新疆狗牙根种质芽期耐盐性综合评价

doi:10.11686/cyxb2016428

摘要/Abstract

摘要： 为了解不同狗牙根种质在芽期耐盐差异程度,采用常规纸上萌发法,研究不同NaCl浓度(0、0.4%、0.8%、1.2%、1.6%)对来源于新疆不同生境下50份狗牙根种质相对发芽率、相对发芽势、发芽指数、胚根长、胚芽长的影响,并通过聚类分析法对其耐盐性进行综合评价。结果表明,随盐胁迫增加,狗牙根种质相对发芽率、相对发芽势、发芽指数呈明显降低趋势,而胚根长、胚芽长则呈先升后降趋势,0.4%盐胁迫下达到最高;各测试指标变异系数呈逐渐加大趋势,至1.6%盐胁迫时其变异系数均较对照增加2.0倍以上。新疆狗牙根半致死NaCl浓度(S_50%)均值为1.28%,变异系数为45.66%,且1.2%NaCl可作为其耐盐鉴定最适浓度。聚类分析综合表明,50份新疆狗牙根种质中表现较稳定的耐盐种质为Cd012,中度耐盐种质为Cd019、Cd021、Cd020、Cd054,敏盐种质为Cd001、Cd033、Cd035、Cd057、Cd059。

Abstract: The aim of these experiments was to determine the differences in salt resistance among Cynodon dactylon germplasm collected from Xinjiang. The relative germination rate, relative germination energy, germination index, and the length of the radical and plumule of 50 C. dactylon accessions were measured with the paper germination method under 0 (control), 0.4%, 0.8%, 1.2%, and 1.6% NaCl stress. Then, a synthetic evaluation of salt-resistance was conducted using the cluster analysis method. As the salt concentration increased, the relative germination rate, relative germination energy, and germination index of C. dactylon significantly decreased, and the length of the radical and plumule first increased then decreased, and peaked at 0.4% NaCl. As the salt concentration increased, the variation coefficient of all indexes increased. The variation coefficient under 1.6% salt stress was more than 2.0 times that in the control (no NaCl). The semi-lethal NaCl concentration for the C. dactylon germplasm was 1.28% (variation coefficient, 45.66%) and 1.2% NaCl was the optimum concentration to evaluate the salt tolerance of C. dactylon. The comprehensive cluster analysis of 50 Xinjiang C. dactylon accessions identified Cd012 as the most salt-tolerant accession; Cd019, Cd021, Cd020, and Cd054 as moderately salt-tolerant accessions; and Cd001, Cd033, Cd035, Cd057, and Cd059 as salt-sensitive accessions.

柴艳, 孙宗玖, 李培英, 巴德木其其格, 张向向, 杨静. 新疆狗牙根种质芽期耐盐性综合评价[J]. 草业学报, 2017, 26(8): 154-167.

CHAI Yan, SUN Zong-Jiu, LI Pei-Ying, BADEMU Qiqige, ZHANG Xiang-Xiang, YANG Jing. Evaluation of the salt resistance of the Cynodon dactylon germplasm from Xinjiang during the seed germination period[J]. Acta Prataculturae Sinica, 2017, 26(8): 154-167.

