高温胁迫对高羊茅航天诱变新品系生理特性研究及综合评价

doi:10.11686/cyxb2016157

草业学报 ›› 2017, Vol. 26 ›› Issue (3): 121-131.DOI: 10.11686/cyxb2016157

高温胁迫对高羊茅航天诱变新品系生理特性研究及综合评价

李娟, 雷霞, 钟理, 王小利, 杨春燕, 吴佳海^*

贵州省草业研究所,贵州贵阳 550006

收稿日期:2016-04-07 修回日期:2016-06-29 出版日期:2017-03-20 发布日期:2017-03-20
通讯作者: *1848266168@qq.com
作者简介:李娟(1982-),女,甘肃平凉人,副研究员,硕士。E-mail:xixingyue@126.com
基金资助:
贵州省联合基金(黔科合J字LKN[2013]24号),贵州省重大专项(黔科合重大专项字[2014]6017号)和贵州省高层次创新型人才培养(黔科合人才[2016]4024号)资助

Physiological response of new strains of Festuca arundinacea mutated in space under high temperature stress and comprehensively evaluated

LI Juan, LEI Xia, ZHONG Li, WANG Xiao-Li, YANG Chun-Yan, WU Jia-Hai^*

Guizhou Institute of Prataculture, Guiyang 550006, China

Received:2016-04-07 Revised:2016-06-29 Online:2017-03-20 Published:2017-03-20

摘要/Abstract

摘要： 本研究以高羊茅航天诱变新品系和贵州主推的育成品种水城高羊茅和黔草1号高羊茅共11份材料为研究对象,采用盆栽试验,在42 ℃/32 ℃(12 h/12 h)人工气候室进行高温胁迫试验,每4 d测定可溶性蛋白、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性、过氧化氢酶(CAT)生理生化指标,并应用隶属函数法和灰色关联度法对其生理指标及11份高羊茅材料耐热性进行综合评价。结果表明,可溶性蛋白质随着高温胁迫时间的延长总体呈下降趋势,SOD、POD、CAT含量随着高温时间的延长总体呈上升趋势。4个生理指标在11份材料间均存在显著性差异。利用隶属函数法对11份高羊茅材料进行综合耐热适应性评价,得出耐热性顺序为:SP5-85>SP5-60>SP5-71>SP5-42>SP5-94>SP5-7>SP5-88>水城高羊茅>SP5-5>SP5-89=黔草1号高羊茅。利用灰色关联性分析法得出各耐热指标与耐热性的关联序为:SOD>CAT>POD>可溶性蛋白质。

Abstract: The influence of high temperature stress on the eco-physiological characteristics of 11 Festuca arundinacea was studied in a pot experiment for 4 days. Physiological and biochemical indexes, including soluble protein content, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity, were measured and analyzed. Subordinate function and grey correlative analyses were used to comprehensively evaluate the 11 F. arundinacea materials under high temperature stress. The results showed that the content of soluble protein decreased, while SOD, POD and CAT contents showed an upward trend. The high temperature resistance of the 11 F. arundinacea was obtained by subordinate function. The resistance order is: SP5-85>SP5-60>SP5-71>SP5-42>SP5-94>SP5-7>SP5-88>F. arundinacea cv Shuicheng>SP5-5>SP5-89=F. arundinacea cv Qiancao No.1. The high resistance correlative order obtained by grey correlative analysis is SOD>CAT>POD>content of soluble protein.

李娟, 雷霞, 钟理, 王小利, 杨春燕, 吴佳海. 高温胁迫对高羊茅航天诱变新品系生理特性研究及综合评价[J]. 草业学报, 2017, 26(3): 121-131.

LI Juan, LEI Xia, ZHONG Li, WANG Xiao-Li, YANG Chun-Yan, WU Jia-Hai. Physiological response of new strains of Festuca arundinacea mutated in space under high temperature stress and comprehensively evaluated[J]. Acta Prataculturae Sinica, 2017, 26(3): 121-131.

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高温胁迫对高羊茅航天诱变新品系生理特性研究及综合评价

Physiological response of new strains of Festuca arundinacea mutated in space under high temperature stress and comprehensively evaluated

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