAN Zhi-Xia, LI Shao-Cai, SUN Hai-Long. Physiological response of Amorpha fruiticosa to drought stress under paclobutrazol application and an evaluation of drought resistance[J]. Acta Prataculturae Sinica, 2017, 26(3): 132-141.

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