参考文献

[1] Munns R. Genes and salt tolerance: Bringing them together. New Phytologist, 2005, 167(3): 645-663.
[2] Kovda V A. Loss of productive land due to salinazation. Ambio, 1983, 12(2): 91-93.
[3] Wang Z Q. Salt-Affected Soils of China[M]. Beijing: Science Press, 1993.
王遵亲. 中国盐渍土[M]. 北京: 科学出版社, 1993.
[4] Liu C H, Su J K, Huang W H. Studies on the salt-tolerant forage grass cultivars. Grassland of China, 1992, (6): 12-17, 22.
刘春华, 苏加楷, 黄文惠. 禾本科牧草耐盐性的研究. 中国草地, 1992, (6): 12-17, 22.
[5] Ai W N E. Discussion on exploitation of water resource and construction of ecological environmental protection in Xinjiang. Arid Environmental Monitoring, 2001, (3): 11-15.
艾瓦努尔. 新疆水资源开发与生态环境保护建设初探. 干旱环境监测, 2001, (3): 11-15.
[6] Xinjiang Wasteland Resources Comprehensive Expedition Community. Reasonable Utilization of Wasteland Resources in Main Areas Xinjiang[M]. Urumqi: Xinjiang People Press, 1985.
新疆荒地资源综合考察队. 新疆重点地区荒地资源合理利用[M]. 乌鲁木齐: 新疆人民出版社, 1985.
[7] Xi J B, Zang F S, Mao D R, et al . Saline-soil distribution and halophyte resources in Xinjiang. Chinese Journal of Soil Science, 2005, (3): 299-303.
郗金标, 张福锁, 毛达如, 等. 新疆盐渍土分布与盐生植物资源. 土壤通报, 2005, (3): 299-303.
[8] Simone M, Monica G, Lisa C, et al . Phenotypic traits and establishment speed of 44 turf bermudagrass accessions. Acta Agriculturea Scandinavica, Section B: Soil & Plant Science, 2014, 64(8): 722-733.
[9] Wang H L. Study on Salt-Resistance Comparision of Bermudagrass Under Salt Stress of Na 2 SO 4 [D]. Urumqi: Xinjiang Agricultural University, 2003
王红玲. Na 2 SO 4 盐胁迫下狗牙根抗盐性比较研究[D]. 乌鲁木齐: 新疆农业大学, 2003.
[10] Ling L, Zhang X Q, Liu W. Research advances on genetic diversity in wild Cynodon dactylon germplasm. Pratacultural Science, 2012, 29(6): 993-996.
凌瑶, 张新全, 刘伟. 野生狗牙根种质资源的遗传多样性研究简述. 草业科学, 2012, 29(6): 993-996.
[11] Zheng Y Q, Zang G C, Li Y T, et al . A study on the phenotypic diversity of Cynodon dactylon . Acta Agrestia Sinica, 2015, 23(3): 557-563.
郑轶琦, 臧国长, 李玉恬, 等. 狗牙根种质资源表型遗传多样性分析. 草地学报, 2015, 23(3): 557-563.
[12] Huang C Q, Liu G D, Bai C J, et al . A study on the morphological diversity of 475 accessions of Cynodon dactylon . Acta Prataculturae Sinica, 2012, 21(4): 33-42.
黄春琼, 刘国道, 白昌军, 等. 475份狗牙根种质资源形态多样性的研究. 草业学报, 2012, 21(4): 33-42.
[13] Yang Y, Lou Y H, Yang Z J, et al . Effect of low temperature on phytohormones and carbohydrates metabolism in Bermuda grass. Acta Prataculturae Sinica, 2016, 25(2): 205-215.
杨勇, 娄燕宏, 杨知建, 等. 低温胁迫对狗牙根激素和碳水化合物代谢的影响. 草业学报, 2016, 25(2): 205-215.
[14] Duan M M, Sun Z J, Li P Y, et al . Evaluation of drought resistance of 50 Cynodon dactylon germplasms at germinating stage under PEG stress. Chinese Journal of grassland, 2015, 37(4): 27-34.
段敏敏, 孙宗玖, 李培英, 等. PEG 胁迫下50份新疆狗牙根种质芽期抗旱性鉴定. 中国草地学报, 2015, 37(4): 27-34.
