结缕草ZjCSD基因的克隆及表达分析

doi:10.11686/cyxb2016260

草业学报 ›› 2017, Vol. 26 ›› Issue (2): 102-110.DOI: 10.11686/cyxb2016260

结缕草ZjCSD基因的克隆及表达分析

张雪¹, 孙鑫博², 樊波¹, 张胤冰¹, 韩烈保¹, 许立新^{1*, *}

1.北京林业大学草坪研究所,北京 100083;
2.河北省作物生长调控重点实验室,河北农业大学,河北保定 071001

收稿日期:2016-06-27 出版日期:2017-02-20 发布日期:2017-02-20
通讯作者: lixinxu@bjfu.edu.cn
作者简介:张雪(1991-),女,山东烟台人,在读硕士。E-mail:zhangxuenearyuki@163.com
基金资助:
中国林学会——青年人才托举工程项目和国家高新技术研究发展计划项目(2013AA102607)资助

Molecular cloning and expression analysis of ZjCSD from Zoysia japonica

ZHANG Xue¹, SUN Xin-Bo², FAN Bo¹, ZHANG Yin-Bing¹, HAN Lie-Bao¹, XU Li-Xin^{1, *}

1.Institute of Turfgrass Science, Beijing Forestry University, Beijing 100083, China;
2.Key Laboratory of Crop Growth Regulation of Hebei Province, Agricultural University of Hebei, Baoding 071001, China

Received:2016-06-27 Online:2017-02-20 Published:2017-02-20

摘要/Abstract

摘要： 铜锌超氧化物歧化酶是植物响应逆境胁迫过程中的关键酶,其含量和活性与植物抗逆性密切相关。本研究以结缕草cDNA为模板,利用同源克隆法,从结缕草转录组数据库中克隆获得了结缕草ZjCSD基因,该基因编码一个含有152个氨基酸的蛋白质。生物信息学分析结果显示:ZjCSD基因编码蛋白为稳定的、亲水的、酸性、非分泌脂溶蛋白,定位于细胞质中,含有CSD蛋白家族特有的保守结构域,具有典型的Cu²⁺和Zn²⁺结合位点;与小米、玉米等禾本科植物具有较高的同源性,进化关系较近。采用实时荧光定量PCR研究该基因在不同组织中、不同胁迫处理下的表达模式,结果表明,ZjCSD基因在根、茎、叶中都有表达,叶中表达量最高;干旱胁迫(30% PEG)、盐胁迫(150 mmol/L NaCl)和Cd²⁺胁迫(200 mg/L Cd²⁺)均能诱导ZjCSD基因表达量上调,Pb²⁺胁迫(1 g/L Pb²⁺)诱导ZjCSD基因表达量下调。故推测结缕草ZjCSD基因在结缕草应对干旱、盐和重金属胁迫的过程中发挥作用。

Abstract: Copper/zinc-superoxide dismutase (CSD) is a key enzyme involved in the plant response to abiotic stress. Its content and activity is closely related to the stress tolerance of plants. The ZjCSD gene was isolated from the transcriptome database of Zoysia japonica by homologous cloning. It encoded a protein of 152 amino acid residues. Bioinformatics analysis revealed that the protein encoded by ZjCSD gene was stable, hydrophobic, acidic, fat-soluble, and located in the cytoplasm. With typical Cu²⁺ and Zn²⁺binding sites, ZjCSD belonged to the plant SOD super family. A homology analysis based on the deduced amino sequence indicated that ZjCSD had a closer relationship with CSD from Setaria italica and Zea mays than with CSDs from other plants. The expression profiles of ZjCSD in different tissues and under different stress treatments were investigated by qRT-PCR. Transcripts of ZjCSD were detected in the root, stem, and leaf. The highest transcript levels were in the leaf. The ZjCSD mRNA levels were up-regulated by salt, drought, and cadmium stress, and down-regulated by lead stress. These results suggested that ZjCSD might play a role in drought, salt, and heavy metal stress tolerance in Z. japonica.

张雪, 孙鑫博, 樊波, 张胤冰, 韩烈保, 许立新. 结缕草ZjCSD基因的克隆及表达分析[J]. 草业学报, 2017, 26(2): 102-110.

ZHANG Xue, SUN Xin-Bo, FAN Bo, ZHANG Yin-Bing, HAN Lie-Bao, XU Li-Xin. Molecular cloning and expression analysis of ZjCSD from Zoysia japonica[J]. Acta Prataculturae Sinica, 2017, 26(2): 102-110.

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结缕草ZjCSD基因的克隆及表达分析

Molecular cloning and expression analysis of ZjCSD from Zoysia japonica

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