微生态制剂对断奶仔猪生长性能、器官重及其胃肠道发育的影响

doi:10.11686/cyxb2016438

摘要/Abstract

摘要： 通过给苏淮断奶仔猪饲喂微生态制剂(植物乳杆菌为主)研究其对断奶仔猪生长性能、器官重及其胃肠道发育的影响,分析仔猪生长性能与器官重、小肠形态、肠道pH的相关性,探讨微生态制剂缓解仔猪断奶应激的可能机制。选取健康、胎次和体重相近[(9.34±0.33) kg]的144头28日龄苏淮断奶仔猪,随机分为对照组(基础日粮)、抗生素组(基础日粮+抗生素)、微生态制剂组(基础日粮+微生态制剂),每组6个重复,每个重复8头仔猪。预试期为3 d,试验于32日龄开始,59日龄结束时屠宰采样。结果表明,与对照组相比,微生态制剂可显著提高断奶仔猪平均日增重(ADG)和平均日采食量(ADFI)(P<0.05),降低料重比(F/G)和腹泻率(P<0.05);显著提高(P<0.05)断奶仔猪脾脏相对重量;显著增加断奶仔猪十二指肠绒毛高度(P<0.05),显著降低十二指肠、空肠、回肠绒隐比(P<0.05),十二指肠、空肠、回肠隐窝深度有下降趋势,但差异不显著(P>0.05);显著降低(P<0.05)断奶仔猪盲肠、结肠pH。与抗生素组相比,微生态制剂组对各指标无显著影响(P>0.05)。相关性分析显示,ADG增加与肝脏相对重量增加显著相关(P<0.05);ADFI增加与十二指肠和脾脏相对重量、回肠绒隐比增加显著相关(P<0.05),与结肠和盲肠pH、回肠隐窝深度降低显著相关(P<0.05);F/G降低与结肠相对重量增加呈显著相关(P<0.05);腹泻率下降与十二指肠相对重、脾脏相对重量、十二指肠和空肠绒隐比增加呈显著相关(P<0.05)。由此看出,微生态制剂可通过增加器官重,改善肠道形态,降低胃肠道pH,提高断奶仔猪生长性能、降低腹泻率,缓解断奶应激对仔猪的影响,该微生态制剂具有替代抗生素的潜力。

Abstract: The objective of this study was to investigate the effects of probiotics on the growth performance, relative organ weight, and intestinal development of weaned piglets, to determine whether probiotics can ease the weaning transition. A total of 144 Suhuai weaned piglets (9.34±0.33 kg) at an age of 28 days were randomly assigned to three groups; control group (basal diet), antibiotics group (basal diet+antibiotics), and probiotic group (basal diet+probiotic). Each group had six replicates, with eight piglets per replicate. The experiment started at 32 days after birth and ended at 59 days after birth. At 59 days, one piglet from each replicate was slaughtered for sampling. Compared with the control, the probiotic treatment significantly increased the average daily gain (ADG) and average daily feed intake (ADFI), and decreased the feed/gain ratio (F/G) and diarrhea rate after weaning (P<0.05). It also significantly increased ADG of the spleen and improved villus development in the duodenum, and reduced the villus height/crypt depth (V/C) of the duodenum, jejunum, and ileum after weaning (P<0.05). Compared with the control, the probiotic treatment significantly decreased the pH of the cecum and colon. A correlation analysis showed that the increased ADG was associated with increased relative weight of the liver. The increased ADFI was strongly related to the increased relative weight of the duodenum and spleen and the increased V/C of the jejunum. The decreased F/G was correlated with the increased relative weight of the colon. The decreased diarrhea rate was correlated with the increased relative weight of the duodenum and spleen, and with the increased V/C of the duodenum and jejunum. The findings imply that a probiotic can relieve weaning stress in piglets by improving their growth performance and decreasing the diarrhea rate via their effects on small intestine morphology, organ relative weight, and gastrointestinal pH. Probiotics could be used as alternatives to antibiotics during the weaning of piglets.

李雪莉, 王超, 虞徳夫, 丁立人, 朱伟云, 杭苏琴. 微生态制剂对断奶仔猪生长性能、器官重及其胃肠道发育的影响[J]. 草业学报, 2017, 26(8): 192-199.