[15] Chan Z L, Shi H T, Wang Y P. Response of bermuda grass to abiotic stress. Pratacultural Science, 2013, 30(8): 1182-1187.
产祝龙, 施海涛, 王艳平. 狗牙根抗非生物胁迫的研究进展. 草业科学, 2013, 30(8): 1182-1187.
[16] Chen J B, Liu J X. Salinity tolerance evaluation and mechanisms in bermudagrass ( Cynodon spp). Acta Prataculturae Sinica, 2012, 21(5): 302-310.
陈静波, 刘建秀. 狗牙根抗盐性评价及抗盐机理研究进展. 草业学报, 2012, 21(5): 302-310.
[17] Marcum K B, Pessarakli M. Salinity tolerance and salt gland excretion efficiency of bermudagrass turf cultivars. Crop Science, 2006, 46: 2571-2574.
[18] Bauer B K, Poulter R E, Troughton A D, et al . Salinity tolerance of twelve hybrid bermudagrass [ Cynodon dactylon (L.) Pers× C. transvaalensis Burtt Davy] genotypes. International Turfgrass Society Research Journal, 2009, 11: 313-326.
[19] Chen J B, Zong J Q, Gao Y Z, et al . Genetic variation of salinity tolerance in Chinese natural bermudagrass ( Cynodon dactylon (L.) Pers.) germplasm resources. Acta Agriculturae Scandinavica, Section B: Soil & Plant Science, 2014, 64(5): 416-424.
[20] Zhou X, Huang C Q, Zhang X Y, et al . Preliminary screening of bermudagrass ( Cynodon dactylon ) for salt-tolerance. Chinese Journal of Tropical Agriculture, 2010, 30(4): 20-24.
周霞, 黄春琼, 张绪元, 等. 狗牙根耐盐性材料初步筛选. 热带农业科学, 2010, 30(4): 20-24.
[21] Wang T L, Han Z S, Pang M T, et al . Effect of NaCl stress on physiological characteristics of bermudagrass. Grassland and Turf, 2015, 35(1): 63-67.
王太亮, 韩兆胜, 庞妙甜, 等. NaCl胁迫对狗牙根生理生化特性的影响. 草原与草坪, 2015, 35(1): 63-67.
[22] Verma O P S, Yadava R B R. Salt tolerance of some oats ( Avena sativa L.) varieties at germination and seedling stage. Journal of Agronomy and Crop Science, 1986, 156(2): 123-127.
[23] Khan A A, Rao S A, McNeilly T. Assessment of salinity tolerance based upon seedling root growth response functions in maize ( Zea mays L.). Euphytica, 2003, 131(1): 81-89.
[24] Sun Z J, Li P Y, Abulaiti, et al . Evaluation on the salt tolerance of 38 germplasm resources of Elytrigria repens at seed germination stage. Pratacultural Science, 2012, 29(7): 1105-1113.
孙宗玖, 李培英, 阿不来提, 等. 种子萌发期38份偃麦草种质耐盐性评价. 草业科学, 2012, 29(7): 1105-1113.
[25] Han X F, Li C W, Jia Z K. The study on salt tolerance of different alfalfa varieties in germinating period. Acta Botanica Boreali-Occidentalia Sinica, 2003, 23(4): 597-602.
韩清芳, 李崇巍, 贾志宽. 不同苜蓿品种种子萌发期耐盐性的研究. 西北植物学报, 2003, 23(4): 597-602.
[26] Yuan X H, Wu J Y, Yang X J, et al . Assessment of salt tolerance of ornamental grasses at germination stage and seedling stage based on LC 50 . Chinese Journal of Grassland, 2012, 34(6): 49-53.
袁小环, 武菊英, 杨学军, 等. 基于半致死浓度的观赏草萌发期和幼苗期耐盐性评价. 中国草地学报, 2012, 34(6): 49-53.
[27] Chen X, Zhang Z W, Wu B. Comprehensive evaluation of salt tolerance and screening for salt tolerant accessions of naked oat at germination stage. Scientia Agricultura Sinica, 2014, 47(10): 2038-2046.
陈新, 张宗文, 吴斌. 裸燕麦萌发期耐盐性综合评价与耐盐种质筛选. 中国农业科学, 2014, 47(10): 2038-2046.
[28] Zhang Y H, Abulaiti, Li P Y. Study on morphological diversity of Xinjiang wild bermudagrass. Journal of Xinjing Agricultural University, 2011, 34(1): 6-11.
张延辉, 阿不来提, 李培英. 新疆野生狗牙根形态多样性研究. 新疆农业大学学报, 2011, 34(1): 6-11.
[29] Sun Z J, Li P Y, Abulaiti, et al . Evaluation of drought resistance of 50 Cynodon dactylon germplasms at germinating stage under PEG stress. Arid Zone Research, 2013, 30(3): 497-504.
孙宗玖, 李培英, 阿不来提, 等. 外源脱落酸对抗寒性狗牙根抗氧化酶活性的影响. 干旱区研究, 2013, 30(3): 497-504.
[30] Zong J Q, Gao Y Z, Chen J B, et al . Assessment of salinity tolerance of Miscanthus scchariflora germplasm during germination period. Acta Agrestia Sinica, 2013, 21(6): 1148-1156.
宗俊勤, 高艳芝, 陈静波, 等. 荻不同资源种子萌发期抗盐性评价. 草地学报, 2013, 21(6): 1148-1156.
[31] Yu X J, Xiao H, Xu C L, et al . Comparative study on drought resistance and salt tolerance of Medicago ruthenica and Medicago varia at seed germination period. Journal of Plant Genetic Resources, 2015, 16(2): 405-410.
鱼小军, 肖红, 徐长林, 等. 扁蓿豆和苜蓿种子萌发期抗旱性和耐盐性比较. 植物遗传资源学报, 2015, 16(2): 405-410.
[32] Zhang G W, Lu H L, Zhang L, et al . Salt tolerance evaluation of cotton ( Gossypium hirsutum ) at its germinating and seedling stages and selection of related indices. Chinese Journal of Applied Ecology, 2011, 22(8): 2045-2053.
张国伟, 路海玲, 张雷, 等. 棉花萌发期和苗期耐盐性评价及耐盐指标筛选. 应用生态学报, 2011, 22(8): 2045-2053.
[33] Li P Y, Sun Z J. Evaluation on the resistance of germplasm resources of 33 Elytrigria repens during seed germination period. Pratacultural Science, 2015, 32(4): 593-600.
李培英, 孙宗玖. 33份偃麦草种质芽期耐盐性评价. 草业科学, 2015, 32(4): 593-600.
[34] Liu Z, Wang Z F, Yu H Z, et al . Study on salt tolerance of different alfalfa varieties in germinating period. Journal of Yunnan Agricultural University, 2010, 25(3): 359-363.
刘卓, 王志峰, 于洪柱, 等. 不同苜蓿品种种子萌发期耐盐性的研究. 云南农业大学学报, 2010, 25(3): 359-363.
[35] Dudeck A E, Singh S, Giordano C E, et al . Effects of sodium chloride on Cynodon turf grasses. Agronomy Journal, 1983, 75: 927-930.
[36] Chen X, Guo H L, Chen J B, et al . Comparison of salinity tolerance evaluation system of zoysiagrass. Pratacultural Science, 2014, 31(6): 1052-1057.
陈宣, 郭海林, 陈静波, 等. 结缕草属植物两种耐盐评价方法的比较. 草业科学, 2014, 31(6): 1052-1057.
[37] Wu W C, Qu Y Y, Gao W W, et al . Evaluation of cotton stress resistance based on the responses of the photosynthetic indexes to salt and drought stress. Xinjiang Agricultural Sciences, 2016, 53(9): 1569-1579.
吴文超, 曲延英, 高文伟, 等. 不同棉花品种对盐、旱胁迫的光合响应及抗逆性评价. 新疆农业科学, 2016, 53(9): 1569-1579.
[38] Wang R F, Wang T M, Jin X M, et al . Evaluation of salt tolerance of 11 approved alfalfa cultivars at seed germination stage. Pratacultural Science, 2012, 29(2): 213-218.
王瑞峰, 王铁梅, 金晓明, 等. 11个审定苜蓿品种种子萌发期耐盐性评价. 草业科学, 2012, 29(2): 213-218.