LI Xue-Li, WANG Chao, YU De-Fu, DING Li-Ren, ZHU Wei-Yun, HANG Su-Qin. Effects of probiotics on the growth performance, organ relative weight, and intestine development of weaned piglets[J]. Acta Prataculturae Sinica, 2017, 26(8): 192-199.

参考文献

[1] Lall S J P, Bosi P, Smidt H, et al . Weaning-A challenge to gut physiologists. Livestock Science, 2007, 108(1/3): 82-93.
[2] Cromwell G L. Why and how antibiotics are used in swine production. Animal Biotechnology, 2002, 13(1): 7-27.
[3] Thacker P A. A review. Journal of Animal Science and Biotechnology, 2013, 4(1): 1-12.
[4] Gong L F. Study on Effects of Probiotics on Production Performance and Immune Function of Weaning Piglets[D]. Changsha: Hunan Agriculture University, 2010.
龚路峰. 微生态制剂对断奶仔猪生产性能和免疫功能的影响[D]. 长沙: 湖南农业大学, 2010.
[5] Li X F, Wang W M, Zheng Q H, et al . Effect of probiotics replacing of antibiotic on production and gastrointestinal microflora. Animal Husbandry and Veterinary Medicine, 2008, 40(10): 55-57.
李小飞, 王为民, 郑秋红, 等. 微生态制剂替代抗生素对猪生产性能和胃肠道微生物数量的影响. 畜牧与兽医, 2008, 40(10): 55-57.
[6] Lee S H, Ingale S L, Kim J S, et al . Effects of dietary supplementation with Bacillus subtilis LS 1-2 fermentation biomass on growth performance, nutrient digestibility, cecal microbiota and intestinal morphology of weanling pig. Animal Feed Science & Technology, 2013, 188(1/2): 78-84.
[7] Frantz N Z, Nelssen J L, Derouchey J M, et al . Effects of a Prebiotic, Inulin, and a Direct Fed Microbial on Growth Performance of Weanling Pigs[C]. Kansas: Kansas State University, 2003.
[8] Nagpal R, Kumar A, Kumar M, et al . Probiotics, their health benefits and applications for developing healthier foods: a review. Fems Microbiology Letters, 2012, 334(1): 1-15.
[9] Li C B. The Perfomance and Microbial Community of Piglets before and After Weaning Response to Lactobacillus plantarum and Lactobacillus casei [D]. Nanjing: Nanjing Agricultural University, 2014.
李晨博. 植物乳杆菌和干酪乳杆菌对仔猪生长相关指标及其肠道微生物菌群多样性的影响[D]. 南京: 南京农业大学, 2014.
[10] Fang Z G. Effect of Different Fat Sources in Diet on Intestinal Development and Intestinal Microbiota of Piglets[D]. Nanjing: Nanjing Agricultural University, 2012.
房正国. 不同脂肪来源的日粮对仔猪肠道发育及肠道微生物区系的影响[D]. 南京: 南京农业大学, 2012.
[11] Liu Y L, Huang J J, Fan W, et al . Effect of L-arginine on growth performance, blood biochemical measurements and visceral weight of weaned pigs after lipopolysaccharide challenge. Chinese Journal of Animal Nutrition, 2008, 20(2): 140-145.
刘玉兰, 黄晶晶, 范伟, 等. L-精氨酸对脂多糖刺激断奶仔猪生产性能、血液生化指标和内脏器官重量的影响. 动物营养学报, 2008, 20(2): 140-145.
[12] Wang G Q, Zhang T W, Liu C Q, et al . Probiotics, their health benefits and applications for developing healthier foods: a review. Feed Reasearch, 2016, (10): 24-27.
王国强, 张天伟, 刘超齐, 等. 微生态制剂对哺乳仔猪生长性能及粪便中微生物区系的影响. 饲料研究, 2016, (10): 24-27.
[13] Hu Y, Dun Y, Li S, et al . Effects of Bacillus subtilis KN-42 on growth performance, diarrhea and faecal bacterial flora of weaned piglets. Asian Australasian Journal of Animal Sciences, 2014, 27(8): 1131-1140.
[14] Zhao P Y, Kim I H. Effect of direct-fed microbial on growth performance, nutrient digestibility, fecal noxious gas emission, fecal microbial flora and diarrhea score in weanling pigs. Animal Feed Science & Technology, 2014, 200(1): 86-92.
[15] Suo C, Yin Y, Wang X, et al . Effects of Lactobacillus plantarum ZJ316 on pig growth and pork quality. Bmc Veterinary Research, 2012, 8(1): 1-12.
[16] Xian Y H, Zhao X Y, Li C B, et al . Effects of Lactobacillus plantarum and Lactobacillus casei on growth performance, organ index and intestinal morphology of piglets. Chinese Journal of Animal Nutrition, 2015, 27(12): 3805-3811.
县怡涵, 赵秀英, 李晨博, 等. 植物乳杆菌和干酪乳杆菌对仔猪生长性能、器官指数及小肠形态的影响. 动物营养学报, 2015, 27(12): 3805-3811.
[17] Takahashi S, Egawa Y, Simojo N, et al . Oral administration of Lactobacillus plantarum strain Lq80 to weaning piglets stimulates the growth of indigenous Lactobacilli to modify the Lactobacillal population. Journal of General & Applied Microbiology, 2007, 53(6): 325-332.
[18] Xiao Z D. Comparison study of feed probiotics and feed antibiotics. Journal of Jilin Agricultural University, 2002, 24(3): 1-6.
肖振铎. 饲用益生素与抗生素的比较研究. 吉林农业大学学报, 2002, 24(3): 1-6.
[19] Li Z F, Zhang S. The progress and prospect of fundamental research of the spleen. Journal of Xi’an Jiaotong University: Medical Sciences Editon, 2008, 29(1): 1-6.
李宗芳, 张澍. 脾脏的基础研究进展与展望. 西安交通大学学报: 医学版, 2008, 29(1): 1-6.
[20] Wang Y, Yang W R, Zhang G G. Effects of Enterococcus faecium on growth performance, intestinal flora and immune function of weaner piglets. Chinese Journal of Animal Nutrition, 2013, 25(5): 1069-1076.
王永, 杨维仁, 张桂国. 饲粮中添加屎肠球菌对断奶仔猪生长性能、肠道菌群和免疫功能的影响. 动物营养学报, 2013, 25(5): 1069-1076.
[21] Macpherson A J, Harris N L. Interactions between commensal intestinal bacteria and the immune system. Nature Reviews Immunology, 2004, 4(6): 478-485.
[22] Roura A E, Homedes J, Klasing K C. Prevention of immunologic stress contributes to the growth-permitting ability of dietary antibiotics in chicks. Journal of Nutrition, 1992, 122(12): 2383-2390.
[23] Yang K M, Jiang Z Y, Zheng C T, et al . Effect of Lactobacillus plantarum on diarrhea and intestinal barrier function of young piglets challenged with enterotoxigenic Escherichia coli K88. Journal of Animal Science, 2014, 92(4): 1496-1503.
[24] Efird R C, Armstrong W D, Herman D L. The development of digestive capacity in young pigs: effects of age and weaning system. Journal of Animal Science, 1982, 55(6): 1380-1387.
[25] Lu J J, Xu Z R. Approach on the mechanisms of effects of chlortetracycline in the diet on intestinal morphology in weaning piglets. Acta Agriculturae Zhejiangensis, 2007, 19(1): 15-19.
卢建军, 许梓荣. 日粮抗生素影响断奶仔猪肠道结构的机理研究. 浙江农业学报, 2007, 19(1): 15-19.
[26] Xin N, Zhang N F, Diao Q Y, et al . Effects of bacillus preparation on growth performance and gastrointesinal development of weaned piglets. Acta Veterinaria et Zootechnica Sinica, 2012, 43(6): 901-908.
辛娜, 张乃锋, 刁其玉, 等. 芽孢杆菌制剂对断奶仔猪生长性能、胃肠道发育的影响. 畜牧兽医学报, 2012, 43(6): 901-908.
[27] Wang K, Ma J L, Lai T, et al . Effects of Lactobacillus on intestinal physical indices of weaned piglets. Chinese Journal of Veterinary Medicine, 2008, 44(9): 21-23.
王凯, 马金磊, 赖婷, 等. 乳酸菌剂对断奶仔猪肠道生理指标的影响. 中国兽医杂志, 2008, 44(9): 21-23.
[28] Ogawa M, Shimizu K, Nomoto K, et al . Inhibition of in vitro growth of Shiga toxin-producing Escherichia coli O157: H7 by probiotic Lactobacillus strains due to production of lactic acid. International Journal of Food Microbiology, 2001, 68(1): 135-140.